{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:29:27Z","timestamp":1760146167340,"version":"build-2065373602"},"reference-count":11,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Surgical resection of subcutaneous neoplasms with clear margins is crucial for preventing local recurrence and avoiding adjuvant treatments. However, the evaluation of surgical margins often differs significantly from the histopathological assessment due to tissue shrinkage, which can result in inaccurate therapeutic assessments and unreliable patient prognoses. In this study, ten feline cadavers were utilized. Six 50 mm diameter specimens were collected from three regions (thorax, flank, femur) and measured at three time points: T0 (excision time), T1 (10 min after incision), and T2 (at least 48 h after sample collection and formalin fixation). Samples in the study group were stretched and fixed on a cork plate with pinpoint needles after excision to restore their original dimensions. All specimens exhibited a similar trend. After 48 h of formalin fixation, the control specimens showed significant shrinkage, with a reduction of 25.73% in radius and 26.32% in diameter. In contrast, the study specimens demonstrated minimal changes, with a radius reduction of \u22120.28% and no change in diameter. The results indicate that all feline skin specimens experienced significant shrinkage of approximately one-quarter from their pre-incisional size. Stretching and pinning the excised tissues allowed for the restoration and maintenance of original dimensions even after formalin fixation. This technique represents a valid and practical approach to minimize tissue shrinkage.<\/jats:p>","DOI":"10.3390\/vetsci11100507","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T07:28:35Z","timestamp":1729495715000},"page":"507","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-Shrinkage Technique for Feline Skin Samples"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6557-6899","authenticated-orcid":false,"given":"Caterina","family":"Kiniger","sequence":"first","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligita","family":"Zorgevica-Pockevica","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Vincenti","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.2460\/ajvr.77.9.1036","article-title":"Evaluation of the effects of anatomic location, histologic processing, and sample size on shrinkage of skin samples obtained from canine cadavers","volume":"77","author":"Reagan","year":"2016","journal-title":"Am. J. Vet. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.2460\/ajvr.2005.66.500","article-title":"Evaluation of the effect of routine histologic processing on the size of skin samples obtained from dogs","volume":"66","author":"Reimer","year":"2005","journal-title":"Am. J. Vet. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/vsu.12481","article-title":"The Effect of Specimen Preparation on Post-Excision and Post-Fixation Dimensions, Translation, and Distortion of Canine Cadaver Skin-Muscle-Fascia Specimens","volume":"45","author":"Risselada","year":"2016","journal-title":"Vet. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"939","DOI":"10.2460\/ajvr.76.11.939","article-title":"Effect of histologic processing on dimensions of skin samples obtained from cat cadavers","volume":"76","author":"Jeyakumar","year":"2015","journal-title":"Am. J. Vet. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1177\/1098612X15602951","article-title":"Effect of feline skin specimen preparation on postexcision and postfixation tissue shrinkage","volume":"18","author":"Risselada","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e307","DOI":"10.7717\/peerj.307","article-title":"Evaluation of the effect of formalin fixation on skin specimens in dogs and cats","volume":"2","author":"Miller","year":"2014","journal-title":"PeerJ"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1111\/ans.17109","article-title":"Skin tumour specimen shrinkage with excision and formalin fixation-how much and why: A prospective study and discussion of the literature","volume":"91","year":"2021","journal-title":"ANZ J. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1111\/j.1365-2133.2008.08994.x","article-title":"Shrinkage of skin excision specimens: Formalin fixation is not the culprit","volume":"160","author":"Dauendorffer","year":"2009","journal-title":"Br. J. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.2460\/ajvr.75.11.1004","article-title":"Effects of sample site and size, skin tension lines, surgeon, and formalin fixation on shrinkage of skin samples excised from canine cadavers","volume":"75","author":"Upchurch","year":"2014","journal-title":"Am. J. Vet. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"184","DOI":"10.2460\/javma.247.2.184","article-title":"Surgically planned versus histologically measured lateral tumor margins for resection of cutaneous and subcutaneous mast cell tumors in dogs: 46 cases (2010\u20132013)","volume":"247","author":"Risselada","year":"2015","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1177\/10935266231162684","article-title":"Tissue Shrinkage of Resected Specimens in Hirschsprung\u2019s Disease: Why Pediatric Surgeons Think the Bowel Specimen was Longer Than Indicated in the Pathology Report","volume":"26","author":"Gretser","year":"2023","journal-title":"Pediatr. Dev. Pathol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/10\/507\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:13:23Z","timestamp":1760112803000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/11\/10\/507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":11,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["vetsci11100507"],"URL":"https:\/\/doi.org\/10.3390\/vetsci11100507","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2024,10,15]]}}}