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A total of 91 isolated yeasts were collected using the standard methods of microbial culture on agar medium followed by streaking on a plate at least three times until pure yeast colonies were formed. The API 20C AUX Kit and sequencing of the D1\/D2 domain of the 26S rRNA gene were used to identify the genera Candida spp., namely, C. glabrata (99% identification), C. tropicallis (99%), C. rugosa (98%), and Issatchenkia orientalis (99%). A total of 12 yeast strains (Dc4, 14, 18; Be1, 2, 7; Bu3, 4, 7; and Go10, 16, 19) were chosen for further analyses. The performance criteria included the ability to tolerate pH values between 3.5 and 7.5, total volatile fatty acids (TVFAs, 0, 0.25, 0.5, 1, 2, and 4% of broth medium), anaerobic growth rate, and in vitro gas production efficiency. First, when all strains were grown at pH values between 3.5 and 7.5, Bu3 and Dc18 performed better than the other strains. Second, at a ruminal pH of 6.5 and a TVFA concentration of between 2 and 4% of the broth medium, strain Bu3 was more resistant than the other strains. Under anaerobic conditions, all strains experienced a decline in viable cell counts when compared with those under aerobic conditions. However, compared to strains Dc14, Be1, Be2, Be7, and Bu3, strain Dc18 exhibited more viable cells under anaerobic conditions in broth medium. The response of strain Dc18 did not differ from those of strains Dc4, Bu4, Bu7, or G16. Strains Be7, Bu3, and Dc18 were used for an in vitro fermentation experiment involving incubation for 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h. Three ruminal cannulated dairy cows were used as donors of ruminal fluid. The treatments were run in triplicate. The addition of yeast culture had no effect on gas kinetics, gas accumulation, or the ratio of acetic acid and propionic acid, but led to significantly greater butyric acid concentrations at 24 h of incubation. In conclusion, strain Dc18 isolated from dairy cows is suitable for future studies of probiotic yeast development.<\/jats:p>","DOI":"10.3390\/vetsci12020155","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:34:32Z","timestamp":1739252072000},"page":"155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Isolation Ruminal Yeast from Ruminants on In Vitro Ruminal Fermentation"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1473-0206","authenticated-orcid":false,"given":"Krung","family":"Wilachai","sequence":"first","affiliation":[{"name":"School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4007-9000","authenticated-orcid":false,"given":"Pramote","family":"Paengkoum","sequence":"additional","affiliation":[{"name":"School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1251-168X","authenticated-orcid":false,"given":"Nittaya","family":"Taethaisong","sequence":"additional","affiliation":[{"name":"School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pirat","family":"Thitisak","sequence":"additional","affiliation":[{"name":"K.M.P.BIOTECH Co., Ltd. 188\/9 Moo 2, Klongtumru, Muangchonburi, Chonburi 20000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kriengsak","family":"Poonsuk","sequence":"additional","affiliation":[{"name":"K.M.P.BIOTECH Co., Ltd. 188\/9 Moo 2, Klongtumru, Muangchonburi, Chonburi 20000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J.","family":"Loor","sequence":"additional","affiliation":[{"name":"262 Animal Sciences Laboratory, Department of Animal Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois Urbana-Champaign, 1207 W Gregory Dr|M\/C 630, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8529-3452","authenticated-orcid":false,"given":"Siwaporn","family":"Paengkoum","sequence":"additional","affiliation":[{"name":"Program in Agriculture, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University, Nakhon Ratchasim 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.anifeedsci.2006.09.011","article-title":"Daily rumen pH pattern of loose-housed dairy cattle as affected by feeding pattern and live yeast supplementation","volume":"136","author":"Bach","year":"2007","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1177\/1756283X11428502","article-title":"Efficacy and safety of the probiotic Saccharomyces boulardii for the prevention and therapy of gastrointestinal disorders","volume":"5","author":"Kelesidis","year":"2012","journal-title":"Ther. Adv. Gastroenterol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.3168\/jds.S0022-0302(88)79894-X","article-title":"Influence of Addition of Yeast Culture Supplement to Diets of Lactating Cows on Ruminal Fermentation and Microbial Populations","volume":"71","author":"Harrison","year":"1988","journal-title":"J. Dairy Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1046\/j.1472-765x.2000.00826.x","article-title":"Selection of Saccharomyces cerevisiae strains for use as a microbial feed additive","volume":"31","author":"Agarwal","year":"2000","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.3168\/jds.2010-3277","article-title":"Differing effects of 2 active dried yeast (Saccharomyces cerevisiae) strains on ruminal acidosis and methane production in nonlactating dairy cows","volume":"94","author":"Chung","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1099\/00221287-81-2-453","article-title":"Yeasts and moulds in the bovine rumen","volume":"81","author":"Lund","year":"1974","journal-title":"Microbiology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1099\/00221287-121-1-273","article-title":"Yeasts in the rumen contents of musk oxen","volume":"121","author":"Lund","year":"1980","journal-title":"Microbiology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1080\/1745039X.2016.1163002","article-title":"Effects of dietary addition of cellulase and a Saccharomyces cerevisiae fermentation product on nutrient digestibility, rumen fermentation and enteric methane emissions in growing goats","volume":"70","author":"Lu","year":"2016","journal-title":"Arch. Anim. