{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:15:49Z","timestamp":1787030149343,"version":"build-2736575974"},"reference-count":61,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"project \u201cIncreasing the impact of excellence research on the capacity for innovation and technology transfer within USV Timisoara\u201d","award":["6PFE"],"award-info":[{"award-number":["6PFE"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. The juvenile forms, which are found in the small intestine, are more pathogenic compared to the adults. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. In the last decade, paramphistomosis has been described as an emerging parasitic disease in Europe. This study aimed to identify the rumen fluke species in wild ruminants from western Romania. Fifty-two pre-stomachs obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timi\u0219 and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timi\u0219 County, and one (5.26%) out of 19 samples was positive in Arad County. Subsequent PCR testing revealed that three samples were positive for Calicophoron daubneyi and one for Paramphistomum\u00a0leydeni. The presence of C. daubneyi and P. leydeni in roe deer has not been previously reported in Romania. Two Paramphistomum species\u2014C. daubneyi and P. leydeni\u2014were revealed as the main species of rumen flukes in roe deer from forests in Romania.<\/jats:p>","DOI":"10.3390\/vetsci10100603","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:28:29Z","timestamp":1696318109000},"page":"603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-1013","authenticated-orcid":false,"given":"Sorin","family":"Morariu","sequence":"first","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103t\u0103lin Bogdan","family":"S\u00eerbu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienn Gr\u00e9ta","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department Centre for Bioinformatics, University of Veterinary Medicine Budapest, Budapest Istv\u00e1n u. 2, 1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gheorghe","family":"D\u0103r\u0103bu\u0219","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ion","family":"Oprescu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisa","family":"Mederle","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5484-4685","authenticated-orcid":false,"given":"Marius Stelian","family":"Ilie","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3004-463X","authenticated-orcid":false,"given":"Mirela","family":"Imre","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1536-9874","authenticated-orcid":false,"given":"Beatrice Ana-Maria","family":"S\u00eerbu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1783-2041","authenticated-orcid":false,"given":"Norbert","family":"Solymosi","sequence":"additional","affiliation":[{"name":"Department Centre for Bioinformatics, University of Veterinary Medicine Budapest, Budapest Istv\u00e1n u. 2, 1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiana","family":"Florea","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-882X","authenticated-orcid":false,"given":"Kalman","family":"Imre","sequence":"additional","affiliation":[{"name":"Food Safety Department, University of Life Sciences King Mihai I, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kahl, A., von Samson-Himmelstjerna, G., Krucken, J., and Ganter, M. 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