{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T19:32:09Z","timestamp":1773084729756,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Background: the present study aimed to investigate the immunological response to common vector-borne pathogens and to evaluate their impact on haematochemical parameters in owned dogs. Methods: Blood samples were collected from 400 clinically healthy dogs living in an endemic area (Sardinia Island, Italy). All dogs were serologically tested for VBDs and divided into groups based on their negative (Neg) or positive response towards Ehrlichia (Ehrl), Rickettsia (Rick), Leishmania (Leish), Borrelia (Borr), Anaplasma (Anapl), and Bartonella (Barto). A Kruskall\u2013Wallis\u2019s test, followed by Dunn\u2019s post hoc comparison test, was applied to determine the statistical effect of negativity and single or multiple positivity on the studied parameters. Results: the group of dogs simultaneously presenting antibodies towards Leishmania, Ehrlichia, and Rickettsia showed higher values of total proteins, globulins, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase, and amylase than dogs that tested negative or dogs with antibodies toward a single pathogen investigated herein. Conclusions: Our results seem to suggest that exposure to more vector-borne pathogens could lead to greater liver function impairment and a greater inflammatory state. Further investigations are needed in order to better clarify how co-infections affect haematochemical patterns in dogs living in endemic areas of VBDs.<\/jats:p>","DOI":"10.3390\/vetsci11050205","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T09:58:56Z","timestamp":1715162336000},"page":"205","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Haematochemical Profile of Healthy Dogs Seropositive for Single or Multiple Vector-Borne Pathogens"],"prefix":"10.3390","volume":"11","author":[{"given":"Raffaella","family":"Cocco","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, Teaching Veterinary Hospital, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8577-5678","authenticated-orcid":false,"given":"Sara","family":"Sechi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Teaching Veterinary Hospital, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4343-8937","authenticated-orcid":false,"given":"Maria","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Messina, Polo Universitario dell\u2019Annunziata, 98168 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Messina, Polo Universitario dell\u2019Annunziata, 98168 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6139-2691","authenticated-orcid":false,"given":"Francesca","family":"Arfuso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Messina, Polo Universitario dell\u2019Annunziata, 98168 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabetta","family":"Giudice","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Messina, Polo Universitario dell\u2019Annunziata, 98168 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.vetpar.2017.12.021","article-title":"Arthropod-borne pathogens of dogs and cats: From pathways and times of transmission to disease control","volume":"251","author":"Otranto","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"de la Fuente, J., Villar, M., Cabezas-Cruz, A., Estrada-Pe\u00f1a, A., Ayll\u00f3n, N., and Alberdi, P. (2016). Tick\u2013Host\u2013Pathogen Interactions: Conflict and Cooperation. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005488"},{"key":"ref_3","unstructured":"(2023, June 08). Control of Neglected Tropical Diseases. Available online: https:\/\/www.who.int\/teams\/control-of-neglected-tropical-diseases."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hotez, P.J. (2014). Neglected Parasitic Infections and Poverty in the United States. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0003012"},{"key":"ref_5","first-page":"5","article-title":"Vector-borne diseases\u2014Constant challenge for practicing veterinarians: Recommendations from the CVBD World Forum","volume":"20","author":"Baneth","year":"2012","journal-title":"Parasit. Vectors"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Klink, J.C., Rieger, A., Wohlsein, P., Siebert, U., and Obiegala, A. (2024). Vector-Borne and Zoonotic Pathogens in Raccoon Dogs (Nyctereutes procyonoides) and Raccoons (Procyon lotor) from Schleswig-Holstein, Germany. Pathogens, 13.","DOI":"10.3390\/pathogens13030270"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Giudice, E., Di Pietro, S., Alaimo, A., Blanda, V., Lelli, R., Francaviglia, F., Caracappa, S., and Torina, A. (2014). A molecular survey of Rickettsia felis in fleas from cats and dogs in Sicily (Southern Italy). PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0106820"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.pt.2017.05.013","article-title":"Zoonotic Parasites of Sheltered and Stray Dogs in the Era of the Global Economic and Political Crisis","volume":"33","author":"Otranto","year":"2017","journal-title":"Trends Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1126\/science.1239401","article-title":"Climate change and infectious diseases: From evidence to a predictive framework","volume":"341","author":"Altizer","year":"2013","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.1111\/gcb.14651","article-title":"Effects of historic and projected climate change on the range and impacts of an emerging wildlife disease","volume":"25","author":"Price","year":"2019","journal-title":"Glob. Chang. Biol."