{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:05:38Z","timestamp":1782936338179,"version":"3.54.5"},"reference-count":63,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Doctoral Research Start-up Fund of Zhoukou Normal University","award":["70294"],"award-info":[{"award-number":["70294"]}]},{"name":"Doctoral Research Start-up Fund of Zhoukou Normal University","award":["32102708"],"award-info":[{"award-number":["32102708"]}]},{"name":"Doctoral Research Start-up Fund of Zhoukou Normal University","award":["232102311060"],"award-info":[{"award-number":["232102311060"]}]},{"name":"National Natural Science Foundation of China","award":["70294"],"award-info":[{"award-number":["70294"]}]},{"name":"National Natural Science Foundation of China","award":["32102708"],"award-info":[{"award-number":["32102708"]}]},{"name":"National Natural Science Foundation of China","award":["232102311060"],"award-info":[{"award-number":["232102311060"]}]},{"name":"Henan Provincial Science and Technology Research Project","award":["70294"],"award-info":[{"award-number":["70294"]}]},{"name":"Henan Provincial Science and Technology Research Project","award":["32102708"],"award-info":[{"award-number":["32102708"]}]},{"name":"Henan Provincial Science and Technology Research Project","award":["232102311060"],"award-info":[{"award-number":["232102311060"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Anaplasma phagocytophilum is a zoonotic pathogen transmitted by arthropod vectors. The pathogen infects various vertebrate hosts, causing mild to severe illness. Molecular studies have demonstrated that A. phagocytophilum exhibits a high level of genetic diversity, with two A. phagocytophilum-related variants identified in several countries. This study represents the first application of PCR amplification and restriction fragment length polymorphism (PCR-RFLP) in conjunction with DNA sequencing to investigate the frequency and phylogenetic relationships of A. phagocytophilum and its related strains in cattle from China. A total of 662 bovine blood samples were collected from diverse regions within Henan Province, China, and pathogen DNA was detected in 75 samples, comprising 11.33% of the total. PCR-RFLP analysis identified three strains with frequency rates of 2.87% (19\/662) for A. phagocytophilum, 11.33% (75\/662) for A. phagocytophilum-like 1, and 3.22% (22\/662) for A. phagocytophilum-like 2. Additionally, co-infections involving A. phagocytophilum and A. phagocytophilum-like 1 were observed as well as between A. phagocytophilum-like 1 and A. phagocytophilum-like 2. Anaplasma phagocytophilum-like strains 1 and 2 should be considered when diagnosing bovine anaplasmosis. Despite recent molecular studies of A. phagocytophilum-related strains, there remains a shortage of data concerning vector capability, the epidemiology of the disease, clinical signs, and genetic diversity of the pathogens. Thus, large-scale investigations involving animals and tick vectors are necessary to obtain more detailed information concerning the etiology of anaplasmosis.<\/jats:p>","DOI":"10.3390\/vetsci12030252","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T06:02:13Z","timestamp":1741240933000},"page":"252","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum and Related Strains in Cattle from Henan, China"],"prefix":"10.3390","volume":"12","author":[{"given":"Yaqun","family":"Yan","sequence":"first","affiliation":[{"name":"College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466000, China"},{"name":"Field Observation and Research Station of Green Agriculture in Dancheng County, Dancheng 477150, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongli","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466000, China"},{"name":"Field Observation and Research Station of Green Agriculture in Dancheng County, Dancheng 477150, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466000, China"},{"name":"Field Observation and Research Station of Green Agriculture in Dancheng County, Dancheng 477150, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6866-1479","authenticated-orcid":false,"given":"Shuhua","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466000, China"},{"name":"Field Observation and Research Station of Green Agriculture in Dancheng County, Dancheng 477150, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changshen","family":"Ning","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","article-title":"Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and \u2018HGE agent\u2019 as subjective synonyms of Ehrlichia phagocytophila","volume":"51","author":"Dumler","year":"2001","journal-title":"Int. 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