{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:13:42Z","timestamp":1784758422428,"version":"3.55.0"},"reference-count":61,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RD&amp;E","award":["FDA-CO-2558-1603-TH"],"award-info":[{"award-number":["FDA-CO-2558-1603-TH"]}]},{"name":"National Science and Technology Development Agency of Thailand (NSTDA)","award":["FDA-CO-2558-1603-TH"],"award-info":[{"award-number":["FDA-CO-2558-1603-TH"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The use of cassava as a feed resource is the main focus of strategies that aim to increase the efficiency of beef cattle. In this study, the ratio of fresh cassava tops to roots (CTR) was 1:1, 1:2, 1:3, and 1:4 w\/w, in addition to the incorporation of yeast and lactic acid bacteria (YL). CTRS was inoculated in vitro with rumen fluid and a substrate mixture to evaluate the gas production and fermentation quality. In vivo, crossbred beef cattle were randomly allocated to three dietary treatments: a control group and two groups that received total mixed ration (TMR) silage with either 35% or 70% of the concentrate replaced with CTRS. This study found that the levels of crude protein and condensed tannins (CTs) in CTRS were relatively increased by the addition of cassava tops (p &lt; 0.05), while the fermentation pH decreased as the proportion of cassava roots increased. CTRS significantly enhanced in vitro dry matter digestibility, cumulative gas at 96 h, gas production kinetics, and MBP (p &lt; 0.05), especially in the 1:2 group. In the in vivo experiment, CTRS increased DM intake, CP, and propionic acid concentration compared to the control group and showed great potential for improving NDF digestibility. Moreover, when the concentrate in the TMR silage was substituted with CTRS and offered to crossbred beef cattle, it increased ruminal NH3-N and blood glucose levels (p &lt; 0.05). The TMR silage substituted with 70% CTRS reduced the feed cost by 50.54% (p &lt; 0.05). Our study findings suggest that optimizing the inclusion of CTRS in TMR silage could be a viable strategy for beef producers aiming to enhance cattle performance and profitability.<\/jats:p>","DOI":"10.3390\/vetsci12050402","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T10:17:16Z","timestamp":1745489836000},"page":"402","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inclusion of Fermented Cassava Top and Root with Inoculation in Total Mixed Ration Silage Diets: Ensilage Quality and Nutrient Digestibility of Backgrounding Crossbred Bulls"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4335-9916","authenticated-orcid":false,"given":"Pichad","family":"Khejornsart","sequence":"first","affiliation":[{"name":"Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unchan","family":"Traithilen","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theerayut","family":"Juntanam","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1338299","DOI":"10.3389\/fsufs.2024.1338299","article-title":"Crop\u2013livestock-integrated farming system: A strategy to achieve synergy between agricultural production, nutritional security, and environmental sustainability","volume":"8","author":"Shanmugam","year":"2004","journal-title":"Front. Sustain. Food Syst."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100295","DOI":"10.1016\/j.animal.2021.100295","article-title":"An overview of beef production from pasture and feedlot globally, as demand for beef and the need for sustainable practices increase","volume":"15","author":"Greenwood","year":"2021","journal-title":"Animal"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1139\/cjas-2020-0022","article-title":"Strategies to improve the efficiency of beef cattle production","volume":"101","author":"Terry","year":"2021","journal-title":"Can. J. Anim. Sci."},{"key":"ref_4","unstructured":"Alexandratos, N., and Bruinsma, J. (2012). World Agriculture Towards 2030\/2050: The 2012 Revision, FAO. ESA Working paper No. 12-03."},{"key":"ref_5","unstructured":"FAO (2025, February 27). World Food and Agriculture\u2014Statistical Yearbook 2024. Rome. Available online: https:\/\/openknowledge.fao.org\/handle\/20.500.14283\/cd2971en."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.aninu.2015.12.001","article-title":"Cassava chip (Manihot esculenta Crantz) as an energy source for ruminant feeding","volume":"1","author":"Wanapat","year":"2015","journal-title":"Anim. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"754","DOI":"10.5455\/javar.2022.i645","article-title":"Cassava pulp added to fermented total mixed rations increased tropical sheep\u2019s nutrient utilization, rumen ecology, and microbial protein synthesis","volume":"9","author":"Khejornsart","year":"2022","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lambebo, T., and Deme, T. (2022). Evaluation of nutritional potential and effect of processing on improving nutrient content of cassava (Manihot esculenta Crantz) root and leaves. bioRxiv.","DOI":"10.1101\/2022.02.04.479097"},{"key":"ref_9","first-page":"952","article-title":"Effect of carbohydrate source and cottonseed meal level in the concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes","volume":"26","author":"Wanapat","year":"2013","journal-title":"Anim. Biosci."