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"169","DOI":"10.2323\/jgam.56.169","article-title":"Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri Island in Japan","volume":"56","author":"Nakase","year":"2010","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/lam.12078","article-title":"Isolation, identification and growth determination of lactic acid-utilizing yeasts from the ruminal fluid of dairy cattle","volume":"57","author":"Sirisan","year":"2013","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1590\/S1516-89132009000400001","article-title":"A New Biosurfactant Produced by Candida glabrata UCP 1002: Characteristics of Stability and Application in Oil Recovery","volume":"52","author":"Luna","year":"2009","journal-title":"Braz. Arch. Biol. Technol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/BF00436458","article-title":"Isolation of yeasts from bovine milk in Belgium","volume":"135","author":"Lagneau","year":"1996","journal-title":"Mycopathologia"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"115565","DOI":"10.1016\/j.anifeedsci.2022.115565","article-title":"Effects of supplementing a yeast culture in a pelleted total mixed ration on fiber degradation, fermentation parameters, and the bacterial community in the rumen of sheep","volume":"296","author":"Wang","year":"2023","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Suntara, C., Cherdthong, A., Wanapat, M., Uriyapongson, S., Leelavatcharamas, V., Sawaengkaew, J., Chanjula, P., and Foiklang, S. (2021). Isolation and Characterization of Yeasts from Rumen Fluids for Potential Use as Additives in Ruminant Feeding. Vet. Sci., 8.","DOI":"10.3390\/vetsci8030052"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"115777","DOI":"10.1016\/j.anifeedsci.2023.115777","article-title":"Evaluation of legumes for fermentability and protein fractions using in vitro rumen fermentation","volume":"305","author":"Tunkala","year":"2023","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_16","first-page":"143","article-title":"The Role of Probiotics in Promoting Dairy Production","volume":"21","author":"Chiquette","year":"2009","journal-title":"WCDS Adv. Dairy Technol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ding, G., Chang, Y., Zhao, L., Zhou, Z., Ren, L., and Meng, Q. (2014). Effect of Saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios. J. Anim. Sci. Biotechnol., 5.","DOI":"10.1186\/2049-1891-5-24"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/lam.12475","article-title":"Selection and identification of oleaginous yeast isolated from soil, animal feed and ruminal fluid for use as feed supplement in dairy cattle","volume":"61","author":"Paserakung","year":"2015","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S2095-3119(14)60830-3","article-title":"Feeding of yeast (Candida spp.) improves in vitro ruminal fermentation of fibrous substrates","volume":"14","author":"Marrero","year":"2015","journal-title":"J. Integr. Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"211","DOI":"10.5713\/ajas.15.0166","article-title":"Effects of Candida norvegensis live cells on in vitro oat straw rumen fermentation","volume":"29","author":"Ruiz","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.anifeedsci.2007.04.019","article-title":"Effects of active dry yeasts on the rumen microbial ecosystem: Past, present and future","volume":"145","author":"Walker","year":"2008","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.2527\/1995.7361811x","article-title":"Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep","volume":"73","author":"Newbold","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Vorlaphim, T., Paengkoum, P., Purba, R.A.P., Yuangklang, C., Paengkoum, S., and Schonewille, J.T. (2021). Treatment of Rice Stubble with Pleurotus ostreatus and Urea Improves the Growth Performance in Slow-Growing Goats. Animals, 11.","DOI":"10.20944\/preprints202103.0335.v1"},{"key":"ref_24","unstructured":"Cuppuccino, J.G. (2012). Microbiology a Laboratory Manual, Benjamin Cummings. [7th ed.]."},{"key":"ref_25","first-page":"71","article-title":"Yeast of the World: Morpholoth, Physiology, Sequences and Identification","volume":"7","author":"Boekhout","year":"2002","journal-title":"Mycologist"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jgeb.2014.01.001","article-title":"Phylogenetic analysis of isolated biofuel yeasts based on 5.8S-ITS rDNA and D1\/D2 26S rDNA sequences","volume":"12","author":"Hesham","year":"2014","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_27","first-page":"7","article-title":"Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid","volume":"28","author":"Menke","year":"1988","journal-title":"Anim. Res."},{"key":"ref_28","first-page":"231","article-title":"Piper oil decreases In vitro methane production with shifting ruminal fermentation in a variety of diets","volume":"25","author":"Purba","year":"2021","journal-title":"Int. J. Agric. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1007\/s11250-019-01799-0","article-title":"Effects of crude protein and undegradable intake protein on growth performance, nutrient utilization, and rumen fermentation in growing Thai-indigenous beef cattle","volume":"51","author":"Paengkoum","year":"2019","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_30","first-page":"499","article-title":"The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage","volume":"92","author":"McDonald","year":"1979","journal-title":"Agric. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.3168\/jds.S0022-0302(61)89956-6","article-title":"Volatile fatty acid analyses of blood and rumen fluid by gas chromatography","volume":"44","author":"Erwin","year":"1961","journal-title":"J. Dairy Sci."