{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:39:50Z","timestamp":1787762390289,"version":"build-2784847793"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T00:00:00Z","timestamp":1726876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture and Forestry, International Center for Livestock Research and Training","award":["E-373878\/522021"],"award-info":[{"award-number":["E-373878\/522021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>ProAKAP4 is a sperm structural protein that regulates motility through the PKA-dependent cAMP signaling pathway, which is synthesized as an X chromosome-linked member of the gene family. This study aims to determine the optimal level of proAKAP4 for evaluating sexed semen through investigating its relationship with the longevity of sperm quality in sexed Holstein bull sperm. A total of 30 sexed sperm samples (bearing X chromosomes) from 30 distinct Holstein bulls (n = 30) were analyzed. The frozen bull sperm samples were assessed for their proAKAP4 levels, mitochondrial membrane potential, plasma membrane and acrosome integrity (PMAI), and spermatozoa movement parameters at hours 0 and 3 after thawing. The proAKAP4 levels in the sexed sperm samples ranged from 16.35 to 72.10 ng\/10 M spz, with an average of 37.18 \u00b1 15.1 ng\/10 M spz. A strong positive correlation was observed between proAKAP4 levels and total motility, progressive motility, PMAI, high mitochondrial membrane potential, VAP, and VCL values after 3 h of incubation, when compared to post-thaw analyses. The results also reveal that spermatozoa with proAKAP4 levels of \u226540 ng\/10 M spz exhibit higher quality. In conclusion, the level of proAKAP4 in sexed sperm aligns with previous studies and shows potential as a biomarker for assessing the longevity of sexed sperm quality.<\/jats:p>","DOI":"10.3390\/vetsci11090444","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T08:54:17Z","timestamp":1727168057000},"page":"444","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8155-1960","authenticated-orcid":false,"given":"\u0130lktan","family":"Bastan","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University,15000 Burdur, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u0131rat","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University,15000 Burdur, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derya","family":"\u015eahin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, International Center for Livestock Research and Training, 06852 Ankara, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seher","family":"\u015eim\u015fek","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Quarantine, General Directorate of Food and Control, 06800 Ankara, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ufuk","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"s97","DOI":"10.1017\/S1751731118000721","article-title":"Applications and benefits of sexed semen in dairy and beef herds","volume":"12","author":"Holden","year":"2018","journal-title":"Animal"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.theriogenology.2008.01.006","article-title":"History of commercializing sexed semen for cattle","volume":"69","author":"Garner","year":"2008","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"s85","DOI":"10.1017\/S1751731118000496","article-title":"Semen sexing\u2013current state of the art with emphasis on bovine species","volume":"12","author":"Vishwanath","year":"2018","journal-title":"Animal"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.2478\/aoas-2022-0046","article-title":"Relationship between quality parameters and fertilizing ability of cryopreserved sexed bull sperm","volume":"22","author":"Gogol","year":"2022","journal-title":"Ann. 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