},{"key":"ref_10","first-page":"504","article-title":"The role of cassava hay as animal feed","volume":"7","author":"Wanapat","year":"2002","journal-title":"Dep. Agric. (DOA) Cent. Int. De Agric. Trop. (CIAT)"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100675","DOI":"10.1016\/j.focha.2024.100675","article-title":"Cassava waste (stem and leaf) analysis for reuse","volume":"4","author":"Soares","year":"2024","journal-title":"Food Chem. Adv."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s11250-021-02656-9","article-title":"Enhancement values of cassava by-product diets on production and haemato-biochemical indices of sheep and goats: A review","volume":"53","author":"Jiwuba","year":"2021","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"561","DOI":"10.56899\/152.02.03","article-title":"Effect of processing on the antinutrient and protein contents of cassava leaves from selected varieties","volume":"152","author":"Tambalo","year":"2023","journal-title":"Philipp. J. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Prachumchai, R., Cherdthong, A., Wanapat, M., So, S., and Polyorach, S. (2022). Fresh cassava root replacing cassava chip could enhance milk production of lactating dairy cows fed diets based on high sulfur-containing pellet. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-07825-w"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Supapong, C., Sommai, S., Khonkhaeng, B., Suntara, C., Prachumchai, R., Phesatcha, K., Chanjula, P., and Cherdthong, A. (2022). Effect of rhodanese enzyme addition on rumen fermentation, cyanide concentration, and feed utilization in beef cattle receiving various levels of fresh cassava root. Fermentation, 8.","DOI":"10.3390\/fermentation8040146"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1080\/1828051X.2024.2445766","article-title":"Effects of cassava pulp fermentation with traditional starter media on rumen fermentation, nutrients digestibility in beef cattle","volume":"24","author":"Khejornsart","year":"2025","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_17","first-page":"262","article-title":"Enriching the nutritive value of cassava as feed to increase ruminant productivity","volume":"1","author":"Wanapat","year":"2013","journal-title":"Nutr. Ecol. Food Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.5713\/ab.24.0076","article-title":"Effect of replacing concentrates with cassava root-top silage on feed utilization, rumen fermentation, blood parameters and growth performance in beef cattle","volume":"37","author":"Gunun","year":"2024","journal-title":"Anim. Biosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s11250-013-0433-0","article-title":"Improvement of whole crop rice silage nutritive value and rumen degradability by molasses and urea supplementation","volume":"45","author":"Wanapat","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_20","first-page":"599","article-title":"Effect of type of treated rice straw on ruminal fermentation characteristic and cellilolytic bacteria of swamp buffalo","volume":"40","author":"Khejornsart","year":"2021","journal-title":"Buffalo Bull."},{"key":"ref_21","first-page":"7","article-title":"Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid","volume":"28","author":"Menke","year":"1988","journal-title":"Anim. Res. Dev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1017\/S0021859600063048","article-title":"The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage","volume":"92","author":"McDonald","year":"1979","journal-title":"J. Agric. Sci."},{"key":"ref_23","unstructured":"National Academies of Sciences, Engineering, and Medicine (2016). Nutrient Requirements of Beef Cattle, The National Academies Press. [8th ed.]."},{"key":"ref_24","unstructured":"Goering, H.K., and Van Soest, P.J. (1970). Forage Fiber Analyses (Apparatus, Reagents, Procedures, and Some Applications), USDA Agricultural Handbook No 379."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0301-6226(00)00241-4","article-title":"Predicting ruminal microbial efficiencies of dairy rations by in vitro techniques","volume":"68","author":"Blummel","year":"2001","journal-title":"Livest. Prod. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Franco, M., Tapio, I., Pirttiniemi, J., Stefa\u0144ski, T., Jalava, T., Huuskonen, A., and Rinne, M. (2022). Fermentation Quality and Bacterial Ecology of Grass Silage Modulated by Additive Treatments, Extent of Compaction and Soil Contamination. Fermentation, 8.","DOI":"10.3390\/fermentation8040156"},{"key":"ref_27","unstructured":"AOAC (2016). Official Method of Analysis, Association of Official Analytical Chemists. [20th ed.]