{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:49:58Z","timestamp":1790833798738,"version":"4.1.0"},"reference-count":89,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011091","name":"Belgian Federal Public Service for Health, Food Chain Safety and Environment","doi-asserted-by":"publisher","award":["RT 18\/6 DECHAHEV"],"award-info":[{"award-number":["RT 18\/6 DECHAHEV"]}],"id":[{"id":"10.13039\/501100011091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Hepatitis E virus is a prominent cause of viral hepatitis worldwide. In Western countries, most infections are asymptomatic. However, acute self-limiting hepatitis and chronic cases in immunocompromised individuals can occur. Studying HEV is challenging due to its difficulty to grow in cell culture. Consequently, the detection of the virus mainly relies on RT-qPCR, which cannot differentiate between infectious and non-infectious particles. To overcome this problem, methods assessing viral integrity offer a possible solution to differentiate between intact and damaged viruses. This study aims at optimizing existing HEV cell culture models and RT-qPCR-based assays for selectively detecting intact virions to establish a reliable model for assessing HEV infectivity. In conclusion, these newly developed methods hold promise for enhancing food safety by identifying approaches for inactivating HEV in food processing, thereby increasing food safety measures.<\/jats:p>","DOI":"10.3390\/vetsci10120676","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T07:56:10Z","timestamp":1701071770000},"page":"676","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1357-8090","authenticated-orcid":false,"given":"Tatjana","family":"Locus","sequence":"first","affiliation":[{"name":"Fisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, Belgium"},{"name":"Sciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, Belgium"},{"name":"Laboratory of Experimental Medicine and Pediatrics, Viral Hepatitis Research Group, University of Antwerp, Drie Eikenstraat 655, 2650 Edegem, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8068-4809","authenticated-orcid":false,"given":"Ellen","family":"Lambrecht","sequence":"additional","affiliation":[{"name":"Fisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Lamoral","sequence":"additional","affiliation":[{"name":"Sciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sjarlotte","family":"Willems","sequence":"additional","affiliation":[{"name":"Fisheries and Food, Technology and Food Unit, Flemish Research Institute for Agriculture (ILVO), Brusselsesteenweg 370, 9090 Melle, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Van Gucht","sequence":"additional","affiliation":[{"name":"Sciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0572-8741","authenticated-orcid":false,"given":"Thomas","family":"Vanwolleghem","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Medicine and Pediatrics, Viral Hepatitis Research Group, University of Antwerp, Drie Eikenstraat 655, 2650 Edegem, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0775-7888","authenticated-orcid":false,"given":"Michael","family":"Peeters","sequence":"additional","affiliation":[{"name":"Sciensano, Infectious Diseases in Humans, Viral Diseases, Engelandstraat 642, 1180 Ukkel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"ref_1","unstructured":"ECDC (2019). Options for National Testing and Surveillance for Hepatitis E Virus in the EU\/EEA\u2014Operational Guidance, ECDC."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nrgastro.2017.150","article-title":"Hepatitis e Virus: Advances and Challenges","volume":"15","author":"Nimgaonkar","year":"2018","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jhep.2022.08.033","article-title":"Viral Clade Is Associated with Severity of Symptomatic Genotype 3 Hepatitis E Virus Infections in Belgium, 2010\u20132018","volume":"78","author":"Peeters","year":"2023","journal-title":"J. Hepatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e00822-17","DOI":"10.1128\/JVI.00822-17","article-title":"Characterization of the Quasi-Enveloped Hepatitis E Virus Particles Released by the Cellular Exosomal Pathway","volume":"91","author":"Nagashima","year":"2017","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1007\/s00705-008-0179-6","article-title":"Monoclonal Antibodies Raised against the ORF3 Protein of Hepatitis e Virus (HEV) Can Capture HEV Particles in Culture Supernatant and Serum but Not Those in Feces","volume":"153","author":"Takahashi","year":"2008","journal-title":"Arch. