{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T12:46:49Z","timestamp":1770468409350,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key R&amp;D Project of Sichuan Provincial Science and Technology Department","award":["2024YFFK0146"],"award-info":[{"award-number":["2024YFFK0146"]}]},{"name":"Key R&amp;D Project of Sichuan Provincial Science and Technology Department","award":["32473109"],"award-info":[{"award-number":["32473109"]}]},{"name":"Key R&amp;D Project of Sichuan Provincial Science and Technology Department","award":["NSF2022ZR03"],"award-info":[{"award-number":["NSF2022ZR03"]}]},{"name":"Key R&amp;D Project of Sichuan Provincial Science and Technology Department","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]},{"name":"National Natural Science Foundation of China","award":["2024YFFK0146"],"award-info":[{"award-number":["2024YFFK0146"]}]},{"name":"National Natural Science Foundation of China","award":["32473109"],"award-info":[{"award-number":["32473109"]}]},{"name":"National Natural Science Foundation of China","award":["NSF2022ZR03"],"award-info":[{"award-number":["NSF2022ZR03"]}]},{"name":"National Natural Science Foundation of China","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["2024YFFK0146"],"award-info":[{"award-number":["2024YFFK0146"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["32473109"],"award-info":[{"award-number":["32473109"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["NSF2022ZR03"],"award-info":[{"award-number":["NSF2022ZR03"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study investigated the protective effects and mechanisms of cow placenta peptides (CPP) on intestinal barrier damage in aging model mice. Forty-eight male ICR mice were assigned to four groups: a control group (N), an aging model group (M), a CPP treatment group (T), and a vitamin C treatment group (P). Groups T and P received oral administration of CPP (2000 mg\/kg\/day) and vitamin C (100 mg\/kg\/day), respectively, while groups M, T, and P were subjected to intraperitoneal injections of D-galactose (D-gal) (300 mg\/kg\/day). Group N received an equivalent volume of normal saline via intraperitoneal injection. Treatments were administered once daily for 8 weeks. The results demonstrated that CPP significantly alleviated D-galactose-induced intestinal structural damage, increasing the villus height-to-crypt depth ratio and reducing serum diamine oxidase (DAO) and lipopolysaccharide (LPS) levels. CPP notably alleviated intestinal oxidative stress and inflammation, restored tight junction expression, and enhanced intestinal barrier integrity. Transcriptome sequencing identified 1396 DEGs associated with CPP\u2019s effects, highlighting TLR4, IL-1\u03b2, and Mmp9 as core regulatory genes through protein\u2013protein interaction network analysis. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses implicated the TLR4\/NF-\u03baB signaling pathway, which was further validated. Western blotting confirmed that CPP significantly down-regulated TLR4, IKK\u03b2, and p-NF-\u03baB p65 protein expression in the intestines of aging mice. In conclusion, CPP effectively alleviates D-gal-induced intestinal barrier damage in aging mice by enhancing antioxidant defense and inhibiting the TLR4\/NF-\u03baB signaling pathway, thereby diminishing inflammation and protecting intestinal barrier integrity.<\/jats:p>","DOI":"10.3390\/vetsci12030229","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T05:52:16Z","timestamp":1740981136000},"page":"229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cow Placenta Peptides Ameliorate D-Galactose-Induced Intestinal Barrier Damage by Regulating TLR\/NF-\u03baB Pathway"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9653-3973","authenticated-orcid":false,"given":"Yuquan","family":"Zhao","sequence":"first","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijian","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeru","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Luo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunshan","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyan","family":"Ding","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuxing","family":"Hao","sequence":"additional","affiliation":[{"name":"Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1816-9328","authenticated-orcid":false,"given":"Yixin","family":"Huang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5806","authenticated-orcid":false,"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, 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