{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:14:30Z","timestamp":1760145270836,"version":"build-2065373602"},"reference-count":16,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jilin Province Science and Technology Development Plan Project","award":["20210204115YY","20210203180SF","GJ202211439020"],"award-info":[{"award-number":["20210204115YY","20210203180SF","GJ202211439020"]}]},{"name":"innovation and entrepreneurship project for college students of Jilin province","award":["20210204115YY","20210203180SF","GJ202211439020"],"award-info":[{"award-number":["20210204115YY","20210203180SF","GJ202211439020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>To establish a rapid real-time RT-PCR method for differentiating wild-type classical swine fever virus (CSFV) strains from vaccine strains (HCLV), we designed a universal primer targeting the NS3 gene to detect wild-type CSFV strains and vaccine strains simultaneously, and two TaqMan-MGB probes were designed to differentiate between wild-type and vaccine strains. After optimizing the RT-qPCR conditions, a rapid dual TaqMan-MGB RT-qPCR method for the detection and identification of CSFV and HCLV was developed. The results showed that method could specifically detect CSFV and HCLV with no cross-reactivity with other swine pathogens. The analytic sensitivity for the NS3 gene of CSFV and HCLV were 1.67 \u00d7 101 copies\/\u03bcL, respectively. For precision testing, the repeatability and reproducibility of the test was less than 2%. This method was successfully used for the rapid detection of 193 biological samples collected from CSFV-vaccinated pigs. This fast and accurate detection technology can be used for the detection of CSFV and is suitable for differentiating between wild-type CSFV strains and vaccine strains.<\/jats:p>","DOI":"10.3390\/vetsci11070289","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T06:51:53Z","timestamp":1719557513000},"page":"289","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Differential Detection of Wild-Type Classical Swine Fever Virus and Hog Cholera Lapinized Virus Vaccines by TaqMan MGB-Based Dual One-Step Real-Time RT-PCR"],"prefix":"10.3390","volume":"11","author":[{"given":"Yongzhe","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiqi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun 130033, China"},{"name":"Research and Development Center, Sairuisi Biotechnology (Jilin) Co., Ltd., Changchun 130102, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyu","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"},{"name":"Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun 130033, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songsong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongri","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Blome, S., Staubach, C., Henke, J., Carlson, J., and Beer, M. (2017). Classical Swine Fever-An Updated Review. Viruses, 9.","DOI":"10.3390\/v9040086"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.virusres.2014.12.006","article-title":"Studying classical swine fever virus: Making the best of a bad virus","volume":"197","author":"Ji","year":"2015","journal-title":"Virus Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1099\/vir.0.80238-0","article-title":"Genetic and antigenic characterization of an atypical pestivirus isolate, a putative member of a novel pestivirus species","volume":"85","author":"Schirrmeier","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Moennig, V. (2015). The control of classical swine fever in wild boar. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.01211"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/S0042-6822(03)00192-2","article-title":"Genetic and antigenic characterization of novel pestivirus genotypes: Implications for classification","volume":"311","author":"Becher","year":"2003","journal-title":"Virology"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Brown, V.R., and Bevins, S.N. (2018). A Review of Classical Swine Fever Virus and Routes of Introduction into the United States and the Potential for Virus Establishment. Front. Vet. Sci., 5.","DOI":"10.3389\/fvets.2018.00031"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"198151","DOI":"10.1016\/j.virusres.2020.198151","article-title":"Classical swine fever virus: The past, present and future","volume":"289","author":"Ganges","year":"2020","journal-title":"Virus Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/1297-9716-43-50","article-title":"Improved strategy for phylogenetic analysis of classical swine fever virus based on full-length E2 encoding sequences","volume":"43","author":"Postel","year":"2012","journal-title":"Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhou, B. (2019). Classical Swine Fever in China-An Update Minireview. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00187"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Fan, J., Liao, Y., Zhang, M., Liu, C., Li, Z., Li, Y., Li, X., Wu, K., Yi, L., and Ding, H. (2021). Anti-Classical Swine Fever Virus Strategies. Microorganisms, 9.","DOI":"10.3390\/microorganisms9040761"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.jviromet.2011.05.003","article-title":"A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever","volume":"175","author":"Liu","year":"2011","journal-title":"J. Virol. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jviromet.2009.03.003","article-title":"Two real-time RT-PCR assays of classical swine fever virus, developed for the genetic differentiation of naturally infected from vaccinated wild boars","volume":"159","author":"Liu","year":"2009","journal-title":"J. Virol. Methods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jviromet.2007.09.017","article-title":"Real-time RT-PCR assay for rapid and specific detection of classical swine fever virus: Comparison of SYBR Green and TaqMan MGB detection methods using novel MGB probes","volume":"147","author":"Grom","year":"2008","journal-title":"J. Virol. Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jviromet.2009.04.019","article-title":"Development of a primer-probe energy transfer real-time PCR assay for improved detection of classical swine fever virus","volume":"160","author":"Liu","year":"2009","journal-title":"J. Virol. Methods"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"171235","DOI":"10.1155\/2014\/171235","article-title":"Validation of a real time PCR for classical Swine Fever diagnosis","volume":"2014","author":"Dias","year":"2014","journal-title":"Vet. Med. Int."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Haines, F.J., Hofmann, M.A., King, D.P., Drew, T.W., and Crooke, H.R. (2013). Development and validation of a multiplex, real-time RT PCR assay for the simultaneous detection of classical and African swine fever viruses. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071019"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/7\/289\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:06:50Z","timestamp":1760108810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/7\/289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,28]]},"references-count":16,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["vetsci11070289"],"URL":"https:\/\/doi.org\/10.3390\/vetsci11070289","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2024,6,28]]}}}