},{"key":"ref_11","first-page":"238","article-title":"Impact of Anthropogenic Environmental Alterations on Vector-Borne Diseases","volume":"10","author":"Vora","year":"2008","journal-title":"Medscape J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Diakou, A., Di Cesare, A., Morelli, S., Colombo, M., Halos, L., Simonato, G., Tamvakis, A., Beugnet, F., Paoletti, B., and Traversa, D. (2019). Endoparasites and vector-borne pathogens in dogs from Greek islands: Pathogen distribution and zoonotic implications. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007003"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1186\/s13071-020-04172-5","article-title":"Serological evaluation of selected vector-borne pathogens in owned dogs from northern Spain based on a multicenter study using a commercial test","volume":"13","author":"Roura","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Petruccelli, A., Ferrara, G., Iovane, G., Schettini, R., Ciarcia, R., Caputo, V., Pompameo, M., Pagnini, U., and Montagnaro, S. (2020). Seroprevalence of Ehrlichia spp., Anaplasma spp., Borrelia burgdorferi sensu lato, and Dirofilaria immitis in Stray Dogs, from 2016 to 2019, in Southern Italy. Animals, 11.","DOI":"10.3390\/ani11010009"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s00436-020-06973-0","article-title":"Seroprevalence and risk factors associated with Leishmania infantum in dogs in Sardinia (Italy), an endemic island for leishmaniasis","volume":"120","author":"Tamponi","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Chisu, V., Dei Giudici, S., Foxi, C., Chessa, G., Peralta, F., Sini, V., and Masala, G. (2023). Anaplasma Species in Ticks Infesting Mammals of Sardinia, Italy. Animals, 13.","DOI":"10.3390\/ani13081332"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.1128\/jcm.29.12.2741-2745.1991","article-title":"Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis","volume":"29","author":"Dawson","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1749-6632.2003.tb07350.x","article-title":"Ehrlichiosis and rickettsiosis in a canine population of Northern Sardinia","volume":"990","author":"Cocco","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.2460\/ajvr.1990.51.08.1312","article-title":"Kinetics of IgM and IgG responses to experimental and naturally-occurring Rickettsia rickettsii infection in dogs","volume":"51","author":"Breitschwerdt","year":"1990","journal-title":"Am. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0195-5616(91)50002-4","article-title":"Rocky Mountain spotted fever","volume":"21","author":"Comer","year":"1991","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_21","unstructured":"(2018). Manual of diagnostic tests and vaccines for terrestrial animals. Off. Int. Epizooties, 3, 11."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Foglia Manzillo, V., Gizzarelli, M., Vitale, F., Montagnaro, S., Torina, A., Sotera, S., and Oliva, G. (2018). Serological and entomological survey of canine leishmaniasis in Lampedusa island, Italy. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1606-x"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1297-9716-43-15","article-title":"Strategies of exploitation of mammalian reservoirs by Bartonella species","volume":"43","author":"Deng","year":"2012","journal-title":"Vet. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.cvsm.2010.03.010","article-title":"Infective Endocarditis in Dogs: Diagnosis and Therapy","volume":"40","author":"MacDonald","year":"2010","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.micpath.2017.10.024","article-title":"Changes in serum proteins in dogs with Ehrlichia canis infection","volume":"113","author":"Escribano","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.tvjl.2019.01.015","article-title":"An update on the treatment of canine monocytic ehrlichiosis (Ehrlichia canis)","volume":"46","author":"Mylonakis","year":"2019","journal-title":"Vet. J."},{"key":"ref_27","unstructured":"Meyer, D.J., and Harvey, J.W. (2004). Veterinary Laboratory Medicine: Interpretation & Diagnosis: Interpretation and Diagnosis, W. B. Saunders Co. [3rd ed.]."},{"key":"ref_28","first-page":"358","article-title":"Leishmaniasis: Clinical and diagnostic aspects","volume":"25","author":"Ciaramella","year":"2003","journal-title":"Compendium"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1756-3305-4-86","article-title":"LeishVet guidelines for the practical management of canine leishmaniosis","volume":"4","author":"Koutinas","year":"2011","journal-title":"Parasit. Vectors"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1136\/vr.141.10.247","article-title":"Clinical manifestations of infectious canine cyclic thrombocytopenia","volume":"141","author":"Harrus","year":"1997","journal-title":"Vet. Rec."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13071-015-0649-0","article-title":"Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe","volume":"8","author":"Sainz","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.vetmic.2010.10.005","article-title":"Anaplasmosis in dogs: The relation of haematological, biochemical and clinical alterations to antibody titre and PCR confirmed infection","volume":"149","author":"Ravnik","year":"2011","journal-title":"Vet. Microbiol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/5\/205\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:42:01Z","timestamp":1760107321000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/5\/205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,8]]},"references-count":32,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["vetsci11050205"],"URL":"https:\/\/doi.org\/10.3390\/vetsci11050205","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,8]]}}}