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1099\/jgv.0.000940","article-title":"ICTV Virus Taxonomy Profile: Hepeviridae","volume":"98","author":"Purdy","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1099\/jgv.0.001435","article-title":"Update: Proposed Reference Sequences for Subtypes of Hepatitis E Virus (Species Orthohepevirus A)","volume":"101","author":"Smith","year":"2020","journal-title":"J. Gen. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mib.2020.07.004","article-title":"Hepatitis E Virus: Host Tropism and Zoonotic Infection","volume":"59","author":"Wang","year":"2021","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1053\/j.gastro.2015.10.048","article-title":"Chronic Infection with Camelid Hepatitis e Virus in a Liver Transplant Recipient Who Regularly Consumes Camel Meat and Milk","volume":"150","author":"Lee","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s12560-018-9342-8","article-title":"Hiding in Plain Sight? It\u2019s Time to Investigate Other Possible Transmission Routes for Hepatitis E Virus (HEV) in Developed Countries","volume":"10","author":"King","year":"2018","journal-title":"Food Environ. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.coviro.2018.11.006","article-title":"Hepatitis E Virus: Reasons for Emergence in Humans","volume":"34","author":"Sooryanarain","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1111\/tbed.12435","article-title":"Belgian Wildlife as Potential Zoonotic Reservoir of Hepatitis E Virus","volume":"64","author":"Thiry","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12560-021-09461-5","article-title":"The Foodborne Transmission of Hepatitis E Virus to Humans","volume":"13","author":"Treagus","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"van der Honing, R.W.H., van Coillie, E., Antonis, A.F.G., and van der Poel, W.H.M. (2011). First Isolation of Hepatitis E Virus Genotype 4 in Europe through Swine Surveillance in the Netherlands and Belgium. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022673"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ijfoodmicro.2019.02.018","article-title":"Monitoring of Pork Liver and Meat Products on the Dutch Market for the Presence of HEV RNA","volume":"296","author":"Boxman","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Boxman, I.L.A., Jansen, C.C.C., Zwartkruis-Nahuis, A.J.T., H\u00e4gele, G., Sosef, N.P., and Dirks, R.A.M. (2020). Detection and Quantification of Hepatitis E Virus RNA in Ready to Eat Raw Pork Sausages in the Netherlands. Int. J. Food Microbiol., 333.","DOI":"10.1016\/j.ijfoodmicro.2020.108791"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/jvh.13396","article-title":"Hepatitis E Virus Genome Detection in Commercial Pork Livers and Pork Meat Products in Germany","volume":"28","author":"Pallerla","year":"2020","journal-title":"J. Viral Hepat."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Locus, T., Lambrecht, E., Peeters, M., Suin, V., Verhaegen, B., Van Hoorde, K., Lamoral, S., Vanwolleghem, T., and Van Gucht, S. (2023). Hepatitis E Virus in Pork Meat Products and Exposure Assessment in Belgium. Int. J. Food Microbiol., 397.","DOI":"10.1016\/j.ijfoodmicro.2023.110198"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/s12560-019-09393-1","article-title":"Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia","volume":"11","author":"Velebit","year":"2019","journal-title":"Food Environ. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s00003-021-01357-7","article-title":"Hepatitis E Virus Cross-Contamination on the Surface of Porcine Livers after Storage in Euro Meat Containers in a German Pig Abattoir","volume":"17","author":"Dzierzon","year":"2022","journal-title":"J. Verbraucherschutz Leb."