{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:36:22Z","timestamp":1788582982704,"version":"build-2803163510"},"reference-count":76,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The objective of this study was to investigate the effect of the designed herbal formula (DHF) on growth performance, blood indices, organ traits, and cecum microbiology in broilers. A total of 96 male broilers of 1 d were selected and randomly assigned to two groups with six replicates of eight broilers each. The control (CON) and the basal diet containing 1.0% DHF (Astragali radix, Atractylodes macrocephala Koidz., Isatis tinctoria Linnaeus, and Citri reticulatae pericarpium, 2:1:1:2) were fed separately. The experiment was conducted for 35 days. The results showed that the DHF diet increased body weight and decreased the feed conversion ratio (FCR) (p &lt; 0.05). At 21 days, the spleen, thymus, lymphocytes, and thrombocytes were increased (p &lt; 0.05), and pancreas, duodenum, heterophils, and mean corpuscular hemoglobin (MCH) were decreased (p &lt; 0.05). At 35 days, the heart, pancreas, white blood cell, heterophils, hemoglobin, MCH and mean corpuscular hemoglobin concentration (MCHC) were decreased, while lymphocytes and middle cells were increased (p &lt; 0.05). The results of microbial diversity analysis showed that the DHF diet decreased the microbial diversity of the cecum. Firmicutes and Bacteroidetes were the dominant phyla, where the DHF diet increased the relative abundances of Bacteroides uniformis, Bacteroides vulgatus, and Faecalibacterium prausnitzii, and then decreased the relative abundance of Shigella sonnei. In conclusion, DHF played a positive role in improving the growth performance, immune performance, and relative abundance of Bacteroides uniformis, Bacteroides vulgatus, and Faecalibacterium prausnitzii in cecum microbiology in broilers, and has the potential to be used as a novel feed additive.<\/jats:p>","DOI":"10.3390\/vetsci11030107","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T03:31:23Z","timestamp":1709263883000},"page":"107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Designed Herbal Formula on Growth Performance, Blood Indices, Organ Traits, and Cecum Microbiology in Broilers"],"prefix":"10.3390","volume":"11","author":[{"given":"Yuelong","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of CAAS, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of CAAS, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of CAAS, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of CAAS, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of CAAS, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"916473","DOI":"10.3389\/fvets.2022.916473","article-title":"Potential feed additives as antibiotic alternatives in broiler production","volume":"9","author":"Ayalew","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"692839","DOI":"10.3389\/fnut.2021.692839","article-title":"Progress on gut health maintenance and antibiotic alternatives in broiler chicken production","volume":"8","author":"Zhu","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chattopadhyay, M.K. (2014). Use of antibiotics as feed additives: A burning question. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00334"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Liu, Y., Wu, Y., Wu, J., Li, X., Yu, L., Xie, K., Zhang, M., Ren, L., Ji, Y., and Li, Y. (2022). Exposure to veterinary antibiotics via food chain disrupts gut microbiota and drives increased Escherichia coli virulence and drug resistance in young adults. Pathogens, 11.","DOI":"10.3390\/pathogens11091062"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Puva\u010da, N., Ljubojevi\u0107 Peli\u0107, D., Peli\u0107, M., Bursi\u0107, V., Tufarelli, V., Piemontese, L., and Vukovi\u0107, G. (2023). Microbial resistance to antibiotics and biofilm formation of bacterial isolates from different carp species and risk assessment for public health. Antibiotics, 12.","DOI":"10.3390\/antibiotics12010143"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102830","DOI":"10.1016\/j.psj.2023.102830","article-title":"Prevalence and antimicrobial resistance profile of bacterial pathogens isolated from poultry in Jiangxi Province, China from 2020 to 2022","volume":"102","author":"Tan","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_7","first-page":"189","article-title":"Effects of dietary supplementation of organic acids and phytase on performance and intestinal histomorphology of broilers","volume":"7","author":"Mohammadagheri","year":"2016","journal-title":"Vet. Res. Forum."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.vetpar.2012.02.017","article-title":"Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella","volume":"188","author":"Giannenas","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101266","DOI":"10.1016\/j.psj.2021.101266","article-title":"Dietary effect of licorice (Glycyrrhiza glabra) on quail performance, carcass, blood metabolites and intestinal microbiota","volume":"100","author":"Reda","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"473","DOI":"10.3389\/fvets.2019.00473","article-title":"Utility of feed enzymes and yeast derivatives in ameliorating deleterious effects of coccidiosis on intestinal health and function in broiler chickens","volume":"6","author":"Kiarie","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1080\/10495398.2022.2136677","article-title":"Chicken \u03b2-defensin-1 peptide as a candidate anticoccidial agent in broiler chickens","volume":"34","author":"Mahmoud","year":"2023","journal-title":"Anim. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s11250-019-02118-3","article-title":"Meta-analysis of the influence of phytobiotic (pepper) supplementation in broiler chicken performance","volume":"52","author":"Ogbuewu","year":"2020","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"112895","DOI":"10.1016\/j.jep.2020.112895","article-title":"Astragali radix (Huangqi): A promising edible immunomodulatory herbal medicine","volume":"258","author":"Chen","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.carbpol.2016.01.013","article-title":"The immune adjuvant response of polysaccharides from Atractylodis macrocephalae Koidz in chickens vaccinated against Newcastle disease (ND)","volume":"141","author":"Zhao","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"103032","DOI":"10.1016\/j.psj.2023.103032","article-title":"The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4\/NF-\u03baB signaling pathway","volume":"102","author":"Wang","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1080\/1828051X.2016.1222246","article-title":"Effect of dried Tangerine peel extract supplementation on the growth performance and antioxidant status of broiler chicks","volume":"15","author":"Jiang","year":"2016","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3298","DOI":"10.3382\/ps\/pez178","article-title":"A mixture of daidzein and Chinese herbs increases egg production and eggshell strength as well as blood plasma Ca, P, antioxidative enzymes, and luteinizing hormone levels in post-peak, brown laying hens","volume":"98","author":"Xiao","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1111\/jpn.12473","article-title":"Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens","volume":"100","author":"Li","year":"2016","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e15316","DOI":"10.7717\/peerj.15316","article-title":"The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese","volume":"11","author":"Fu","year":"2023","journal-title":"PeerJ"},{"key":"ref_20","first-page":"392743","article-title":"Hepatoprotective effects of a chinese herbal formula, longyin decoction, on carbon-tetrachloride-induced liver injury in chickens","volume":"2013","author":"Wang","year":"2013","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"108451","DOI":"10.1016\/j.exppara.2022.108451","article-title":"Effects of \u201cShi Ying Zi\u201d powder and osthole on immune and antioxidant function of Eimeria tenella-infected broilers","volume":"246","author":"Cheng","year":"2023","journal-title":"Exp. Parasitol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/japr\/3.1.101","article-title":"National Research Council Nutrient Requirements of Poultry-Ninth Revised Edition (1994)","volume":"3","author":"Dale","year":"1994","journal-title":"J. Appl. Poult. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101939","DOI":"10.1016\/j.psj.2022.101939","article-title":"Effects of fenugreek seed extracts on growth performance and intestinal health of broilers","volume":"101","author":"Yang","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_24","unstructured":"(2019). Operating Procedure of Livestock and Poultry Slaughtering\u2014Chicken. Standard No. GB\/T 194728-2018."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Rohart, F., Gautier, B., Singh, A., and Ka, L.C. (2017). mixOmics: An R package for \u2018omics feature selection and multiple data integration. PLoS Comput. Biol., 13.","DOI":"10.1101\/108597"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W.S., and Huttenhower, C. (2011). Metagenomic biomarker discovery and explanation. Genome Biol., 12.","DOI":"10.1186\/gb-2011-12-s1-p47"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., and Yuan, Z. (2014). Antibiotic alternatives: The substitution of antibiotics in animal husbandry?. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00217"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"417","DOI":"10.5483\/BMBRep.2022.55.9.102","article-title":"Systems pharmacology approaches in herbal medicine research: A brief review","volume":"55","author":"Lee","year":"2022","journal-title":"BMB Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liang, X., Yamazaki, K., Kamruzzaman, M., Bi, X., Panthee, A., and Sano, H. (2013). Effects of Chinese herbal medicine on plasma glucose, protein and energy metabolism in sheep. J. Anim. Sci. Biotechnol., 4.","DOI":"10.1186\/2049-1891-4-51"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.5194\/aab-62-33-2019","article-title":"The effect of herbal feed additive on the growth performance, carcass characteristics and meat quality of broiler chickens fed low-energy diets","volume":"62","author":"Antoszkiewicz","year":"2019","journal-title":"Arch. Anim. Breed."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Oloruntola, O.D., Agbede, J.O., Ayodele, S.O., and Oloruntola, D.A. (2019). Neem, pawpaw and bamboo leaf meal dietary supplementation in broiler chickens: Effect on performance and health status. J. Food Biochem., 43.","DOI":"10.1111\/jfbc.12723"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s11250-022-03274-9","article-title":"Impact of drinking water supplementation of a blend of peppermint, coneflower (Echinacea purpurea), thyme, propolis, and prebiotic on performance, serum constituents, and immunocompetence of broiler chickens","volume":"54","author":"Behboodi","year":"2022","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101684","DOI":"10.1016\/j.psj.2021.101684","article-title":"Hot red pepper powder as a safe alternative to antibiotics in organic poultry feed: An updated review","volume":"101","author":"Elbestawy","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3046","DOI":"10.1080\/10495398.2022.2130798","article-title":"The effects of peppermint (Mentha piperita L.) and chicory (Cichorium intybus L.) in comparison with a prebiotic on productive performance, blood constituents, immunity and intestinal microflora in broiler chickens","volume":"34","author":"Tufarelli","year":"2023","journal-title":"Anim. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"skad212","DOI":"10.1093\/jas\/skad212","article-title":"Dietary supplementation of Ocimum gratissimum improves growth performance and immune response in broilers under high ambient temperature","volume":"101","author":"Lin","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Alagawany, M., Elnesr, S.S., Farag, M.R., Abd El-Hack, M.E., Khafaga, A.F., Taha, A.E., Tiwari, R., Yatoo, M.I., Bhatt, P., and Marappan, G. (2019). Use of Licorice (Glycyrrhiza glabra) herb as a feed additive in poultry: Current knowledge and prospects. Animals, 9.","DOI":"10.3390\/ani9080536"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s13020-022-00633-4","article-title":"Inflammatory bowel disease: An overview of Chinese herbal medicine formula-based treatment","volume":"17","author":"Yuan","year":"2022","journal-title":"Chin. Med."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Liu, Y., Liang, S., Zi, X., Yan, S., Liu, M., Li, M., Zhao, Y., Dou, T., Ge, C., and Wang, K. (2022). Influence of Chinese herbal formula on bone characteristics of cobb broiler chickens. Genes, 13.","DOI":"10.3390\/genes13101865"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1002\/vms3.971","article-title":"Evaluation of therapeutic effects of an herbal mixture (Echinacea purpurea and Glycyrrhiza glabra) for treatment of clinical coccidiosis in broilers","volume":"9","author":"Ghafouri","year":"2023","journal-title":"Vet. Med. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"759456","DOI":"10.3389\/fvets.2021.759456","article-title":"Methodologies to assess the bioactivity of an herbal extract on immunity, health, welfare and production performance in the chicken: The case of Melissa officinalis L. extract","volume":"8","author":"Travel","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1515\/biol-2021-0031","article-title":"Supplementation of probiotics in water beneficial growth performance, carcass traits, immune function, and antioxidant capacity in broiler chickens","volume":"16","author":"Zhang","year":"2021","journal-title":"Open Life Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3409\/fb62_3.277","article-title":"Influence of synbiotics delivered in ovo on immune organs development and structure","volume":"62","author":"Siwek","year":"2014","journal-title":"Folia Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"183","DOI":"10.12688\/f1000research.51219.3","article-title":"The effects of lactic acid bacteria and yeasts as probiotics on the growth performance, relative organ weight, blood parameters, and immune responses of broiler: A meta-analysis","volume":"10","author":"Sjofjan","year":"2021","journal-title":"F1000Research"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/asj.13133","article-title":"Immunomodulatory effect of \u03b3-irradiated Astragalus polysaccharides on immunosuppressed broilers","volume":"90","author":"Li","year":"2019","journal-title":"Anim. Sci. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2790","DOI":"10.3382\/ps\/pez071","article-title":"Effect of the flavonoid baicalein as a feed additive on the growth performance, immunity, and antioxidant capacity of broiler chickens","volume":"98","author":"Zhou","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"705279","DOI":"10.3389\/fnut.2021.705279","article-title":"Effects of dietary ginsenoside Rg1 supplementation on growth performance, gut health, and serum immunity in broiler chickens","volume":"8","author":"Song","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102903","DOI":"10.1016\/j.psj.2023.102903","article-title":"Effects of ginger extract and\/or propolis extract on immune system parameters of vaccinated broilers","volume":"102","author":"Daha","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Diaz Carrasco, J.M., Casanova, N.A., and Fern\u00e1ndez Miyakawa, M.E. (2019). Microbiota, gut health and chicken productivity: What is the connection?. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100374"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wickramasuriya, S.S., Park, I., Lee, K., Lee, Y., Kim, W.H., Nam, H., and Lillehoj, H.S. (2022). Role of physiology, immunity, microbiota, and infectious diseases in the gut health of poultry. Vaccines, 10.","DOI":"10.3390\/vaccines10020172"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Alharthi, A.S., Alruwaili, N.W., Al-Baadani, H.H., Al-Garadi, M.A., Shamlan, G., and Alhidary, I.A. (2023). Investigating the effect of Pulicaria jaubertii as a natural feed additive on the growth performance, blood biochemistry, immunological response, and cecal microbiota of broiler chickens. Animals, 13.","DOI":"10.3390\/ani13061116"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e00600-20","DOI":"10.1128\/AEM.00600-20","article-title":"The gut microbiota of laying hens and its manipulation with prebiotics and probiotics to enhance gut health and food safety","volume":"86","author":"Khan","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1848158","DOI":"10.1080\/19490976.2020.1848158","article-title":"Gut Bacteroides species in health and disease","volume":"13","author":"Zafar","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"8787","DOI":"10.3748\/wjg.v21.i29.8787","article-title":"Role of the normal gut microbiota","volume":"21","author":"Jandhyala","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2358","DOI":"10.3382\/ps.2012-02935","article-title":"Broilers fed dietary vitamins harbor higher diversity of cecal bacteria and higher ratio of Clostridium, Faecalibacterium, and Lactobacillus than broilers with no dietary vitamins revealed by 16S rRNA gene clone libraries","volume":"92","author":"Luo","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Braz, V.S., Melchior, K., and Moreira, C.G. (2020). Escherichia coli as a multifaceted pathogenic and versatile bacterium. Front. Cell Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.548492"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Kim, J.S., Lee, M.S., and Kim, J.H. (2020). Recent updates on outbreaks of shiga toxin-producing Escherichia coli and its potential reservoirs. Front. Cell Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.00273"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e00060-18","DOI":"10.1128\/CMR.00060-18","article-title":"Escherichia coli pathobionts associated with inflammatory bowel disease","volume":"32","author":"Vallance","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"781934","DOI":"10.3389\/fimmu.2022.781934","article-title":"Effects of dietary Astragalus polysaccharide supplementation on the Th17\/Treg balance and the gut microbiota of broiler chickens challenged with necrotic enteritis","volume":"13","author":"Song","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13568-021-01190-z","article-title":"Dietary Purslane (Portulaca oleracea L.) promotes the growth performance of broilers by modulation of gut microbiota","volume":"11","author":"Wang","year":"2021","journal-title":"AMB Express"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1089\/dna.2021.0080","article-title":"Baicalin-aluminum modulates the broiler gut microbiome","volume":"40","author":"Guo","year":"2021","journal-title":"DNA Cell Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"828","DOI":"10.3382\/ps\/pey393","article-title":"Effects of glucose oxidase on growth performance, gut function, and cecal microbiota of broiler chickens","volume":"98","author":"Wu","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.aninu.2021.03.008","article-title":"Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health","volume":"7","author":"Wang","year":"2021","journal-title":"Anim. Nutr."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Grondin, J.M., D\u00e9jean, G., Van Petegem, F., and Brumer, H. (2022). Cell surface xyloglucan recognition and hydrolysis by the human gut commensal Bacteroides uniformis. Appl. Environ. Microbiol., 88.","DOI":"10.1128\/AEM.01566-21"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"871080","DOI":"10.3389\/fimmu.2022.871080","article-title":"Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet","volume":"13","author":"Roquim","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Vallianou, N.G., Kounatidis, D., Tsilingiris, D., Panagopoulos, F., Christodoulatos, G.S., Evangelopoulos, A., Karampela, I., and Dalamaga, M. (2023). The role of next-generation probiotics in obesity and obesity-associated disorders: Current knowledge and future perspectives. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076755"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.bpg.2017.09.011","article-title":"Action and function of Faecalibacterium prausnitzii in health and disease","volume":"31","author":"Faria","year":"2017","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"16731","DOI":"10.1073\/pnas.0804812105","article-title":"Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients","volume":"105","author":"Sokol","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"e00300-15","DOI":"10.1128\/mBio.00300-15","article-title":"Identification of metabolic signatures linked to anti-inflammatory effects of Faecalibacterium prausnitzii","volume":"6","author":"Miquel","year":"2015","journal-title":"mBio"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"De Cesare, A., Sala, C., Castellani, G., Astolfi, A., Indio, V., Giardini, A., and Manfreda, G. (2020). Effect of Lactobacillus acidophilus D2\/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0228338"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00203-020-02034-3","article-title":"Shigella sonnei: Virulence and antibiotic resistance","volume":"203","author":"Shad","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Anokwuru, C.P., Tankeu, S., van Vuuren, S., Viljoen, A., Ramaite, I.D.I., Taglialatela-Scafati, O., and Combrinck, S. (2020). Unravelling the antibacterial activity of Terminalia sericea Root bark through a metabolomic approach. 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