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","article-title":"Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition","volume":"74","author":"Robertson","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1002\/jsfa.2740580306","article-title":"Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains","volume":"58","author":"Terril","year":"1992","journal-title":"J. Sci. Food Agric."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5295","DOI":"10.3168\/jds.2012-6442","article-title":"Short communication: Evaluation of acid-insoluble ash and indigestible neutral detergent fiber as total-tract digestibility markers in dairy cows fed corn silage-based diets","volume":"96","author":"Lee","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Jur\u00e1\u0161kov\u00e1, D., Ribeiro, S.C., and Silva, C.C.G. (2022). Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties. Foods, 11.","DOI":"10.3390\/foods11020156"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.2527\/jas.2014-8287","article-title":"Impact of adding Saccharomyces strains on fermentation, aerobic stability, nutritive value, and select lactobacilli populations in corn silage","volume":"93","author":"Duniere","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_33","first-page":"387","article-title":"Factors affecting the utilisation of cassava products for poultry feeding review","volume":"52","author":"Devi","year":"2021","journal-title":"Egypt J. Vet. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"88","DOI":"10.4314\/as.v22i3.12","article-title":"Nutritional assay and growth performance of West African dwarf goats fed with fresh, wilted and ensiled cassava leaves-based diet","volume":"22","author":"Olalere","year":"2023","journal-title":"Agro-Science"},{"key":"ref_35","first-page":"1219","article-title":"The chemical composition, anti-nutritional and microbial properties of ensiled cassava root-leaf blends as potential feed in swine diet","volume":"42","author":"Amos","year":"2019","journal-title":"Pertanika J. Trop. Agric. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"173","DOI":"10.12691\/ajfst-9-4-9","article-title":"Lactic Acid Bacteria and Yeasts Associated with Cassava Fermentation to atti\u00e9k\u00e9 in Burkina Faso and Their Technological Properties","volume":"9","author":"Flibert","year":"2021","journal-title":"Am. J. Adv. Food Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"012004","DOI":"10.1088\/1757-899X\/1011\/1\/012004","article-title":"Fermentation of amylolytic yeast and lactic acid bacteria to improve the quality of modified cassava","volume":"1011","author":"Damayanti","year":"2021","journal-title":"IOP Conf. Ser. Mater. Sci. Eng."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rashid, M., Aboshady, H.M., Soltan, Y.A., Archimede, H., and Ghoneem, W.M.A. (2025). Phytochemical profile and in vitro evaluation of cassava (Manihot esculenta Crantz) foliage as ruminant feed with\/without green banana flour. Sci. Rep., 15.","DOI":"10.1038\/s41598-024-82450-3"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"211","DOI":"10.14202\/vetworld.2019.211-217","article-title":"Reduction of proteolysis of high protein silage from Moringa and Indigofera leaves by addition of tannin extract","volume":"12","author":"Jayanegara","year":"2019","journal-title":"Vet. World"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6142","DOI":"10.3168\/jds.2017-13944","article-title":"Short communication: In vitro rumen gas production and starch degradation of starch-based feeds depend on mean particle size","volume":"101","author":"Gallo","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1002\/jsfa.9406","article-title":"Ensiling characteristics, in vitro rumen fermentation, microbial communities and aerobic stability of low-dry matter silages produced with sweet sorghum and alfalfa mixtures","volume":"99","author":"Chen","year":"2019","journal-title":"J. Sci. Food Agric."},{"key":"ref_42","first-page":"75","article-title":"Survey of the in vitro nutritional value of total mixed rations silage with fresh chopped alfalfa forage at two levels of dry matter and crude protein","volume":"12","author":"Dayani","year":"2024","journal-title":"J. Rumin. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/jsfa.1021","article-title":"In vitro degradability of three forages: Fermentation kinetics and gas production of NDF and neutral detergent-soluble fraction of forages","volume":"82","author":"Infascelli","year":"2002","journal-title":"J. Sci. Food Agric."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1002\/jsfa.11643","article-title":"Influence of Microbial Probiotics on Ruminant Health and Nutrition: Sources, Mode of Action and Implications","volume":"102","author":"Reuben","year":"2022","journal-title":"J. Sci. Food Agric."