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.3201\/eid1808.111647","article-title":"Hepatitis e Virus in Pork Food Chain, United Kingdom, 2009\u20132010","volume":"18","author":"Berto","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.jviromet.2012.10.017","article-title":"Replication of Hepatitis E Virus in Three-Dimensional Cell Culture","volume":"187","author":"Berto","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ijfoodmicro.2007.11.068","article-title":"Inactivation of Infectious Hepatitis E Virus Present in Commercial Pig Livers Sold in Local Grocery Stores in the United States","volume":"123","author":"Feagins","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s00705-011-1153-2","article-title":"A549 and PLC\/PRF\/5 Cells Can Support the Efficient Propagation of Swine and Wild Boar Hepatitis E Virus (HEV) Strains: Demonstration of HEV Infectivity of Porcine Liver Sold as Food","volume":"157","author":"Takahashi","year":"2012","journal-title":"Arch. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.3201\/eid1112.051041","article-title":"Hepatitis E Virus Transmission from Wild Boar Meat","volume":"11","author":"Li","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1111\/j.1537-2995.2008.01722.x","article-title":"A Case of Transfusion-Transmitted Hepatitis e Caused by Blood from a Donor Infected with Hepatitis e Virus via Zoonotic Food-Borne Route","volume":"48","author":"Matsubayashi","year":"2008","journal-title":"Transfusion"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1016\/S0399-8320(07)78342-2","article-title":"H\u00e9patite Virale E Autochtone En France et Consommation de Viande de Porc S\u00e9ch\u00e9e","volume":"31","author":"Deest","year":"2007","journal-title":"Gastroenterol. Clin. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.3201\/eid2011.140791","article-title":"Foodborne Transmission of Hepatitis E Virus from Raw Pork Liver Sausage, France","volume":"20","author":"Renou","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4225","DOI":"10.1128\/AEM.00951-16","article-title":"Thermal Stability of Hepatitis E Virus as Estimated by a Cell Culture Method","volume":"82","author":"Johne","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1007\/s12560-020-09440-2","article-title":"Effect of Sodium Chloride, Sodium Nitrite and Sodium Nitrate on the Infectivity of Hepatitis E Virus","volume":"12","author":"Wolff","year":"2020","journal-title":"Food Environ. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wolff, A., G\u00fcnther, T., Albert, T., Schilling-Loeffler, K., Gadicherla, A.K., and Johne, R. (2020). Stability of Hepatitis E Virus at Different PH Values. Int. J. Food Microbiol., 325.","DOI":"10.1016\/j.ijfoodmicro.2020.108625"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5153","DOI":"10.1128\/AEM.00436-12","article-title":"Thermal Inactivation of Infectious Hepatitis E Virus in Experimentally Contaminated Food","volume":"78","author":"Barnaud","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"947","DOI":"10.4315\/0362-028X.JFP-17-290","article-title":"Evaluation of Heating Conditions for Inactivation of Hepatitis e Virus Genotypes 3 and 4","volume":"81","author":"Imagawa","year":"2018","journal-title":"J. Food Prot."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.antiviral.2019.01.007","article-title":"Cell Culture Systems for the Study of Hepatitis E Virus","volume":"163","author":"Meister","year":"2019","journal-title":"Antivir. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Fu, R.M., Decker, C.C., and Dao Thi, V.L. (2019). Cell Culture Models for Hepatitis E Virus. Viruses, 11.","DOI":"10.3390\/v11070608"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12560-017-9303-7","article-title":"Potential Approaches to Assess the Infectivity of Hepatitis E Virus in Pork Products: A Review","volume":"9","author":"Cook","year":"2017","journal-title":"Food Environ. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martin-Latil, S., Hennechart-Collette, C., Delannoy, S., Guillier, L., Fach, P., and Perelle, S. (2016). Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01183"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2014.01.016","article-title":"Method for HEV Detection in Raw Pig Liver Products and Its Implementation for Naturally Contaminated Food","volume":"176","author":"Guillier","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ijfoodmicro.2014.10.005","article-title":"Detection of Hepatitis E Virus in Pork Liver Sausages","volume":"193","author":"Angeloni","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_40","unstructured":"Cook, N., D\u2019Agostino, M., Clarke, E., and Johne, R. (2016). FSA Project FS301014: A Critical Review of Approaches to Assess the Infectivity of Hepatitis E Virus. A Report to the United Kingdom Food Standards Agency, Fera Science Ltd. (FERA)."