{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:11:17Z","timestamp":1760058677019,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["Finaced code 001"],"award-info":[{"award-number":["Finaced code 001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>(1): Background: This study aimed to evaluate the concentrations of four proteins for allergic patch testing (APT) in dogs, assessing sensitivity (SE), specificity (SP), negative predictive value (NPV), positive predictive value (PPV), reactions to adhesives\/containers, and the safety of APT with food proteins in dogs. (2) Methods: For evaluation, 43 dogs were screened and divided into two groups: Group 1 consisted of 20 healthy dogs, and Group 2 included 23 dogs with canine atopic dermatitis (AD). Group 1 underwent allergic patch testing (APT) with beef, pork, chicken, and soy proteins at four different concentrations (100 mg, 250 mg, 500 mg, 1000 mg\/0.2 mL). Of the 23 dogs included in Group 2, four did not undergo the elimination diet and were excluded, leaving 17 dogs in the study. They underwent an elimination diet (ED) and were evaluated using the pruritus visual analog scale (pVAS) and lesion scores (CADESI-4) before and after the ED (days 0 and 45). After the ED, Group 2 was subjected to APT (using the same proteins and concentrations as Group 1) and an oral provocation test (OPT) with the proteins used in the APT. The results of the OPT were used to assess the accuracy of the APT. (3) Results: In Group 1, one dog reacted to the APT. In Group 2, after 45 days of ED, of the 17 dogs included, 13 showed a reduction in pVAS and CADESI-4 scores (p &lt; 0.05) and nine an improvement considered good to excellent. Of these, two showed irritant contact reactions to the APT chambers and were excluded, leaving 11 dogs that were reactive to APT, and the OPT increased pruritus (p &lt; 0.05). Accuracy: Beef and chicken proteins at concentrations of 500 and 1000 mg\/0.2 mL, and soy protein at 1000 mg\/0.2 mL, achieved 100% SE, SP, PPV, and NPV. Pork protein at 1000 mg\/0.2 mL achieved 100% SE, 83% SP, 83% PPV, and 100% NPV. (4) Conclusions: APT with beef and chicken proteins at 500 mg and 1000 mg\/0.2 mL and soy protein at 1000 mg\/0.2 mL, based on the results of this study, can be recommended for diagnosing adverse food reactions in dogs with AD.<\/jats:p>","DOI":"10.3390\/vetsci12040383","type":"journal-article","created":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T20:24:16Z","timestamp":1745180656000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Standardization of Beef, Pork, Chicken, and Soy Protein Extracts for Patch Testing and Their Accuracy in Diagnosing Adverse Food Reactions in Dogs with Chronic Pruritus"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-2183","authenticated-orcid":false,"given":"Raniere","family":"Gaertner","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9102-1066","authenticated-orcid":false,"given":"Vanessa Cunningham","family":"Gmyterco","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3358-4826","authenticated-orcid":false,"given":"J\u00falia S\u00f3","family":"Severo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-8366","authenticated-orcid":false,"given":"Camilla","family":"Alcal\u00e1","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9156-0040","authenticated-orcid":false,"given":"Maicon Roberto","family":"Paulo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2331-1648","authenticated-orcid":false,"given":"Ruan","family":"Daros","sequence":"additional","affiliation":[{"name":"EthoLab\u2014Applied Ethology and Animal Welfare Lab, Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-1767","authenticated-orcid":false,"given":"Marconi Rodrigues","family":"de Farias","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, School of Medicine and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, 1155 Imaculada Concei\u00e7\u00e3o Street, Curitiba 80215-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"408-e134","DOI":"10.1111\/vde.12680","article-title":"An open label clinical trial to evaluate the utility of a hydrolysed fish and rice starch elimination diet for the diagnosis of adverse food reactions in dogs","volume":"29","author":"Matricoti","year":"2018","journal-title":"Vet. 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