{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:18:48Z","timestamp":1779333528867,"version":"3.51.4"},"reference-count":57,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Silvateam USA, New York, NY"},{"name":"Gordon W. Davis Regents Chair Endowment at Texas Tech University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study evaluates the effects of a proprietary tannin blend (BX), supplemented with or without sodium monensin (MON), on beef cattle performance, carcass traits, and health. Steers (n = 2986; initial shrunk body weight (SBW) 254 \u00b1 9.2 kg) were allocated into 48 pens (61\u201362 steers\/pen; 12 pens\/treatment) fed for 230 d. Treatments included: (1) no feed additives (CTL); (2) BX (7.95 g\/animal daily); (3) MON + Tylosin phosphate (TYL; 437.52 mg MON\/animal daily + 80 mg TYL\/animal daily); or (4) MON + BX (437.52 mg MON\/animal daily + 7.95 g BX\/animal daily). Data were analyzed in R 4.2.1 using a randomized block design with pen as the experimental unit. Dry matter intake was lower (p &lt; 0.001) in MON + TYL and MON + BX than in CTL. Steers fed MON + BX had greater carcass-adjusted final SBW (p = 0.002), average daily gain (p = 0.002), fat thickness (p = 0.035), and marbling score (p = 0.046) than BX. Feed conversion improved in MON + TYL and MON + BX (p &lt; 0.001). CLT and BX had higher (p &lt; 0.001) liver abscess prevalence compared to MON + BX and MON + TYL. The addition of BX did not improve the parameters measured. Steers fed MON + BX showed reduced liver abscesses and similar feed efficiency compared to MON + TYL.<\/jats:p>","DOI":"10.3390\/vetsci12050446","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T09:08:56Z","timestamp":1746522536000},"page":"446","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating a Proprietary Tannin-Blend Product as an Alternative to Monensin and Tylosin Phosphate in Feedlot Cattle Diets"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1527-1079","authenticated-orcid":false,"given":"Luana D.","family":"Felizari","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9086-3322","authenticated-orcid":false,"given":"Luke K.","family":"Fuerniss","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan L.","family":"Beckett","sequence":"additional","affiliation":[{"name":"Beckett Consulting Services, Fort Collins, CO 80524, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Secrist","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy D.","family":"Hufstedler","sequence":"additional","affiliation":[{"name":"Silvateam USA, New York, NY 10022, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8445-2578","authenticated-orcid":false,"given":"Bradley J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: A review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.2527\/jas.2015-0261","article-title":"Liver abscesses in cattle: A review of incidence in Holsteins and of bacteriology and vaccine approaches to control in feedlot cattle","volume":"94","author":"Amachawadi","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"287","DOI":"10.2527\/1998.761287x","article-title":"Liver abscesses in feedlot cattle: A review","volume":"76","author":"Nagaraja","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4583","DOI":"10.2527\/jas.2011-5018","article-title":"Meta-analysis of the effects of monensin in beef cattle on feed efficiency, body weight gain, and dry matter intake","volume":"90","author":"Duffield","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.2527\/jas.2016-0282","article-title":"Nutritional recommendations of feedlot consulting nutritionists: The 2015 New Mexico State and Texas Tech University survey","volume":"94","author":"Samuelson","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1080\/09712119.2023.2191679","article-title":"Adoption of adaptation protocols and feed additives to improve performance of feedlot cattle","volume":"51","author":"Silvestre","year":"2023","journal-title":"J. Appl. Anim. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.cvfa.2007.05.002","article-title":"Liver abscesses in feedlot cattle","volume":"23","author":"Nagaraja","year":"2007","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_8","unstructured":"USDA\u2013APHIS\u2013VS\u2013CEAH\u2013NAHMS (2025, January 15). Feedlot 2011 Part IV: Health and Health Management on U.S. Feedlots with a Capacity of 1,000 or More Head. USDA, Fort Collins, CO, 2013, Available online: https:\/\/www.aphis.usda.gov\/animal_health\/nahms\/feedlot\/downloads\/feedlot2011\/Feed11_dr_PartIV.pdf."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.2527\/jas.2009-1778","article-title":"Analysis of modern technologies commonly used in beef cattle production: Conventional beef production versus nonconventional production using meta-analysis","volume":"87","author":"Wileman","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_10","unstructured":"OJEU (2025, February 10). Regulation (EC) No 1831\/2003 of the European Parliament and the Council of September 22, 2003, on Additives for Use in Animal Nutrition. Official Journal of European Union, 2003, L268-36 in OJEU of 10\/18\/2003. Available online: http:\/\/data.europa.eu\/eli\/reg\/2003\/1831\/oj."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6968.2008.01145.x","article-title":"Update on macrolide-lincosamide-streptogramin, ketolide, and oxazolidinone resistance genes","volume":"282","author":"Roberts","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_12","unstructured":"World Health Organization (2017). WHO Guidelines on Use of Medically Important Antimicrobials in Food-Producing Animals, World Health Organization."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Besharati, M., Maggiolino, A., Palangi, V., Kaya, A., Jabbar, M., Eseceli, H., De Palo, P., and Lorenzo, J.M. (2022). Tannin in Ruminant Nutrition: Review. Molecules, 27.","DOI":"10.3390\/molecules27238273"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1002\/jsfa.2577","article-title":"Unraveling the conundrum of tannins in animal nutrition and health","volume":"86","year":"2006","journal-title":"J. Sci. Food Agric."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.aninu.2017.09.004","article-title":"Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production","volume":"4","author":"Huang","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Orzuna-Orzuna, J.F., Dorantes-Iturbide, G., Lara-Bueno, A., Mendoza-Martinez, G.D., Miranda-Romero, L.A., and Hernandez-Garcia, P.A. (2021). Effects of dietary tannins supplementation on growth performance, rumen fermentation, and enteric methane emissions in beef cattle: A meta-analysis. Sustainability, 13.","DOI":"10.3390\/su13137410"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Adejoro, F.A., Hassen, A., and Akanmu, A.M. (2019). Effect of Lipid-Encapsulated Acacia Tannin Extract on Feed Intake, Nutrient Digestibility and Methane Emission in Sheep. Animals, 9.","DOI":"10.3390\/ani9110863"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"114217","DOI":"10.1016\/j.anifeedsci.2019.114217","article-title":"Effects of dietary supplementation with Pinus taeda hydrolyzed lignin on in vivo performances, in vitro nutrient apparent digestibility, and gas emission in beef steers","volume":"255","author":"Maggiolino","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ciliberti, M.G., Albenzio, M., Francavilla, M., Neglia, G., Esposito, L., and Caroprese, M. (2019). Extracts from Microalga Chlorella sorokiniana Exert an Anti-Proliferative Effect and Modulate Cytokines in Sheep Peripheral Blood Mononuclear Cells. Animals, 9.","DOI":"10.3390\/ani9020045"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e09828","DOI":"10.1016\/j.heliyon.2022.e09828","article-title":"Modulatory effects of dietary tannins on polyunsaturated fatty acid biohydrogenation in the rumen: A meta-analysis","volume":"29","author":"Makmur","year":"2022","journal-title":"Heliyon"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, J., and Monje-Galvan, V. (2023). In Vitro and In Silico Studies of Antimicrobial Saponins: A Review. Processes, 11.","DOI":"10.3390\/pr11102856"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kholif, A.E. (2023). A Review of Effect of Saponins on Ruminal Fermentation, Health and Performance of Ruminants. Vet. Sci., 10.","DOI":"10.3390\/vetsci10070450"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"464","DOI":"10.15232\/S1080-7446(15)30386-7","article-title":"Effects of Plant Tannin Supplementation on Animal Responses and In Vivo Ruminal Bacterial Populations Associated with Bloat in Heifers Grazing Wheat Forage","volume":"28","author":"Min","year":"2012","journal-title":"Prof. Anim. Sci."},{"key":"ref_24","first-page":"163","article-title":"Effect of a Blend of Essential Oils, Bioflavonoids, and Tannins on Production Performance, Health, Immune Functionality, and Antioxidant Status in Fattening Beef Cattle","volume":"29","author":"Grossi","year":"2023","journal-title":"Large Anim. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"txae136","DOI":"10.1093\/tas\/txae136","article-title":"Growth performance and carcass characteristics of bulls fed tannins associated with or not with monensin","volume":"8","author":"Nascimento","year":"2024","journal-title":"Transl. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/09712119.2016.1141776","article-title":"Effect of level and source of supplemental tannin on growth performance of steers during the late finishing phase","volume":"45","author":"Plascencia","year":"2017","journal-title":"J. Appl. Anim. Res."},{"key":"ref_27","first-page":"600","article-title":"Effect of tannin extracts supplementation on feedlot performance and plasma urea nitrogen of yearling bulls fed dry-ground corn-based diets containing corn-DDG and cane molasses","volume":"90","author":"Barajas","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1093\/jas\/skae234.860","article-title":"Proteic dry supplements enriched with a blend of tannin-based extract enhance the performance of grass-fed cattle during the rainy season in tropical regions","volume":"102","author":"Manella","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"skae087","DOI":"10.1093\/jas\/skae087","article-title":"Influence of low-level tannin supplementation on comparative growth performance of Holstein and Angus \u00d7 Holstein cross calf-fed concentrate-based finishing diets for 328 d","volume":"102","author":"Carvalho","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2010.05.003","article-title":"Effects of dietary tannin source on performance, feed efficiency, ruminal fermentation, and carcass and non-carcass traits in steers fed a high-grain diet","volume":"159","author":"Krueger","year":"2010","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.2527\/jas2017.1464","article-title":"Effects of tannic acid on growth performance, carcass characteristics, digestibility, nitrogen volatilization, and meat lipid oxidation of steers fed steam-flaked corn-based finishing diets","volume":"95","author":"Tabke","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e20230104","DOI":"10.37496\/rbz5320230104","article-title":"Feedlot performance of Nellore bulls fed high-concentrate diets containing the association of tannins and saponins with sodium monensin","volume":"53","author":"Ferracini","year":"2024","journal-title":"Rev. Bras. Zootec."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bowman-Schnug, S.M., Fuerniss, L.K., Cameron, J.D., Beckett, J.L., Ahsin, M., van Vliet, S., Hufstedler, G.D., and Johnson, B.J. (2025). Replacement of Monensin with a Proprietary Tannin-Blend Additive in Calf-Fed Holstein Steer Diets. Vet. Sci., 12.","DOI":"10.3390\/vetsci12020166"},{"key":"ref_34","unstructured":"FASS (2010). Guide for Care and Use of Agricultural Animals in Agricultural Research and Teaching, Federation of Animal Science Society."},{"key":"ref_35","unstructured":"USDA AMS (2025, January 15). United States Standards for Grades of Carcass Beef, Available online: https:\/\/www.ams.usda.gov\/sites\/default\/files\/media\/CarcassBeefStandard.pdf."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0921-4488(03)00142-1","article-title":"Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds","volume":"49","author":"Makkar","year":"2003","journal-title":"Small Rumin. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4398","DOI":"10.1093\/jas\/sky273","article-title":"Effect of feeding condensed tannins in high protein finishing diets containing corn distiller grains on ruminal fermentation, nutrient digestibility, and route of nitrogen excretion in beef cattle","volume":"96","author":"Koenig","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e13765","DOI":"10.1111\/asj.13765","article-title":"Divergence effects between dietary Acacia and Quebracho tannin extracts in nutrient utilization, performance, and methane emission of ruminants: A meta-analysis","volume":"93","author":"Fitri","year":"2022","journal-title":"Anim. Sci. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1021\/jf00123a006","article-title":"Tannins: Their adverse role in ruminant nutrition","volume":"32","author":"Kumar","year":"1984","journal-title":"J. Agric. Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"txad048","DOI":"10.1093\/tas\/txad048","article-title":"Tannin-based product in feedlot diet as a strategy to reduce enteric methane emissions of Nellore cattle finished under tropical conditions","volume":"7","author":"Magnani","year":"2023","journal-title":"Transl. Anim. Sci."},{"key":"ref_41","first-page":"89","article-title":"Use of tannin extract as a strategy to reduce methane in Nellore and Holstein cattle and its effect on intake, digestibility, microbial efficiency, and ruminal fermentation","volume":"107","author":"Nogueira","year":"2022","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.livsci.2017.06.009","article-title":"Effect of tannins-rich extract from Acacia mearnsii or monensin as feed additives on ruminal fermentation efficiency in cattle","volume":"203","author":"Cassiano","year":"2017","journal-title":"Livest. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4141\/CJAS08110","article-title":"Potential use of Acacia mearnsii condensed tannins to reduce methane emissions and nitrogen excretion from grazing dairy cows","volume":"89","author":"Grainger","year":"2009","journal-title":"Can. J. Anim. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1093\/jas\/skae234.877","article-title":"Use of a commercial blend of tannins as feed additive for feed lot cattle on large pen trial improves carcass weight and carcass feed conversion","volume":"102","author":"Manella","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_45","first-page":"1345","article-title":"Effect of condensed tannin extract supplementation on growth performance, nitrogen balance, gas emissions, and energetic losses of beef steers","volume":"95","author":"Ebert","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s00248-004-0180-x","article-title":"Bacterial Mechanisms to Overcome Inhibitory Effects of Dietary Tannins","volume":"50","author":"Smith","year":"2005","journal-title":"Microb. