{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T21:09:16Z","timestamp":1769720956302,"version":"3.49.0"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172803"],"award-info":[{"award-number":["32172803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20240101255JC"],"award-info":[{"award-number":["20240101255JC"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Jilin Province, China","award":["32172803"],"award-info":[{"award-number":["32172803"]}]},{"name":"Natural Science Foundation of Jilin Province, China","award":["20240101255JC"],"award-info":[{"award-number":["20240101255JC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The cryopreservation of testicular tissues meets the demands for the germplasm preservation of humans and animals. Previously, we reported on the cryopreservation of bovine testicular tissues. To further evaluate the viability of these tissues, subcutaneous xenotransplantation of the frozen\u2013thawed calf testicular tissues was performed with castrated nude mice as the recipients. After 28 days (D28), the survival and development of the grafts were examined. The grafts from 1-day-old (D1) calf testes were recovered and angiogenesis around the grafts was observed. Histologically, the seminiferous cords in the grafts were well maintained and capillaries in the interstitium were observed. Quantitative real-time PCR (qRT-PCR) analysis showed that the grafts expressed germline genes Gfr\u03b1-1, C-kit, and Sycp3 and somatic genes Sox9, Acta2, and Star. The expressions of C-kit, Sox9, Acta2, and Star were higher in 28D grafts than those in 1D and 30-day-old (30D) calf testicular controls. Together, we initially demonstrate that cryopreserved calf testicular tissues retain their viability and developmental capacity after xenotransplantation.<\/jats:p>","DOI":"10.3390\/vetsci12030247","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T13:31:31Z","timestamp":1741095091000},"page":"247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Xenotransplantation of Cryopreserved Calf Testicular Tissues"],"prefix":"10.3390","volume":"12","author":[{"given":"Yansen","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqian","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0670-2452","authenticated-orcid":false,"given":"Xueming","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/nature00918","article-title":"Sperm from neonatal mammalian testes grafted in mice","volume":"418","author":"Honaramooz","year":"2002","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"F1","DOI":"10.1530\/REP-18-0533","article-title":"Fertility Preservation: Testicular transplantation for fertility preservation: Clinical potential and current challenges","volume":"158","author":"Kilcoyne","year":"2019","journal-title":"Reproduction"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.fertnstert.2020.01.038","article-title":"Progress in translational reproductive science: Testicular tissue transplantation and in vitro spermatogenesis","volume":"113","author":"Pelzman","year":"2020","journal-title":"Fertil. 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