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ijfoodmicro.2018.01.015","article-title":"Discrimination of Infectious and Heat-Treated Norovirus by Combining Platinum Compounds and Real-Time RT-PCR","volume":"269","author":"Fraisse","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/1541-4337.12072","article-title":"Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection","volume":"13","author":"Ceuppens","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111831","DOI":"10.1016\/j.envres.2021.111831","article-title":"Discrimination of Non-Infectious SARS-CoV-2 Particles from Fomites by Viability RT-QPCR","volume":"203","author":"Aznar","year":"2022","journal-title":"Environ. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Raymond, P., Paul, S., and Guy, R.A. (2023). Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates. Foods, 12.","DOI":"10.3390\/foods12040826"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"18120","DOI":"10.1038\/s41598-021-97700-x","article-title":"Platinum Chloride-Based Viability RT-QPCR for SARS-CoV-2 Detection in Complex Samples","volume":"11","author":"Randazzo","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Randazzo, W., Vasquez-Garc\u00eda, A., Aznar, R., and S\u00e1nchez, G. (2018). Viability RT-QPCR to Distinguish between HEV and HAV with Intact and Altered Capsids. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01973"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2016.04.010","article-title":"Evaluation of Viability PCR Performance for Assessing Norovirus Infectivity in Fresh-Cut Vegetables and Irrigation Water","volume":"229","author":"Randazzo","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2017.11.011","article-title":"Optimization of PMAxx Pretreatment to Distinguish between Human Norovirus with Intact and Altered Capsids in Shellfish and Sewage Samples","volume":"266","author":"Randazzo","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"106818","DOI":"10.1016\/j.foodcont.2019.106818","article-title":"Viability RT-QPCR to Detect Potentially Infectious Enteric Viruses on Heat-Processed Berries","volume":"107","author":"Chen","year":"2020","journal-title":"Food Control"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Leifels, M., Dan, C., Sozzi, E., Shoults, D.C., Wuertz, S., Mongkolsuk, S., and Sirikanchana, K. (2020). Capsid Integrity Quantitative PCR to Determine Virus Infectivity in Environmental and Food Applications; a Systematic Review. medRxiv.","DOI":"10.1101\/2020.05.08.20095364"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.ijheh.2015.02.003","article-title":"Use of Ethidium Monoazide and Propidium Monoazide to Determine Viral Infectivity upon Inactivation by Heat, UV- Exposure and Chlorine","volume":"218","author":"Leifels","year":"2015","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Leifels, M., Shoults, D., Wiedemeyer, A., Ashbolt, N.J., Sozzi, E., Hagemeier, A., and Jurzik, L. (2019). Capsid Integrity QPCR-an Azo-Dye Based and Culture-Independent Approach to Estimate Adenovirus Infectivity after Disinfection and in the Aquatic Environment. Water, 11.","DOI":"10.3390\/w11061196"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/AEM.01039-07","article-title":"Detection of Murine Norovirus 1 by Using Plaque Assay, Transfection Assay, and Real-Time Reverse Transcription-PCR before and after Heat Exposure","volume":"74","author":"Baert","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1186\/1743-422X-8-487","article-title":"Thermal Stability of Hepatitis e Virus Assessed by a Molecular Biological Approach","volume":"8","author":"Schielke","year":"2011","journal-title":"Virol. J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/AEM.01797-09","article-title":"Effects of Technological Processes on the Tenacity and Inactivation of Norovirus Genogroup II in Experimentally Contaminated Foods","volume":"76","author":"Mormann","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1111\/jam.13568","article-title":"Enzymatic and Viability RT-QPCR Assays for Evaluation of Enterovirus, Hepatitis A Virus and Norovirus Inactivation: Implications for Public Health Risk Assessment","volume":"124","author":"Monteiro","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s12560-019-09390-4","article-title":"Assessment of the Applicability of Capsid-Integrity Assays for Detecting Infectious Norovirus Inactivated by Heat or UV Irradiation","volume":"11","author":"Walker","year":"2019","journal-title":"Food Environ. Virol."