{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:11Z","timestamp":1792790171370,"version":"4.5.0"},"reference-count":91,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ARIMNET2","award":["FP7-ERANET-2013"],"award-info":[{"award-number":["FP7-ERANET-2013"]}]},{"name":"ARIMNET2","award":["618127"],"award-info":[{"award-number":["618127"]}]},{"name":"Acronym PeRFORM","award":["FP7-ERANET-2013"],"award-info":[{"award-number":["FP7-ERANET-2013"]}]},{"name":"Acronym PeRFORM","award":["618127"],"award-info":[{"award-number":["618127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The effect of four extenders on buck semen quality parameters was examined during a 48 h liquid storage. Semen was collected from six Skopelos bucks and diluted in the following extenders, containing: soy lecithin (SL, OviXcell\u00ae), plant phospholipids (PP, AndroMed\u00ae), egg yolk lecithin (EY, Steridyl\u00ae), or no phospholipids (basic extender). Samples were stored at 5 \u00b0C for 48 h and assessed at 0, 24 and 48 h for viability (eosin-nigrosin), acrosome integrity (SpermBlue\u00ae), membrane functional integrity (HOST), mitochondrial function (Rhodamine 123\/SYBR-14\/PI) and motility parameters (CASA). No significant reduction in total or progressive spermatozoa motility and mitochondrial function was observed at 24 h, whereas they all dropped significantly at 48 h, in all extenders. Spermatozoa viability, cell membrane functionality and acrosome integrity dropped progressively (0 h &gt; 24 h &gt; 48 h) in all groups. No significant difference among extenders was observed concerning spermatozoa mitochondrial function. Overall, spermatozoa viability, cell membrane functionality and acrosome integrity were higher in the three commercial extenders, compared to the basic extender. SL and EY extenders (OviXcell\u00ae and Steridyl\u00ae, respectively) preserved viability more effectively than the PP extender (AndroMed\u00ae). Total motility was higher in the PP extender, compared to the SL extender. Spermatozoa acrosome integrity tended to be higher in the EY extender compared to all the other extenders. Further investigation of the protective potential of different types of cryoprotectants on liquid buck semen storage is important.<\/jats:p>","DOI":"10.3390\/vetsci11100494","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T03:53:47Z","timestamp":1728618827000},"page":"494","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Three Commercially Available Extenders Containing Phospholipids of Different Sources on Skopelos Buck Liquid-Stored Sperm Quality"],"prefix":"10.3390","volume":"11","author":[{"given":"Aikaterini","family":"Saratsi","sequence":"first","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization-DIMITRA, ELGO Campus, Thermi, 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-4219","authenticated-orcid":false,"given":"Foteini","family":"Samartzi","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization-DIMITRA, ELGO Campus, Thermi, 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Tsiokos","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Science, Hellenic Agricultural Organization-DIMITRA, Paralimni, 58100 Giannitsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterini K.","family":"Theodosiadou","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology, Faculty of Veterinary Science, University of Thessaly, Trikalon 224, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Panagiotidis","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction & Artificial Insemination, Directorate of Veterinary Center of Thessaloniki, Ministry of Rural Development and Food, 9 Verias Str., 57008 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4980-3026","authenticated-orcid":false,"given":"Christina","family":"Ligda","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization-DIMITRA, ELGO Campus, Thermi, 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1602-6956","authenticated-orcid":false,"given":"Constantinos A.","family":"Rekkas","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization-DIMITRA, ELGO Campus, Thermi, 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.anireprosci.2012.01.014","article-title":"Semen Processing and Artificial Insemination in Health Management of Small Ruminants","volume":"130","author":"Cseh","year":"2012","journal-title":"Anim. 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