},{"key":"ref_32","unstructured":"Steel, R.G.D., and Torrie, J.H. (1980). Principles and Procedures of Statistics, a Biometrical Approach, McGraw-Hill Kogakusha, Ltd."},{"key":"ref_33","unstructured":"Schaechter, M. (2009). Rumen. Encyclopedia of Microbiology, Academic Press. [3rd ed.]."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gao, K., and Geng, C. (2022). Alterations in the rumen bacterial communities and metabolites of finishing bulls fed high-concentrate diets supplemented with active dry yeast and yeast culture. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.908244"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e13579","DOI":"10.1111\/asj.13579","article-title":"Potential protective effects of thiamine supplementation on the ruminal epithelium damage during subacute ruminal acidosis","volume":"92","author":"Ma","year":"2021","journal-title":"Anim. Sci. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"R1515","DOI":"10.1152\/ajpregu.00120.2010","article-title":"Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis","volume":"300","author":"Steele","year":"2011","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1365-2672.1977.tb00745.x","article-title":"Mechanism of Resistance of Saccharomyces bailii to Benzoic, Sorbic and Other Weak Acids Used as Food Preservatives","volume":"43","author":"Warth","year":"1977","journal-title":"J. Appl. Bateriol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1079\/BJN19960029","article-title":"Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants","volume":"76","author":"Newbold","year":"1996","journal-title":"Br. J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.3168\/jds.2008-1414","article-title":"Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants","volume":"92","author":"Desnoyers","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/j.1439-0396.1997.tb00734.x","article-title":"In vitro gas production: A technique revisited","volume":"77","author":"Makkar","year":"1997","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.5713\/ajas.2000.1084","article-title":"Fermentation Characteristics and Microbial Protein Synthesis in an In Vitro System Using Cassava, Rice Straw and Dried Ruzi Grass as Substrates","volume":"13","author":"Sommart","year":"2000","journal-title":"Asian-Aus. J. Anim. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4252","DOI":"10.3168\/jds.2019-16904","article-title":"Liquid hot water treatment of rice straw enhances anaerobic degradation and inhibits methane production during in vitro ruminal fermentation","volume":"103","author":"Zhang","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1017\/ASC200693","article-title":"Dose effect of live yeasts on rumen microbial communities and fermentations during butyric latent acidosis in sheep: New type of interaction","volume":"82","author":"Brossard","year":"2006","journal-title":"Anim. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"36","DOI":"10.5713\/ajas.2013.13298","article-title":"Influence of Yeast Fermented Cassava Chip Protein (YEFECAP) and Roughage to Concentrate Ratio on Ruminal Fermentation and Microorganisms Using In vitro Gas Production Technique","volume":"27","author":"Polyorach","year":"2014","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Pacheco, R.D.L., Souza, J.M., Marino, C.T., Bastos, J., Martins, C.L., Rodrigues, P.H.M., Arrigoni, M.D.B., and Millen, D.D. (2023). Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1090107"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lu, G., Li, Y., Mao, K., Zang, Y., Zhao, X., Qiu, Q., Qu, M., and Ouyang, K. (2022). Effects of Rumen-Protected Creatine Pyruvate on Meat Quality, Hepatic Gluconeogenesis, and Muscle Energy Metabolism of Long-Distance Transported Beef Cattle. Front. Anim. Sci, 3.","DOI":"10.3389\/fanim.2022.904503"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5401","DOI":"10.3168\/jds.2015-10563","article-title":"Effects of Saccharomyces cerevisiae fermentation products on dairy calves: Ruminal fermentation, gastrointestinal morphology, and microbial community","volume":"99","author":"Xiao","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e13791","DOI":"10.1111\/asj.13791","article-title":"Sodium butyrate administration modulates the ruminal villus height, inflammation-related gene expression, and plasma hormones concentration in dry cows fed a high-fiber diet","volume":"93","author":"Fukumori","year":"2022","journal-title":"Anim. Sci. J."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Pinloche, E., McEwan, N., Marden, J.-P., Bayourthe, C., Auclair, E., and Newbold, C.J. (2013). The Effects of a Probiotic Yeast on the Bacterial Diversity and Population Structure in the Rumen of Cattle. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067824"},{"key":"ref_50","first-page":"169","article-title":"Yeasts on in vitro digestibility of diets with different levels of roughage","volume":"16","author":"Igueiroa","year":"2015","journal-title":"Ci\u00eanc. Anim. Bras."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.3168\/jds.S0022-0302(02)74345-2","article-title":"Effects of Saccharomyces cerevisiae Culture and Saccharomyces cerevisiae Live Cells on In Vitro Mixed Ruminal Microorganism Fermentation","volume":"85","author":"Lynch","year":"2002","journal-title":"J. Dairy Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/155\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:31:10Z","timestamp":1760027470000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":51,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["vetsci12020155"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12020155","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]}}}