{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:09:13Z","timestamp":1791515353640,"version":"4.3.4"},"reference-count":74,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture, Food and Forestry Policies","award":["04250069681 TuBAvI-2"],"award-info":[{"award-number":["04250069681 TuBAvI-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Native breed conservation is an important component of poultry biodiversity. The aim of this work is to describe different steps that lead to donor selection for the implementation of the Italian Semen Cryobank of Autochthonous Chicken and Turkey Breeds. The variability within and between breeds was evaluated, and the stored semen reproductive capacity was in vivo tested using artificial insemination. Semen from Bionda Piemontese, Bianca di Saluzzo and Pepoi roosters was collected and processed. Concentration, volume, sperm membrane integrity, total motile sperm, progressive motile sperm and kinetic parameters were analyzed; sperm parameters accounting for bird variability were used to select male donors. Fresh semen quality parameters measured in donor ejaculates showed significant differences between breeds; no differences were found after cryopreservation. Variability in the fertilizing ability of cryopreserved semen was found within a breed (5\u201316%) and between birds within a breed (BP = 3\u20137%; BS = 7\u201331%; PP = 6\u201322%); only sperm quality parameters measured in fresh ejaculates, not frozen\/thawed, may be associated with in vivo fertility results. In conclusion, sperm concentration and progressive motility were successfully used as selection parameters to identify chicken male donors with improved sperm quality for sperm cryobanking. However, new reliable sperm markers to predict cryopreserved semen\u2019s fertilizing ability are required.<\/jats:p>","DOI":"10.3390\/vetsci11040148","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T12:41:57Z","timestamp":1711543317000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Selection of Male Donors in Local Chicken Breeds to Implement the Italian Semen Cryobank: Variability in Semen Quality, Freezability and Fertility"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1560-1768","authenticated-orcid":false,"given":"Manuela","family":"Madeddu","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0881-2332","authenticated-orcid":false,"given":"Luisa","family":"Zaniboni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8027-2193","authenticated-orcid":false,"given":"Stefano Paolo","family":"Marelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Tognoli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Belcredito","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9680-1264","authenticated-orcid":false,"given":"Nicolaia","family":"Iaffaldano","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Environmental and Food Science, University of Molise, 86100 Campobasso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7454-4970","authenticated-orcid":false,"given":"Michele","family":"Di Iorio","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Environmental and Food Science, University of Molise, 86100 Campobasso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5625-0357","authenticated-orcid":false,"given":"Silvia","family":"Cerolini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, University of Milan, via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"key":"ref_1","unstructured":"FAO (2020). 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