{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:54:13Z","timestamp":1760057653193,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32072933","32072923"],"award-info":[{"award-number":["32072933","32072923"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Off-season breeding is a method of encouraging animals through manual intervention to reproduce during the non-breeding season. The egg-laying period of the Zhedong white goose usually starts from September and ends in April of the following year. During the summer months from May to August, with long daylight hours and high temperatures, the white geese rest and do not lay eggs. This study explored the effects of off-season breeding on egg production performance, serum biochemical indicators, and hormone secretion in Zhedong white goose. The experiment was divided into normal breeding and off-seasonal breeding. The results suggested that the peak laying period lasted 3 months, and egg production was 15.62% higher than that in the control group in the off-season breeding group. The off-season breeding group had a 4.13% higher egg fertilization rate in November (laying period) than the control group, and the hatching rate of hatched eggs and fertilized eggs in the peak laying period was 2.77% and 2.3% higher, respectively, than the control group. The experimental group showed significantly higher serum P, ALB (albumin), TG (triglyceride), and TC (total cholesterol) levels; serum AST (glutamic oxalacetic transaminase) activity; and CREA (creatinine) and BUN (blood urea nitrogen) levels than the control group at different time points. The serum LH (luteinizing hormone), FSH (follicle-stimulating hormone), P4 (progesterone), and E2 (estradiol) levels were significantly higher in the off-season breeding group than in the control group. The serum T3 (triiodothyronine) and T4 (Tetraiodothyronine) levels were significantly higher in the off-season breeding group than in the control group at different points in the breeding cycle. The control group showed a significantly higher gene expression of GnRH (gonadotropin releasing hormone) in the hypothalamus; GnRH, FSH, and LH in the pituitary; and GnRH in the ovary and significantly lower gene expression of VIP (vasoactine intestinal peptide) and PRL (prolactin) in the pituitary than the off-season breeding group. Thus, the off-season breeding of Zhedong white geese may prolong the peak laying period and improve egg production performance, thus enhancing the economic benefits of goose breeding.<\/jats:p>","DOI":"10.3390\/vetsci12020179","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T07:48:22Z","timestamp":1739778502000},"page":"179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigation of Off-Season Breeding Effects on Egg-Laying Performance, Serum Biochemical Parameters, and Reproductive Hormones in Zhedong White"],"prefix":"10.3390","volume":"12","author":[{"given":"Jiaqiao","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonggang","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waseem","family":"Ali","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-8363","authenticated-orcid":false,"given":"Zongping","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3748","DOI":"10.1002\/jsfa.12244","article-title":"Slaughter performance of the main goose breeds raised commercially in China and nutritional value of the meats of the goose breeds: A systematic review","volume":"103","author":"Yan","year":"2023","journal-title":"J. Sci. Food Agric."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1111\/jpn.13494","article-title":"Characterization of ovarian morphology and reproductive hormones in Zhedong white geese (Anser cygnoides domesticus) during the reproductive cycle","volume":"105","author":"Wang","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Liu, G., Guo, Z., Zhao, X., Sun, J., Yue, S., Li, M., Chen, Z., Ma, Z., and Zhao, H. (2023). Whole Genome Resequencing Identifies Single-Nucleotide Polymorphism Markers of Growth and Reproduction Traits in Zhedong and Zi Crossbred Geese. Genes, 14.","DOI":"10.3390\/genes14020487"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jgg.2012.03.002","article-title":"Identification of sheep ovary genes potentially associated with off-season reproduction","volume":"39","author":"Chen","year":"2012","journal-title":"J. Genet. Genom. = Yi Chuan Xue Bao"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"102193","DOI":"10.1016\/j.psj.2022.102193","article-title":"Identification of the differentially expressed genes in the leg muscles of Zhedong white geese (Anser cygnoides) reared under different photoperiods","volume":"101","author":"Hu","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.anireprosci.2017.03.009","article-title":"Induction of out-of-season egg laying by artificial photoperiod in Yangzhou geese and the associated endocrine and molecular regulation mechanisms","volume":"180","author":"Zhu","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/0300-9629(94)90143-0","article-title":"The effect of heparin injection on laying turkey hen very-low density lipoprotein metabolism in sexually immature and laying turkey hens","volume":"109","author":"Bacon","year":"1994","journal-title":"Comp. Biochem. Physiol. Part A Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104370","DOI":"10.1016\/j.psj.2024.104370","article-title":"Comparative analysis of reproductive hormones, serum biochemical indexes and ovarian metabolites in Muscovy breeder duck at different laying stages","volume":"103","author":"Yang","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102217","DOI":"10.1016\/j.psj.2022.102217","article-title":"Transcriptomic analysis of the liver in aged laying hens with different eggshell strength","volume":"102","author":"Han","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ygcen.2015.09.023","article-title":"An updated model to describe the neuroendocrine control of reproduction in chickens","volume":"227","author":"Baxter","year":"2016","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00441-010-1073-6","article-title":"Deep brain photoreceptors and a seasonal signal transduction cascade in birds","volume":"342","author":"Nakane","year":"2010","journal-title":"Cell Tissue Res"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.jvs.2019.04.488","article-title":"Review of serum biomarkers in carotid atherosclerosis","volume":"71","author":"Martinez","year":"2020","journal-title":"J. Vasc. Surg"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1080\/00071668.2023.2272982","article-title":"Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period","volume":"65","author":"Qi","year":"2024","journal-title":"Br. Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.meatsci.2017.01.012","article-title":"Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat","volume":"128","author":"Qu","year":"2017","journal-title":"Meat Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"106278","DOI":"10.1016\/j.anireprosci.2020.106278","article-title":"Meta-analysis: Supplementary artificial light and goose reproduction","volume":"214","author":"Liu","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.anireprosci.2008.05.073","article-title":"Supplementary artificial light to increase egg production of geese under natural lighting conditions","volume":"113","author":"Wang","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jtherbio.2019.07.004","article-title":"Role of gamma-aminobutyric acid in regulating feed intake in commercial broilers reared under normal and heat stress conditions","volume":"84","author":"Abdo","year":"2019","journal-title":"J. Therm. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"103201","DOI":"10.1016\/j.psj.2023.103201","article-title":"Dietary Chinese herbal mixture supplementation improves production performance by regulating reproductive hormones, antioxidant capacity, immunity, and intestinal health of broiler breeders","volume":"103","author":"Liu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1017\/S0029665108007015","article-title":"Nutritional factors affecting poultry bone health","volume":"67","author":"Fleming","year":"2008","journal-title":"Proc. Nutr. Soc."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sinclair-Black, M., Garcia, R.A., and Ellestad, L.E. (2023). Physiological regulation of calcium and phosphorus utilization in laying hens. Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1112499"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s11250-017-1434-1","article-title":"Non-phytate phosphorus requirement for broilers from 8 to 21 days of age under heat stress conditions","volume":"50","author":"Cardoso","year":"2018","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101244","DOI":"10.1016\/j.psj.2021.101244","article-title":"Effect of dietary calcium and phosphorus levels on growth, carcass characteristics and liver and kidney functions of growing Egyptian geese","volume":"100","author":"Alagawany","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.1002\/jsfa.6247","article-title":"Egg yolk: Structures, functionalities and processes","volume":"93","author":"Anton","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/s12291-022-01064-6","article-title":"Dependence of Seasonal Dynamics in Healthy People\u2019s Circulating Lipids and Carbohydrates on Regional Climate: Meta-Analysis","volume":"37","author":"Kuzmenko","year":"2022","journal-title":"Indian J. Clin. Biochem. IJCB"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nematbakhsh, S., Pei Pei, C., Selamat, J., Nordin, N., Idris, L.H., and Abdull Razis, A.F. (2021). Molecular Regulation of Lipogenesis, Adipogenesis and Fat Deposition in Chicken. Genes, 12.","DOI":"10.3390\/genes12030414"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0305-0491(00)00171-1","article-title":"Role of hepatic lipogenesis in the susceptibility to fatty liver in the goose (Anser anser)","volume":"126","author":"Mourot","year":"2000","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/0016-6480(81)90299-9","article-title":"The effect of short-term starvation on pituitary and plasma LH, plasma estradiol and progesterone, and on pituitary response to LH-RH in the laying hen (Gallus domesticus)","volume":"43","author":"Tanabe","year":"1981","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_28","first-page":"85","article-title":"Serum aminotransferases and bilirubin levels in malaria patients","volume":"1","author":"Enemor","year":"2005","journal-title":"Int. J. Nat. Appl. Sci."