},{"key":"ref_58","first-page":"1008","article-title":"Performance of Pre-RT-QPCR Treatments to Discriminate Infectious Human Rotaviruses and Noroviruses from Heat-Inactivated Viruses: Applications of PMA\/PMAxx, Benzonase and RNase","volume":"124","author":"Oristo","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1099\/vir.0.049858-0","article-title":"New Models of Hepatitis E Virus Replication in Human and Porcine Hepatocyte Cell Lines","volume":"94","author":"Talbot","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1007\/s12560-017-9300-x","article-title":"Detection of Potential Infectious Enteric Viruses in Fresh Produce by (RT)-QPCR Preceded by Nuclease Treatment","volume":"9","author":"Marti","year":"2017","journal-title":"Food Environ. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s12560-009-9016-7","article-title":"Long-Range Reverse Transcription as a Useful Tool to Assess the Genomic Integrity of Norovirus","volume":"1","author":"Wolf","year":"2009","journal-title":"Food Environ. Virol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"6473","DOI":"10.1128\/AEM.02092-14","article-title":"Application of Long-Range and Binding Reverse Transcription-Quantitative PCR to Indicate the Viral Integrities of Noroviruses","volume":"80","author":"Li","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.1021\/acs.est.9b05747","article-title":"UV Disinfection of Human Norovirus: Evaluating Infectivity Using a Genome-Wide PCR-Based Approach","volume":"54","author":"Rockey","year":"2020","journal-title":"Environ. Sci. Technol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s12560-020-09454-w","article-title":"Optimisation of a PMAxxTM-RT-QPCR Assay and the Preceding Extraction Method to Selectively Detect Infectious Murine Norovirus Particles in Mussels","volume":"13","author":"Razafimahefa","year":"2021","journal-title":"Food Environ. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1111\/jam.12169","article-title":"Long-Range Quantitative PCR for Determining Inactivation of Adenovirus 2 by Ultraviolet Light","volume":"114","author":"Bounty","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/jvh.12157","article-title":"An ORF1-Rearranged Hepatitis e Virus Derived from a Chronically Infected Patient Efficiently Replicates in Cell Culture","volume":"21","author":"Johne","year":"2014","journal-title":"J. Viral Hepat."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Schemmerer, M., Apelt, S., Trojnar, E., Ulrich, R.G., Wenzel, J.J., and Johne, R. (2016). Enhanced Replication of Hepatitis E Virus Strain 47832C in an A549-Derived Subclonal Cell Line. Viruses, 8.","DOI":"10.3390\/v8100267"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Capelli, N., Dubois, M., Pucelle, M., Da Silva, I., Lhomme, S., Abravanel, F., Chapuy-Regaud, S., and Izopet, J. (2020). Optimized Hepatitis e Virus (HEV) Culture and Its Application to Measurements of HEV Infectivity. Viruses, 12.","DOI":"10.3390\/v12020139"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Chew, N., Situ, J., Wu, S., Yao, W., and Sridhar, S. (2022). Independent Evaluation of Cell Culture Systems for Hepatitis E Virus. Viruses, 14.","DOI":"10.3390\/v14061254"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.1128\/JVI.02804-15","article-title":"Distinct Entry Mechanisms for Nonenveloped and Quasi-Enveloped Hepatitis E Viruses","volume":"90","author":"Yin","year":"2016","journal-title":"J. Virol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"869","DOI":"10.3201\/eid1805.111712","article-title":"Hepatitis E Virus Infection among Solid Organ Transplant Recipients, the Netherlands","volume":"18","author":"Pas","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1002\/jmv.21564","article-title":"Imported and Autochthonous Hepatitis E Virus Strains in Spain","volume":"81","author":"Fogeda","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1073\/pnas.1912307117","article-title":"Robust Hepatitis e Virus Infection and Transcriptional Response in Human Hepatocytes","volume":"117","author":"Todt","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4394","DOI":"10.1128\/JVI.00114-16","article-title":"Hepatitis E Virus (HEV) Genotype 3 Infection of Human Liver Chimeric Mice as a Model for Chronic HEV