{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T13:36:06Z","timestamp":1784727366660,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["31702120"],"award-info":[{"award-number":["31702120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["KJQN201831"],"award-info":[{"award-number":["KJQN201831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities of China","doi-asserted-by":"publisher","award":["31702120"],"award-info":[{"award-number":["31702120"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities of China","doi-asserted-by":"publisher","award":["KJQN201831"],"award-info":[{"award-number":["KJQN201831"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study aimed to evaluate the influence of back-fat thickness (BF), at mating of sows, on autophagy in placenta and the potential mechanism. The sows were divided into two groups according to their BF at mating: BFI (15\u201320 mm, n = 14) and BFII (21\u201327 mm, n = 14) as the maternal obesity group. The placental samples used for investigating autophagic function and fatty acid profiles were obtained by vaginal delivery. Our results demonstrated that autophagy defects were observed in placenta from BFII sows along with altered circulating and placental fatty acid profiles. Indicative of impaired autophagy, reduced autophagic vesicles as well as LC3-positive puncta were linked to decreased mRNA or protein expression of autophagy-related genes, including ATG5, ATG7, Beclin1, ATG12, LC3, LAMP1 and LAMP2 in the placenta of BFII sows (p &lt; 0.05). Meanwhile, we found reduced conversion of LC3-I to LC3-II and up-regulated protein content of p62 in the placenta from BFII group (p &lt; 0.05). Furthermore, excessive back-fat was also associated with increased activation of AKT\/mTOR signaling and decreased mRNA content of transcription factors regulating the autophagic pathway, including PPAR\u03b1 and PGC1\u03b1, but increased mRNA expression of NcoR1 in placenta. Together, these findings indicate that maternal obesity incites autophagy injury in pig term placenta, which may contribute to augmented placental lipid accumulation and therefore impaired placental function.<\/jats:p>","DOI":"10.3390\/vetsci12020097","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T08:57:48Z","timestamp":1738054668000},"page":"97","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1191-1569","authenticated-orcid":false,"given":"Liang","family":"Tian","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Su","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyi","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyue","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1111\/jog.14315","article-title":"Maternal obesity reduces placental autophagy marker expression in uncomplicated pregnancies","volume":"46","author":"Cohen","year":"2020","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.placenta.2011.03.005","article-title":"Autophagy in term normal human placentas","volume":"32","author":"Signorelli","year":"2011","journal-title":"Placenta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.bbrc.2009.03.039","article-title":"The MAP1-LC3 conjugation system is involved in lipid droplet formation","volume":"382","author":"Shibata","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1038\/nature07976","article-title":"Autophagy regulates lipid metabolism","volume":"458","author":"Singh","year":"2009","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.cmet.2010.04.005","article-title":"Defective hepatic autophagy in obesity promotes ER stress and causes insulin resistance","volume":"11","author":"Yang","year":"2010","journal-title":"Cell. Metab."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/nm.3014","article-title":"Autophagy deficiency leads to protection from obesity and insulin resistance by inducing Fgf21 as a mitokine","volume":"19","author":"Kim","year":"2013","journal-title":"Nat. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.2337\/db14-0267","article-title":"Adiponectin stimulates autophagy and reduces oxidative stress to enhance insulin sensitivity during high-fat diet feeding in mice","volume":"64","author":"Liu","year":"2015","journal-title":"Diabetes"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.placenta.2014.01.003","article-title":"Maternal obesity is associated with a lipotoxic placental environment","volume":"35","author":"Saben","year":"2014","journal-title":"Placenta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/jas\/skx041","article-title":"Excessive backfat of sows at 109 d of gestation induces lipotoxic placental environment and is associated with declining reproductive performance","volume":"96","author":"Zhou","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1053\/plac.2002.0791","article-title":"Placental regulation of fatty acid delivery and its effect on fetal growth\u2014A review","volume":"23","author":"Haggarty","year":"2002","journal-title":"Placenta"},{"key":"ref_11","first-page":"59","article-title":"Maternal lipid metabolism and placental lipid transfer","volume":"65","author":"Herrera","year":"2006","journal-title":"Horm. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"E1","DOI":"10.1152\/ajpendo.00076.2013","article-title":"Early growth response protein-1 mediates lipotoxicity-associated placental inflammation: Role in maternal obesity","volume":"305","author":"Saben","year":"2013","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Li, J.W., Hu, J., Wei, M., Guo, Y.Y., and Yan, P.S. (2019). The Effects of Maternal Obesity on Porcine Placental Efficiency and Proteome. Animals, 9.","DOI":"10.3390\/ani9080546"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.anireprosci.2010.05.013","article-title":"Reproductive performance of gilts according to growth rate and backfat thickness at mating","volume":"121","author":"Bernardi","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.livsci.2019.02.012","article-title":"Excessive backfat of sows at mating promotes oxidative stress and up-regulates mitochondrial-mediated apoptotic pathway in the full-term placenta","volume":"222","author":"Tian","year":"2019","journal-title":"Livest. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.5713\/ab.20.0750","article-title":"Effects of prolonged photoperiod on growth performance, serum lipids and meat quality of Jinjiang cattle in winter","volume":"34","author":"Yu","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Yang, K., Cai, J., Pan, M., Sun, Q., and Sun, C. (2020). Mark4 Inhibited the Browning of White Adipose Tissue by Promoting Adipocytes Autophagy in Mice. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082752"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7376","DOI":"10.1002\/jcp.30408","article-title":"AMPK\/mTOR downregulated autophagy enhances aberrant endometrial decidualization in folate-deficient pregnant mice","volume":"236","author":"Zhang","year":"2021","journal-title":"J. Cell. Physiol."},{"key":"ref_19","first-page":"402","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u25b3\u25b3Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods Cell Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1002\/oby.21081","article-title":"Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic\/lysosomal proteolytic flux","volume":"23","author":"Bollinger","year":"2015","journal-title":"Obesity"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1242\/jcs.01131","article-title":"LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation","volume":"117","author":"Kabeya","year":"2004","journal-title":"J. Cell. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ymeth.2014.11.021","article-title":"LC3- and p62-based biochemical methods for the analysis of autophagy progression in mammalian cells","volume":"75","author":"Jiang","year":"2015","journal-title":"Methods"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sinha, R.A., Rajak, S., Singh, B.K., and Yen, P.M. (2020). Hepatic Lipid Catabolism via PPAR\u03b1-Lysosomal Crosstalk. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072391"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19860","DOI":"10.1073\/pnas.0906048106","article-title":"Adipose-specific deletion of autophagy-related gene 7 (atg7) in mice reveals a role in adipogenesis","volume":"106","author":"Zhang","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1530\/REP-09-0568","article-title":"The adverse effects of obesity on conception and implantation","volume":"140","author":"Brewer","year":"2010","journal-title":"Reproduction"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1042\/CS20090640","article-title":"Lipotoxicity in obese pregnancy and its potential role in adverse pregnancy outcome and obesity in the offspring","volume":"119","author":"Jarvie","year":"2010","journal-title":"Clin. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Jones, M.L., Mark, P.J., Mori, T.A., Keelan, J.A., and Waddell, B.J. (2013). Maternal dietary omega-3 fatty acid supplementation reduces placental oxidative stress and increases fetal and placental growth in the rat. Biol. Reprod., 88.","DOI":"10.1095\/biolreprod.112.103754"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod.111.098095","article-title":"Modulation of fatty acid transport and metabolism by maternal obesity in the human full-term placenta","volume":"87","author":"Dube","year":"2012","journal-title":"Biol. Reprod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.placenta.2013.03.009","article-title":"Placental oleic acid uptake is lower in male offspring of obese women","volume":"34","author":"Brass","year":"2013","journal-title":"Placenta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jri.2017.12.001","article-title":"Unsaturated fatty acids protect trophoblast cells from saturated fatty acid-induced autophagy defects","volume":"125","author":"Hong","year":"2018","journal-title":"J. Reprod. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Avagliano, L., Virgili, E., Gar\u00f2, C., Quadrelli, F., Doi, P., Samaja, M., Bulfamante, G.P., and Marconi, A.M. (2013). Autophagy and human parturition: Evaluation of LC3 expression in placenta from spontaneous or medically induced onset of labor. Biomed. Res. Int., 2013.","DOI":"10.1155\/2013\/689768"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Singh, R., and Cuervo, A.M. (2012). Lipophagy: Connecting autophagy and lipid metabolism. Int. J. Cell. Biol., 2012.","DOI":"10.1155\/2012\/282041"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/S1872-2075(08)60068-0","article-title":"Effects of leptin on porcine primary adiocytes lipolysis and mRNA expression of key lipolytic enzymes","volume":"24","author":"Li","year":"2008","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"647.e1","DOI":"10.1016\/j.ajog.2015.02.026","article-title":"Identification of early transcriptome signatures in placenta exposed to insulin and obesity","volume":"212","author":"Lassance","year":"2015","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/cr.2013.166","article-title":"Autophagy regulation by nutrient signaling","volume":"24","author":"Russell","year":"2014","journal-title":"Cell. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ceb.2009.11.005","article-title":"TOR-dependent control of autophagy: Biting the hand that feeds","volume":"22","author":"Neufeld","year":"2010","journal-title":"Curr. Opin. Cell. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nature13961","article-title":"Nutrient-sensing nuclear receptors coordinate autophagy","volume":"516","author":"Lee","year":"2014","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4612306","DOI":"10.1155\/2016\/4612306","article-title":"Peroxisome Proliferator-Activated Receptors in Female Reproduction and Fertility","volume":"2016","author":"Vitti","year":"2016","journal-title":"PPAR Res."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/97\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:37:07Z","timestamp":1759919827000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/97"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,27]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["vetsci12020097"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12020097","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,27]]}}}