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.2527\/1995.7392774x","article-title":"Characteristics of plant cell walls affecting intake and digestibility of forages by ruminants","volume":"73","author":"Jung","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1007\/s11250-024-03962-8","article-title":"Effects of supplementing cassava root silage to grazing dairy cows on nutrient utilization, milk production and composition in the tropics","volume":"56","author":"Vargas","year":"2024","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Khejornsart, P., Juntanam, T., Gunun, P., Gunun, N., and Cherdthong, A. (2024). Effect of High-Tannin and -Polyphenol Plant Material Supplement on Rumen Fermentation, Nitrogen Partitioning and Nutrient Utilization in Beef Cattle. Animals, 14.","DOI":"10.3390\/ani14213092"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1071\/EA07199","article-title":"Nutritional management for enteric methane abatement: A review","volume":"48","author":"Beauchemin","year":"2008","journal-title":"Aust. J. Exp. Agric."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Avila, A.S., Zambom, M.A., Faccenda, A., Fischer, M.L., Anschau, F.A., Venturini, T., Tinini, R.C.R., Dessbesell, J.G., and Faciola, A.P. (2020). Effects of Black Wattle (Acacia mearnsii) Condensed Tannins on Intake, Protozoa Population, Ruminal Fermentation, and Nutrient Digestibility in Jersey Steers. Animals, 10.","DOI":"10.3390\/ani10061011"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Besharati, M., Maggiolino, A., Palangi, V., Kaya, A., Jabbar, M., Eseceli, H., De Palo, P., and Lorenzo, J.M. (2022). Tannin in Ruminant Nutrition: Review. Molecules, 27.","DOI":"10.3390\/molecules27238273"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0377-8401(01)00232-2","article-title":"Microbial interactions with tannins: Nutritional consequences for ruminants","volume":"91","author":"McSweeney","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.1365-2494.2004.00427.x","article-title":"Modelling the comparative productivity and profitability of grass and legume systems of silage production in northern Europe","volume":"59","author":"Topp","year":"2004","journal-title":"Grass Forage Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e35254","DOI":"10.1016\/j.heliyon.2024.e35254","article-title":"Economically optimized forage utilization choices in drylands for adapting to economic, ecological, and climate stress","volume":"10","author":"Trail","year":"2024","journal-title":"Heliyon"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kim, D., Jung, J.-S., and Choi, K.-C. (2021). A Preliminary Study on Effects of Fermented Feed Supplementation on Growth Performance, Carcass Characteristics, and Meat Quality of Hanwoo Steers during the Early and Late Fattening Period. Appl. Sci., 11.","DOI":"10.3390\/app11115202"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.3168\/jds.S0022-0302(06)72395-5","article-title":"Effects of dairy cow diet forage proportion on duodenal nutrient supply and urinary purine derivative excretion","volume":"89","author":"Moorby","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Gunun, N., Khejornsart, P., Polyorach, S., Kaewpila, C., Kimprasit, T., Sanjun, I., Cherdthong, A., Wanapat, M., and Gunun, P. (2022). Utilization of Mao (Antidesma thwaitesianum Muell. Arg.) Pomace Meal to Substitute Rice Bran on Feed Utilization and Rumen Fermentation in Tropical Beef Cattle. Vet. Sci., 9.","DOI":"10.3390\/vetsci9110585"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Hackmann, T.J., and Firkins, J.L. (2015). Maximizing efficiency of rumen microbial protein production. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00465"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0377-8401(03)00041-5","article-title":"The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: A review","volume":"106","author":"Min","year":"2003","journal-title":"Animal Feed Sci. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4398","DOI":"10.1093\/jas\/sky273","article-title":"Effect of feeding condensed tannins in high protein finishing diets containing corn distillers grains on ruminal fermentation, nutrient digestibility, and route of nitrogen excretion in beef cattle","volume":"96","author":"Koenig","year":"2018","journal-title":"J. Animal Sci."},{"key":"ref_60","first-page":"120","article-title":"Advancements in rumen microbiology: Implications for livestock health","volume":"47","author":"Jones","year":"2020","journal-title":"J. Vet. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"101319","DOI":"10.1016\/j.animal.2024.101319","article-title":"Animal board invited review: The effect of diet on rumen microbial composition in dairy cows","volume":"18","author":"Palmonari","year":"2021","journal-title":"Animal"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/5\/402\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:21:01Z","timestamp":1760030461000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/5\/402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":61,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["vetsci12050402"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12050402","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]}}}