{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:40:36Z","timestamp":1760150436682,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T00:00:00Z","timestamp":1700524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Melanomas are tumors arising from externally uncontrolled melanocytes that produce varying amounts of melanin. In this study, we report a case of melanoma with neurological impairment without evidence of cutaneous neoplastic lesions in an adult buffalo in the state of Par\u00e1, Brazil. Clinically, the buffalo exhibited apathy, decreased mandibular tone, and occasionally an open mouth with motor incoordination, and eventually succumbed to the condition. Necropsy revealed multifocal tumor masses in the brain, pituitary gland, trigeminal ganglion, and spinal cord. The neoplastic cells showed strong positive signals for vimentin, Melan-A, PNL-2, and SOX10. The diagnosis was made via necropsy, histopathology, and positive immunostaining for Melan-A and PNL-2, which are specific markers for melanocyte identification.<\/jats:p>","DOI":"10.3390\/vetsci10120662","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T01:48:45Z","timestamp":1700531325000},"page":"662","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extracutaneous Melanotic Melanoma with Nervous System Involvement in a Buffalo (Bubalus bubalis)"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8915-5735","authenticated-orcid":false,"given":"Jos\u00e9 Diomedes","family":"Barbosa","sequence":"first","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Correia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiologia e Sa\u00fade P\u00fablica (DESP), Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop\u00e9dica 23890-000, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Magno Chaves","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique dos Anjos","family":"Bomjardim","sequence":"additional","affiliation":[{"name":"Instituto de Estudos do Tr\u00f3pico \u00damido (IETU), Universidade Federal do Sul e Sudeste do Par\u00e1 (UNIFESSPA), Xinguara 68557-335, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiane Teles Albernaz","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Dutra","family":"Duarte","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Alcides Sarmento","family":"da Silveira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-8948","authenticated-orcid":false,"given":"Nat\u00e1lia da Silva e Silva","family":"Silveira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7837-4246","authenticated-orcid":false,"given":"Camila Cordeiro","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alu\u00edzio Ot\u00e1vio Almeida","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1 (UFPA), Castanhal 68740-970, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1870-9945","authenticated-orcid":false,"given":"Anibal","family":"Armi\u00e9n","sequence":"additional","affiliation":[{"name":"California Animal Health & Food Safety Laboratory System, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilene de Farias","family":"Brito","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiologia e Sa\u00fade P\u00fablica (DESP), Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop\u00e9dica 23890-000, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"ref_1","unstructured":"I.B.B.E.\u2014Instituto Brasileiro de Geografia e Estat\u00edstica, and de Bubalinos, R. 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