},{"key":"ref_29","first-page":"178","article-title":"Comparative Analysis of Nutrient Digestibility and Serum Biochemical Indexes among Different Goose Breeds","volume":"35","author":"Tao","year":"2015","journal-title":"Life Sci"},{"key":"ref_30","first-page":"649","article-title":"Blood biochemical indexes of northeast white goose and seed goose","volume":"79","author":"Kang","year":"2006","journal-title":"Chin. Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/00071668.2022.2152651","article-title":"Comparative transcriptomic analysis reveals candidate genes for seasonal breeding in the male Lion-Head goose","volume":"64","author":"Lin","year":"2023","journal-title":"Br. Poult. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s11250-023-03855-2","article-title":"Interactive effects of dietary protein and nano-chitosan on growth performance, immune response, and histological aspects of lymphoid organs in broiler chickens","volume":"56","author":"Mansour","year":"2024","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/0378-4320(96)01556-4","article-title":"Strategies in avian breeding cycles","volume":"42","author":"Sharp","year":"1996","journal-title":"Anim. Reprod. Sci."},{"key":"ref_34","first-page":"328","article-title":"Red jungle fowl (Gallus gallus) as a model for studying the molecular mechanism of seasonal reproduction","volume":"80","author":"Ono","year":"2009","journal-title":"Anim. Sci. J. = Nihon Chikusan Gakkaiho"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1159\/000127079","article-title":"Regulation of chicken gonadotropin-releasing hormone-I mRNA in incubating, nest-deprived and laying bantam hens","volume":"63","author":"Dunn","year":"1996","journal-title":"Neuroendocrinology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ygcen.2009.03.010","article-title":"Gonadotropin-inhibitory hormone receptor signaling and its impact on reproduction in chickens","volume":"163","author":"McFarlane","year":"2009","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"486","DOI":"10.3382\/ps\/pew340","article-title":"Seasonal and photoperiodic regulation of reproductive hormones and related genes in Yangzhou geese","volume":"96","author":"Yang","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Gumu\u0142ka, M., Avital-Cohen, N., and Rozenboim, I. (2021). Determination of Annual Plasma Hormone Levels Associated with Reproduction in Long-Day Breeding Domestic Geese. Animals, 11.","DOI":"10.3390\/ani11082363"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"104764","DOI":"10.1016\/j.psj.2025.104764","article-title":"The hematopoietic function, histological characteristics, and transcriptome profiling of Wanxi white geese ovary during nesting and late-laying stages","volume":"104","author":"Zhao","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_40","first-page":"355","article-title":"Effect of severe dietary protein, nonprotein calories or feed restriction during gestation on postnatal growth of progeny in swine","volume":"51","author":"Pond","year":"1987","journal-title":"Growth"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"102674","DOI":"10.1016\/j.psj.2023.102674","article-title":"Comparison of the effect of quercetin and daidzein on production performance, anti-oxidation, hormones, and cecal microflora in laying hens during the late laying period","volume":"102","author":"Liu","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1677\/joe.0.1680001","article-title":"Growth hormone: Roles in female reproduction","volume":"168","author":"Hull","year":"2001","journal-title":"J. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1530\/ror.0.0050175","article-title":"Growth hormone: A reproductive endocrine-paracrine regulator?","volume":"5","author":"Hull","year":"2000","journal-title":"Rev. Reprod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.3382\/ps.0681126","article-title":"Effects of thyroidectomy on egg production, molt, and plasma thyroid hormone concentrations of turkey hens","volume":"68","author":"Lien","year":"1989","journal-title":"Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"105160","DOI":"10.1016\/j.yhbeh.2022.105160","article-title":"Sex differences in seasonal brain plasticity and the neuroendocrine regulation of vocal behavior in songbirds","volume":"142","author":"Rose","year":"2022","journal-title":"Horm. Behav."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1016\/j.psj.2019.12.015","article-title":"The effect of parachlorophenylalanine treatment on the activity of gonadal and lactotrophic axes in native Polish crested chickens stimulated to broodiness","volume":"99","author":"Hrabia","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"161","DOI":"10.3382\/ps.2011-01439","article-title":"The effect of active immunization against vasoactive intestinal peptide (VIP) and inhibin on reproductive performance of aging White Leghorn roosters","volume":"91","author":"Heiblum","year":"2012","journal-title":"Poult. Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/179\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:36:22Z","timestamp":1760027782000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/2\/179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,17]]},"references-count":47,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["vetsci12020179"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12020179","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2025,2,17]]}}}