Infection","volume":"90","author":"Pass","year":"2016","journal-title":"J. Virol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"15655","DOI":"10.1073\/pnas.232137699","article-title":"Infection of a Human Hepatoma Cell Line by Hepatitis B Virus","volume":"99","author":"Gripon","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"10253","DOI":"10.1128\/JVI.01059-06","article-title":"Production of Infectious Hepatitis C Virus by Well-Differentiated, Growth-Arrested Human Hepatoma-Derived Cells","volume":"80","author":"Sainz","year":"2006","journal-title":"J. Virol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e01207-18","DOI":"10.1128\/JVI.01207-18","article-title":"Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes","volume":"93","author":"Capelli","year":"2019","journal-title":"J. Virol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e00478-17","DOI":"10.1128\/JVI.00478-17","article-title":"Entry and Release of Hepatitis C Virus in Polarized Human Hepatocytes","volume":"91","author":"Belouzard","year":"2017","journal-title":"J. Virol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"e00585-21","DOI":"10.1128\/JVI.00585-21","article-title":"The Viral ORF3 Protein Is Required for Hepatitis E Virus Apical Release and Efficient Growth in Polarized Hepatocytes and Humanized Mice","volume":"95","author":"Sari","year":"2021","journal-title":"J. Virol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1038\/s41598-023-29060-7","article-title":"Fetal Bovine Serum, an Important Factor Affecting the Reproducibility of Cell Experiments","volume":"13","author":"Liu","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Ianiro, G., Monini, M., Ammendolia, M.G., De Sabato, L., Ostanello, F., Vaccari, G., and Di Bartolo, I. (2023). In Vitro Replication of Swine Hepatitis E Virus (HEV): Production of Cell-Adapted Strains. Animals, 13.","DOI":"10.3390\/ani13020276"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.virol.2005.08.010","article-title":"Serum-Dependent Expression of Promyelocytic Leukemia Protein Suppresses Propagation of Influenza Virus","volume":"343","author":"Iki","year":"2005","journal-title":"Virology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.chom.2011.03.012","article-title":"The Soluble Serum Protein Gas6 Bridges Virion Envelope Phosphatidylserine to the TAM Receptor Tyrosine Kinase Axl to Mediate Viral Entry","volume":"9","author":"Morizono","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jviromet.2013.06.024","article-title":"Fetal Bovine Serum Inhibits Hepatitis C Virus Attachment to Host Cells","volume":"193","author":"Qin","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Schilling-Loeffler, K., Viera-Segura, O., Corman, V.M., Schneider, J., Gadicherla, A.K., Schotte, U., and Johne, R. (2021). Cell Culture Isolation and Whole Genome Characterization of Hepatitis e Virus Strains from Wild Boars in Germany. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112302"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"e2617","DOI":"10.1111\/tbed.14608","article-title":"Isolation of 15 Hepatitis E Virus Strains Lacking ORF1 Rearrangements from Wild Boar and Pig Organ Samples and Efficient Replication in Cell Culture","volume":"69","author":"Gremmel","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2012.01.001","article-title":"Evaluation of Methods Measuring the Capsid Integrity and\/or Functions of Noroviruses by Heat Inactivation","volume":"181","author":"Li","year":"2012","journal-title":"J. Virol. Methods"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Cuevas-ferrando, E., Randazzo, W., P\u00e9rez-catalu\u00f1a, A., Navarro, D., Martin-latin, S., D\u00edaz-reolid, A., Gir\u00f3n-guzm\u00e1n, I., Allende, A., and S\u00e1nchez, G. (2021). Viability RT-PCR for SARS-CoV-2: A Step Forward to Solve the Infectivity Quandary of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC.","DOI":"10.1101\/2021.03.22.21253818"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/12\/676\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:31:17Z","timestamp":1760131877000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/12\/676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,27]]},"references-count":89,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["vetsci10120676"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10120676","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,27]]}}}