{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:08:38Z","timestamp":1765357718904,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"],"award-info":[{"award-number":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"]}]},{"name":"Key R&amp;D Project of Sichuan Provincial Science and Technology Department","award":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"],"award-info":[{"award-number":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"]}]},{"name":"Project from Sichuan Center for Rural Development Research","award":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"],"award-info":[{"award-number":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"],"award-info":[{"award-number":["32473109","2024YFFK0146","CR2225","NSF2021ZR01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Immunosuppression undermines intestinal barrier integrity. Cow placenta extract (CPE) primarily consists of active peptides with immunomodulatory and antioxidant effects. This study aimed to examine the preventive effect of CPE against intestinal damage induced by cyclophosphamide (Cy) in immunosuppressed mice. Thirty-six mice were randomly allocated into three groups: control group (C), model group (M), and treatment group (CPE). The mice in the CPE group were provided with 1500 mg\/kg\/day of CPE via gavage. In the last 3 days, mice in the groups M and CPE received intraperitoneal injections of 80 mg\/kg\/day of Cy. The results showed that CPE improved intestinal barrier function by decreasing serum d-Lactate (D-LA) levels and diamine oxidase (DAO) activity, while elevating the relative expression of Occludin, zonula occludens-1 (ZO-1), and mucin-2 (MUC-2) mRNA. Additionally, CPE improved the immune organ index and elevated the levels of secretory immunoglobulin A (sIgA), superoxide dismutase (SOD), interleukin-1beta (IL-1\u03b2), interleukin-4 (IL-4), interleukin-10 (IL-10), and tumor necrosis factor-\u03b1 (TNF-\u03b1) in the intestine, thereby enhancing intestinal mucosal immune function. Furthermore, CPE improved the diversity of intestinal microbiota and increased the abundance of Candidatus_Saccharimonas, Psychrobacter, and Enterorhabdus, which promoted the proper functioning of the intestines. These findings suggest that CPE effectively ameliorates Cy-induced intestinal damage by enhancing the intestinal barrier, improving immune function, and restoring intestinal microbiota.<\/jats:p>","DOI":"10.3390\/vetsci11100505","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T07:28:35Z","timestamp":1729495715000},"page":"505","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cow Placenta Extract Ameliorates Cyclophosphamide-Induced Intestinal Damage by Enhancing the Intestinal Barrier, Improving Immune Function, and Restoring Intestinal Microbiota"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9653-3973","authenticated-orcid":false,"given":"Yuquan","family":"Zhao","sequence":"first","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeru","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anguo","family":"Tang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijian","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Luo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1816-9328","authenticated-orcid":false,"given":"Yixin","family":"Huang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyi","family":"Dai","sequence":"additional","affiliation":[{"name":"Party School of the Communist Party of China Yaan Municipal Committee, Yaan 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Fan","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5806","authenticated-orcid":false,"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"G171","DOI":"10.1152\/ajpgi.00048.2015","article-title":"Homeostasis of the gut barrier and potential biomarkers","volume":"312","author":"Wells","year":"2017","journal-title":"Am. 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