{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T22:34:20Z","timestamp":1789425260497,"version":"build-2803163510"},"reference-count":73,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>After an extended period of detecting classical virulent, attenuated, and very virulent IBDV, a novel variant (nVarIBDV) was confirmed in Egypt in this study in 18, IBD vaccinated, chicken flocks aged 19\u201349 days. Partial sequence of viral protein 2 (VP2) [219 aa, 147\u2013366, resembling 657 bp] of two obtained isolates (nos. 3 and 4) revealed nVarIBDV (genotype A2d) and OR682618 and OR682619 GenBank accession numbers were obtained. Phylogenetic analysis revealed that both nVarIBDV isolates were closely related to nVarIBDV strains (A2d) circulating in China, exhibiting 100% identity to SD-2020 and 99.5\u201398.1% similarity to ZD-2018-1, QZ, GX and SG19 strains, respectively. Similarity to USA variant strains, belonging to genotypes A2b (9109), A2c (GLS) and A2a (variant E), respectively, was 95.5\u201392.6%. Also, the VP2 hypervariable region in those two, A2d, isolates revealed greater similarities to Faragher 52\/70 (Vaxxitek\u00ae) at 90.4% and to an Indian strain (Ventri-Plus\u00ae) and V217 (Xtreme\u00ae) at 89.7% and 86\u201388.9% in other vaccines. Histopathological examination of both the bursa of Fabricius and spleen collected from diseased chickens in flock no. 18 revealed severe atrophy. In conclusion, further studies are required to investigate the epidemiological situation of this novel genotype across the country, and to assess various vaccine protections against nVarIBDV. Additionally, vaccination of breeders with inactivated IBD vaccines including this nVarIBDV is essential to obtain specific maternal antibodies in their broilers.<\/jats:p>","DOI":"10.3390\/vetsci11020098","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T06:50:22Z","timestamp":1708498222000},"page":"98","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Isolation, Molecular, and Histopathological Patterns of a Novel Variant of Infectious Bursal Disease Virus in Chicken Flocks in Egypt"],"prefix":"10.3390","volume":"11","author":[{"given":"Ahmed H.","family":"Salaheldin","sequence":"first","affiliation":[{"name":"Department Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria 21944, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatem S.","family":"Abd El-Hamid","sequence":"additional","affiliation":[{"name":"Department of Bird and Rabbit Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9250-9255","authenticated-orcid":false,"given":"Hany F.","family":"Ellakany","sequence":"additional","affiliation":[{"name":"Department of Bird and Rabbit Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0995-7891","authenticated-orcid":false,"given":"Mostafa A.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine, Menoufia University, Shebeen Elkom 32511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1272-153X","authenticated-orcid":false,"given":"Ahmed R.","family":"Elbestawy","sequence":"additional","affiliation":[{"name":"Department of Bird and Rabbit Diseases, Faculty of Veterinary Medicine, Menoufia University, Shebeen Elkom 32511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"ref_1","unstructured":"David, E. 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