{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:02:42Z","timestamp":1760058162406,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Li\u00e8ge, Belgium, \u201cCr\u00e9dits sectoriels de la Recherche en Sciences de la Sant\u00e9\u201d 2020\u20132022"},{"name":"\u201cBourse d\u2019Etudes internationales\u201d of \u201cWallonie-Bruxelles International\u201d (WBI)"},{"name":"University of Liege, Belgium in 2022\u20132023 (\u201cCr\u00e9dits sectoriels de la Recherche en Sciences de la Sant\u00e9\u201d 2021\u20132023"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Despite their prevalence in Europe, the source of contamination of humans by Attaching-Effacing Shigatoxigenic Escherichia coli (AE-STEC) O80:H2 remains unidentified. This study aimed to assess a procedure based on non-melibiose fermentation and resistance to tellurite to isolate AE-STEC and enteropathogenic (EPEC) O80:H2 from healthy cattle. The genome sequences of 40 calf and human AE-STEC and EPEC O80:H2 were analyzed: (i) none harbored the mel operon, but the 70mel DNA sequence instead; (ii) the ter-type 1 operon was detected in 16 EPEC and stx1a or stx2a AE-STEC, while no ter-type 1 operon was detected in the remaining 24 EPEC and stx2d AE-STEC. The 21 calf AE-STEC and EPEC O80:H2 were tested phenotypically: (i) none fermented melibiose on melibiose-MacConkey agar plates; (ii) ten of the 11 ter-type 1-positive strains had Minimal Inhibitory Concentrations (MIC) \u2265 128 \u00b5g\/mL to potassium tellurite; (iii) conversely, the ten ter-negative strains had MIC of two \u00b5g\/mL. Accordingly, enrichment broths containing two \u00b5g\/mL of potassium tellurite and inoculated with one high MIC (\u2265256 \u00b5g\/mL) stx1a AE-STEC O80:H2 tested positive with the O80 PCR after overnight growth, but not the enrichment broths inoculated with one low MIC (two \u00b5g\/mL) EPEC. Nevertheless, neither AE-STEC nor EPEC O80:H2 were recovered from 96 rectal fecal samples collected from healthy cattle at one slaughterhouse after overnight growth under the same conditions. In conclusion, this procedure may help to isolate stx1a and stx2a AE-STEC and EPEC O80:H2, but not stx2d AE-STEC that are tellurite sensitive, and new surveys using different procedures are necessary to identify their animal source, if any.<\/jats:p>","DOI":"10.3390\/vetsci12030274","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T07:02:16Z","timestamp":1741935736000},"page":"274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Non-Melibiose Fermentation and Tellurite Resistance by Shigatoxigenic and Enteropathogenic Escherichia coli O80:H2 from Diseased Calves: Comparison with Human Shigatoxigenic E. coli O80:H2"],"prefix":"10.3390","volume":"12","author":[{"given":"Rie","family":"Ikeda","sequence":"first","affiliation":[{"name":"Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8500-9359","authenticated-orcid":false,"given":"Keiji","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Korsak","sequence":"additional","affiliation":[{"name":"Food Inspection, Department of Food Sciences, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-No\u00ebl","family":"Duprez","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-7364","authenticated-orcid":false,"given":"Damien","family":"Thiry","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8520-0834","authenticated-orcid":false,"given":"Jacques G.","family":"Mainil","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"ref_1","unstructured":"Morabito, S. (2014). Diarrheagenic Escherichia coli infections in humans. Pathogenic Escherichia coli: Molecular and Cellular Microbiology, Caister Academic Press."},{"key":"ref_2","unstructured":"Morabito, S. (2014). Pathogenic Escherichia coli in domestic mammals and birds. Pathogenic Escherichia coli: Molecular and Cellular Microbiology, Caister Academic Press."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1297-9716-43-13","article-title":"O157:H7 and O104:H4 Vero\/Shiga toxin-producing Escherichia coli outbreaks: Respective role of cattle and humans","volume":"43","author":"Haesebrouck","year":"2012","journal-title":"Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1128\/JCM.41.11.4930-4940.2003","article-title":"Association of genomic O island 122 of Escherichia coli EDL 933 with Verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and\/or serious disease","volume":"41","author":"Karmali","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.vetmic.2016.02.017","article-title":"Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC","volume":"202","author":"Fakih","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_6","first-page":"EHEC-0008-2013","article-title":"Escherichia coli O104:H4 pathogenesis: An enteroaggregative E. coli \/Shiga toxin-producing E. coli explosive cocktail of high virulence","volume":"2","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1800068","DOI":"10.2807\/1560-7917.ES.2019.24.8.1800068","article-title":"Paediatric haemolytic uraemic syndrome related to Shiga toxin-producing Escherichia coli, an overview of 10 years of surveillance in France, 2007 to 2016","volume":"24","author":"Bruyand","year":"2019","journal-title":"Euro. Surveill."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.3201\/eid2209.160304","article-title":"Enterohemorrhagic Escherichia coli hybrid pathotype O80:H2 as a new therapeutic challenge","volume":"22","author":"Soysal","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.3201\/eid2412.180272","article-title":"Emerging multidrug-resistant hybrid pathotype Shiga toxin-producing Escherichia coli O80 and related strains of Clonal Complex 165, Europe","volume":"24","author":"Cointe","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","unstructured":"(2024, June 03). ANSES (Agence nationale de s\u00e9curit\u00e9 sanitaire de l\u2019alimentation, de l\u2019environnement et du travail), Avis relatif \u00e0 la d\u00e9finition des souches pathog\u00e8nes d\u2019Escherichia coli productrices de Shiga-toxines, Saisine n\u00b0 2020-SA-0095, n\u00b02016-SA-0121, 2010-SA-0031. Available online: https:\/\/www.anses.fr\/fr\/system\/files\/BIORISK2020SA0095.pdf."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"EFSA-ECDC (European Food Safety Authority\u2014European Centre for Disease Prevention and Control) (2023). The European Union one health 2022 zoonoses report. EFSA J., 21, e8442.","DOI":"10.2903\/j.efsa.2023.8442"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.3201\/eid2312.170450","article-title":"Enteropathogenic Escherichia coli O80:H2 in young calves with diarrhea, Belgium","volume":"23","author":"Thiry","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1111\/jam.14759","article-title":"Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2","volume":"130","author":"Habets","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Soleau, N., Ganet, S., Werlen, S., Collignon, L., Cointe, A., Bonacorsi, S., and Sergentet, D. (2024). First Isolation of the heteropathotype Shiga toxin-producing and extra-intestinal pathogenic (STEC-ExPEC) E. coli O80:H2 in French healthy cattle: Genomic characterization and phylogenetic position. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25105428"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Bizot, E., Cointe, A., Smadja, N., Sergentet, D., Lef\u00e8vre, S., Weill, F.-X., Levy, C., Cohen, R., Mariani-Kurkdjian, P., and Bonacorsi, S. (2022). Improved molecular diagnosis and culture of the emerging heteropathotype enterohemorrhagic Escherichia coli O80:H2 using its non-melibiose-fermenting and antibiotic-resistance properties. J. Clin. Microbiol., 60.","DOI":"10.1128\/JCM.01530-21"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.fm.2016.05.005","article-title":"Variable tellurite resistance profiles of clinically-relevant Shiga toxin-producing Escherichia coli (STEC) influence their recovery from foodstuffs","volume":"59","author":"Kerangart","year":"2016","journal-title":"Food Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1007\/s00284-018-1444-x","article-title":"Tellurite resistance in Shiga toxin-producing Escherichia coli","volume":"75","author":"Lewis","year":"2018","journal-title":"Curr. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.ijfoodmicro.2017.12.016","article-title":"Tellurite resistance profiles and performance of different chromogenic agars for detection of non-O157 Shiga toxin-producing Escherichia coli","volume":"266","author":"Fan","year":"2018","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/dnares\/8.1.11","article-title":"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12","volume":"8","author":"Hayashi","year":"2001","journal-title":"DNA Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"111.e5","DOI":"10.1016\/j.cmi.2018.07.023","article-title":"Characteristics of Shiga toxin-producing and enteropathogenic Escherichia coli of the emerging serotype O80:H2 isolated from humans and diarrheic calves in Belgium","volume":"25","author":"Thiry","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ikeda, R., Nakamura, K., Saulmont, M., Habets, A., Duprez, J.-N., Korsak, N., Hayashi, T., Thiry, D., and Mainil, J.G. (2023). Escherichia coli O80 in healthy cattle: Absence of Shigatoxigenic and enteropathogenic E. coli O80:H2 and (phylo)genomics of non-clonal complex 165 E. coli O80. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020230"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nguyen, T.T.H., Kikuchi, T., Tokunaga, T., SIyoda, S., and Iguchi, A. (2021). Diversity of the tellurite resistance gene operon in Escherichia coli. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.681175"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4690","DOI":"10.1128\/JB.184.17.4690-4698.2002","article-title":"Genomic variability of O islands encoding tellurite resistance in enterohemorrhagic Escherichia coli O157:H7 isolates","volume":"184","author":"Taylor","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ikeda, R., Lafor\u00eat, F., Antoine, C., Adachi, M., Nakamura, K., Habets, A., Kler, C., De Rauw, K., Hayashi, T., and Mainil, J.M. (2023). Virulence of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 in Galleria mellonella larvae: Comparison of the roles of the pS88 plasmids and STX2d phage. Vet. Sci., 10.","DOI":"10.3390\/vetsci10070420"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Menge, C. (2020). The role of Escherichia coli Shiga toxins in STEC colonization of cattle. Toxins, 12.","DOI":"10.3390\/toxins12090607"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1128\/JCM.42.2.645-651.2004","article-title":"Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene (eae-xi)","volume":"42","author":"Blanco","year":"2004","journal-title":"J. Clin. Microbiol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/jam.13677","article-title":"Low prevalence of the \u201cgang of seven\u201d and absence of the O80:H2 serotypes among Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) in intestinal contents of healthy cattle at two slaughterhouses in Belgium in 2014","volume":"124","author":"Thiry","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Habets, A., Engelen, F., Duprez, J.-N., Devleesschauwer, B., Heyndrickx, M., De Zutter, L., Thiry, D., Cox, E., and Mainil, J. (2020). Identification of Shigatoxigenic and enteropathogenic Escherichia coli serotypes in healthy young dairy calves in Belgium by recto-anal mucosal swabbing. Vet. Sci., 7.","DOI":"10.3390\/vetsci7040167"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1128\/IAI.73.3.1735-1743.2005","article-title":"Identification of enterohemorrhagic Escherichia coli O26:H- genes required for intestinal colonization in calves","volume":"73","author":"Dziva","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1128\/AEM.02893-06","article-title":"Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo","volume":"73","author":"Girard","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ray, R., and Singh, P. (2022). Prevalence and implications of Shiga toxin-producing E. coli in farm and wild ruminants. Pathogens, 11.","DOI":"10.3390\/pathogens11111332"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1128\/IAI.68.3.1400-1407.2000","article-title":"Iha: A novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structure","volume":"68","author":"Tarr","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"EHEC-0003-2013","DOI":"10.1128\/microbiolspec.EHEC-0003-2013","article-title":"Enterohemorrhagic Escherichia coli adhesins","volume":"2","author":"McWilliams","year":"2014","journal-title":"Microbiol. Spectrum"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"17043","DOI":"10.1073\/pnas.262441699","article-title":"Genomic diversity of enterohemorrhagic Escherichia coli O157 revealed by whole genome PCR scanning","volume":"99","author":"Ohnishi","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ogura, Y., Ooka, T., Asadulghani, M., Terajima, J., Nougayr\u00e8de, J.-P., Kurokawa, K., Tashiro, K., Tobe, T., Nakayama, K., and Kuhara, S. (2007). Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes. Genome Biol., 8.","DOI":"10.1186\/gb-2007-8-7-r138"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1111\/j.1365-2958.2010.07499.x","article-title":"Chromosomal instability in enterohaemorrhagic Escherichia coli O157:H7: Impact on adherence, tellurite resistance and colony phenotype","volume":"79","author":"Bielaszewska","year":"2011","journal-title":"Mol. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Lorenz, S.C., Gonzalez-Escalona, N., Kotewicz, M.L., Fischer, M., and Kase, J.A. (2017). Genome sequencing and comparative genomics of enterohemorrhagic Escherichia coli O145:H25 and O145:H28 reveal distinct evolutionary paths and marked variations in traits associated with virulence & colonization. BMC Microbiol., 17.","DOI":"10.1186\/s12866-017-1094-3"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ijfoodmicro.2011.01.041","article-title":"Characteristics of Shiga toxin-producing Escherichia coli from meat and milk products of different origins and association with food producing animals as main contamination sources","volume":"146","author":"Martin","year":"2011","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.ijfoodmicro.2012.09.016","article-title":"Detection and characterization of Shiga toxin-producing Escherichia coli in game meat and ready-to-eat meat products","volume":"160","author":"Hanning","year":"2012","journal-title":"Int. J. Food Microbiol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/3\/274\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:53:46Z","timestamp":1760028826000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/3\/274"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,14]]},"references-count":39,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["vetsci12030274"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12030274","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2025,3,14]]}}}