{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:56:15Z","timestamp":1785884175680,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T00:00:00Z","timestamp":1699401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific and Technological Research Council of Turkey (TUBITAK)","award":["1140636"],"award-info":[{"award-number":["1140636"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study aimed to investigate the relationship between the sperm quality and the osteopontin (OPN) concentration in the prostates of Malakli shepherd dogs. Ejaculates were collected once by digital manipulation from 39 male dogs aged between 2 and 4 years and older. The first and third fractions of the ejaculate were centrifuged at 5000\u00d7 g for 30 min, and supernatants were stored at \u221280 \u00b0C for further analysis of OPN using a double-antibody sandwich method (SEA899CA, Cloude-Clone Corp, Houston, TX, USA). Meanwhile, the second fractions were evaluated for sperm motility, concentration, viability, and rate of abnormal spermatozoa (head, acrosome, midpiece and tail abnormalities). The average concentration of OPN was 8.7 \u00b1 5.2 ng\/mL, and it differed significantly between the 1st 10.4 \u00b1 5.3 ng\/mL and 3rd 7.4 \u00b1 5 ng\/mL fractions. According to ROC (receiver operating characteristic curve) analysis, the OPN concentration had a better diagnostic ability for sperm motility (p &lt; 0.001) than for the rate of abnormal spermatozoa (p &lt; 0.05). Additionally, the OPN concentration was negatively correlated with poor sperm morphology and motility. In conclusion, the OPN concentration in prostate-derived secretions may be a possible marker of sperm quality in dogs. Further research could explore the involvement of OPN in sperm motility during cryopreservation and in vivo fertility.<\/jats:p>","DOI":"10.3390\/vetsci10110646","type":"journal-article","created":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T03:42:40Z","timestamp":1699501360000},"page":"646","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Osteopontin Concentration in Prostates Fractions: A Novel Marker of Sperm Quality in Dogs"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3862-2337","authenticated-orcid":false,"given":"Koray","family":"Tekin","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8436-3332","authenticated-orcid":false,"given":"Efe","family":"Kurtdede","sequence":"additional","affiliation":[{"name":"The Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berrin","family":"Salmano\u011flu","sequence":"additional","affiliation":[{"name":"The Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ongun","family":"Uysal","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2495-2738","authenticated-orcid":false,"given":"Calogero","family":"Stelletta","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Production and Health, University of Padova, Legnaro, 35020 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.theriogenology.2018.04.021","article-title":"Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm","volume":"115","author":"Wallgren","year":"2018","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ohaneje, U.L., Osuagwuh, U.I., Alvarez-Rodr\u00edguez, M., Y\u00e1nez-Ortiz, I., Tabarez, A., and Palomo, M.J. (2021). The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters. Animals, 11.","DOI":"10.3390\/ani11123452"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1439-0531.2009.01447.x","article-title":"Osteopontin in seminal plasma and sperm membrane of dogs","volume":"44","author":"Souza","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_4","first-page":"110","article-title":"Protein patterns of canine seminal plasma","volume":"38","author":"Menezes","year":"2014","journal-title":"Rev. Bras. Reprod. Anim."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.theriogenology.2017.03.016","article-title":"Proteomic characterization of canine seminal plasma","volume":"95","author":"Pinheiro","year":"2017","journal-title":"Theriogenology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/mrd.23312","article-title":"Adhesion molecules in gamete transport, fertilization, early embryonic development, and implantation-role in establishing a pregnancy in cattle: A review","volume":"87","author":"Campanile","year":"2020","journal-title":"Mol. Reprod. Dev."},{"key":"ref_7","first-page":"e919159-1","article-title":"Osteopontin in bone metabolism and bone diseases","volume":"26","author":"Si","year":"2020","journal-title":"Med. Sci. Monit. Int. Med. J. Exp. Clin. Res."},{"key":"ref_8","first-page":"758","article-title":"Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs","volume":"55","author":"Abedin","year":"2021","journal-title":"Indian J. Anim. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1530\/REP-06-0228","article-title":"Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization","volume":"133","author":"Erikson","year":"2007","journal-title":"Reproduction"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1002\/j.1939-4640.2000.tb03396.x","article-title":"Osteopontin gene expression in the Holstein bull reproductive tract","volume":"21","author":"Day","year":"2000","journal-title":"J. Androl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tice.2016.07.003","article-title":"Age-associated and tissue-specific expression of osteopontin in male Hu sheep reproductive tract","volume":"48","author":"Zhang","year":"2016","journal-title":"Tissue Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"946","DOI":"10.3892\/etm.2021.10378","article-title":"Salinomycin triggers prostate cancer cell apoptosis by inducing oxidative and endoplasmic reticulum stress via suppressing Nrf2 signaling","volume":"22","author":"Yu","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_13","first-page":"1","article-title":"Determination of the Effect of Nutritional Status on PRAL Level in Patients with Nephrolithiasis","volume":"7","author":"Icer","year":"2018","journal-title":"G\u00fcm\u00fc\u015fhane Univ. J. Health Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12610-018-0071-5","article-title":"Understanding the seminal plasma proteome and its role in male fertility","volume":"28","author":"Camargo","year":"2018","journal-title":"Basic Clin. Androl."},{"key":"ref_15","first-page":"3","article-title":"Seminal plasma proteins and fertility indexes in the bull. The case for osteopontin","volume":"2","author":"Moura","year":"2005","journal-title":"Anim. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1095\/biolreprod66.5.1437","article-title":"Osteopontin expression and regulation in the testis, efferent ducts, and epididymis of rats during postnatal development through to adulthood","volume":"66","author":"Luedtke","year":"2002","journal-title":"Biol. Reprod."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Stenhouse, C., Cortes-Araya, Y., Donadeu, F.X., and Ashworth, C.J. (2022). Associations between testicular development and fetal size in the pig. J. Anim. Sci. Biotechnol., 13.","DOI":"10.1186\/s40104-022-00678-3"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/S0093-691X(99)00178-8","article-title":"Two-dimensional polyacrylamide gel electrophoresis of equine seminal plasma proteins and their correlation with fertility","volume":"52","author":"Brandon","year":"1999","journal-title":"Theriogenology"},{"key":"ref_19","unstructured":"Amedeo, S., Cerruti, F., Capucchio, M.T., Palmerini, D., Apicella, M., Giberti, M., and Mioletti, S. (2009, January 12\u201313). Osteopontin expression in normal and criptorchid boar testes: Preliminary results. Proceedings of the Atti della Societ\u00e0 Italiana di Patologia ed Allevamento dei Suini, XXXV Meeting Annuale, Modena, Italy."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.molmed.2011.02.004","article-title":"Dog models of naturally occurring cancer","volume":"17","author":"Rowell","year":"2011","journal-title":"Trends Mol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/rda.14100","article-title":"Comparison of two staining techniques on the manual and automated canine sperm morphology analysis","volume":"57","author":"Surmacz","year":"2022","journal-title":"Reprod. Domest. Anim."},{"key":"ref_22","first-page":"266","article-title":"Infertility in male dogs: Recent advances","volume":"35","author":"Fontbonne","year":"2011","journal-title":"Rev. Bras. Reprod. Anim."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1242\/dev.003004","article-title":"Akt mediates self-renewal division of mouse spermatogonial stem cells","volume":"134","author":"Lee","year":"2007","journal-title":"Development"},{"key":"ref_24","first-page":"e13905","article-title":"Bovine seminal plasma osteopontin: Structural modelling, recombinant expression and its relationship with semen quality","volume":"53","author":"Mosena","year":"2021","journal-title":"Andrologia"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Michos, I., Tsantarliotou, M., Boscos, C.M., Tsousis, G., Basioura, A., and Tzika, E.D. (2021). Effect of boar sperm proteins and quality changes on field fertility. Animals, 11.","DOI":"10.3390\/ani11061813"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"891","DOI":"10.22271\/j.ento.2020.v8.i6l.7956","article-title":"Ameliorative effects of osteopontin on sperm morphology of frozen thawed buffalo semen treated with sodium nitroprusside","volume":"8","author":"Viswam","year":"2020","journal-title":"J. Entomol. Zool. Stud."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"188","DOI":"10.2164\/jandrol.109.007583","article-title":"Seminal plasma proteins as potential markers of relative fertility in boars","volume":"31","author":"Novak","year":"2010","journal-title":"J. Androl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.theriogenology.2014.10.033","article-title":"Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)","volume":"83","author":"Waheed","year":"2015","journal-title":"Theriogenology"},{"key":"ref_29","first-page":"648","article-title":"Detection of osteopontin transcript in seminal plasma and its association with post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis)","volume":"51","author":"Baruah","year":"2017","journal-title":"Indian J. Anim. Res."},{"key":"ref_30","first-page":"2","article-title":"Signalling mechanisms in mammalian sperm motility","volume":"96","author":"Freitas","year":"2017","journal-title":"Biol. Reprod."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/11\/646\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:19:34Z","timestamp":1760131174000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/11\/646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,8]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["vetsci10110646"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10110646","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,8]]}}}