{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:55:48Z","timestamp":1760057748888,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["2009\/06702-1","CEPID-CTC 2013\/08135-2","2021\/05445-7"],"award-info":[{"award-number":["2009\/06702-1","CEPID-CTC 2013\/08135-2","2021\/05445-7"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2009\/06702-1","CEPID-CTC 2013\/08135-2","2021\/05445-7"],"award-info":[{"award-number":["2009\/06702-1","CEPID-CTC 2013\/08135-2","2021\/05445-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Major histocompatibility complex class I (MHC-I) gene expression in the placenta is modulated to tailor the maternal immune response to fetal antigens during pregnancy. This study evaluated MHC-I expression through immunohistochemistry (IHC) using an anti-mouse preimplantation embryo development (PED) clone Qa-2 and anti-bovine leukocyte antigen I (BoLA) monoclonal antibody clone IL-A88 (n = 23), as well as RT-qPCR (n = 17) for classical and non-classical (BoLA-NC) genes in control and cloned bovine placentomes during early and near-term gestation. Control samples showed minimal Qa-2 protein expression in early gestation, with intense labeling in trophoblasts and the maternal uterine epithelium near term. In contrast, cloned samples exhibited intense Qa-2 labeling in both maternal and trophoblastic epithelia at both stages, while trophoblast giant cells (TGCs), located apposed to the maternal epithelium, showed no labeling. Control samples exhibited intense IL-A88 labeling in the maternal epithelium at both stages. In cloned samples, weak to no labeling was observed in early gestation, with intense labeling in maternal and fetal epithelium near term. RT-qPCR revealed significant upregulation of BoLA-NC3 in early gestation, with sustained elevated expression in cloned samples in the near term. These findings suggest that altered BoLA protein expression and gene regulation in cloned pregnancies may contribute to pregnancy complications and increased losses.<\/jats:p>","DOI":"10.3390\/vetsci12030196","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T06:39:07Z","timestamp":1740119947000},"page":"196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of MHC I Isoforms in Bovine Placentomes: Impact of Cloning"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5240-6959","authenticated-orcid":false,"given":"Rodrigo da Silva Nunes","family":"Barreto","sequence":"first","affiliation":[{"name":"RB Lab, College of Agricultural and Veterinary Sciences, Sao Paulo State University, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3382-579X","authenticated-orcid":false,"given":"Ana Carolina Furlanetto","family":"Man\u00e7anares","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chill\u00e1n 3780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4979-115X","authenticated-orcid":false,"given":"Maria Angelica","family":"Miglino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Mar\u00edlia (UNIMAR), Mar\u00edlia 17525-902, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0372-4920","authenticated-orcid":false,"given":"Fl\u00e1vio Vieira","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of S\u00e3o Paulo, Pirassununga 13635-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian de Jesus","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1053\/plac.2000.0505","article-title":"Current Topic: The Role of Growth Hormone and Insulin-like Growth Factors for Placental Growth and Development","volume":"21","author":"Zumkeller","year":"2000","journal-title":"Placenta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.humimm.2006.10.012","article-title":"HLA-G Expression Is a Fundamental Prerequisite to Pregnancy","volume":"68","author":"Rizzo","year":"2007","journal-title":"Hum. 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