{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:04:48Z","timestamp":1789643088268,"version":"4.0.0"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:00:00Z","timestamp":1741996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2024YFD1800200"],"award-info":[{"award-number":["2024YFD1800200"]}]},{"name":"Research and Application of Integrated Prevention, Control and Purification Technologies for Major Swine Epidemic Diseases","award":["2024YFD1800500"],"award-info":[{"award-number":["2024YFD1800500"]}]},{"name":"Sichuan Province \u201c14th Five-Year Plan\u201d major scientific and technological specialization of Sichuan hogs","award":["2021ZDZX0010-3"],"award-info":[{"award-number":["2021ZDZX0010-3"]}]},{"name":"National agricultural industrial technology system Sichuan veterinary medicine innovation team special"},{"name":"Sichuan Science and Technology Program Projects","award":["2023YFN0021"],"award-info":[{"award-number":["2023YFN0021"]}]},{"name":"Sichuan Science and Technology Program","award":["2022YFN0007"],"award-info":[{"award-number":["2022YFN0007"]}]},{"name":"Chongqing Municipal Technology Innovation and Application Development Special Program","award":["cstc2021jscx-dxwtBX0007"],"award-info":[{"award-number":["cstc2021jscx-dxwtBX0007"]}]},{"name":"Strategic Priority Research Program of the National Center of Technology Innovation for Pigs","award":["NCTIP-XD\/C14"],"award-info":[{"award-number":["NCTIP-XD\/C14"]}]},{"name":"Sichuan Pig Innovation Team of the National Modern Agricultural Industry Technology System","award":["sccxtd-2024-08-15"],"award-info":[{"award-number":["sccxtd-2024-08-15"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Following its initial documented emergence in Hunan\u2019s swine facilities in 2017, GETV has inflicted considerable financial damage upon China\u2019s pork production sector. Beyond its impact on swine, GETV also poses a potential risk to other animal species and public health, primarily due to its mosquito-borne transmission. Between September 2020 and May 2021, a GETV outbreak occurred on a commercial swine farm in Sichuan Province, where affected animals exhibited movement disorders, neurological symptoms, and mild diarrhea, with severe cases resulting in mortality. To elucidate the genetic characteristics and transmission dynamics of the virus, a comprehensive investigation of this outbreak was conducted. Clinical samples from diseased or deceased swine were collected and analyzed by PCR, confirming the presence of GETV infection. Additionally, differential diagnostic assays were performed for other common swine pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), and atypical porcine pestivirus (APPV). All tests yielded negative results, confirming GETV as the sole etiological agent of the outbreak. The GETV-positive samples were inoculated into BHK-21 cells, leading to the isolation of a GETV strain (SC202009). Whole-genome sequence analysis revealed that SC202009 belongs to the GIII genotype of GETV, with a nucleotide sequence identity ranging from 95.1% to 99.8% compared to reference sequences in GenBank. This high level of sequence identity suggests that SC202009 is closely related to other circulating strains in the region, indicating potential local transmission networks and endemic viral circulation. The nucleotide sequence identity of the E2 gene ranged from 94.2% to 99.6%, while the E2 protein amino acid sequence identity ranged from 97.2% to 99.5%. Phylogenetic analysis further indicated that SC202009 is genetically distant from the original Malaysian prototype strain (MM2021) but shares high sequence identity with several strains isolated in Sichuan Province, including SC266, SC201807, and SC483. Notably, NMJA_F2_18-8L-NH-Cxp-Y-1-1 was isolated from Culex pipiens, suggesting a mosquito-borne transmission route. These findings provide novel insights into the regional dissemination patterns and genetic diversity of GETV in China. These results also highlight the importance of the continuous surveillance of viral genetic variations and the elucidation of transmission dynamics to develop effective control strategies and mitigate potential public health risks. Future studies should focus on the ecological and environmental factors influencing GETV transmission, particularly the role of mosquito vectors in cross-species viral spread, to enhance targeted prevention and control measures for this emerging arboviral threat.<\/jats:p>","DOI":"10.3390\/vetsci12030276","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T06:36:23Z","timestamp":1742193383000},"page":"276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Isolation and Characterization of a Porcine Getah Virus Strain from Sichuan Province"],"prefix":"10.3390","volume":"12","author":[{"given":"Lina","family":"Shao","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"given":"Lishuang","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Sichuan Key Laboratory of Animal Epidemic Disease and Human Health, College of Veterinary Medicine, 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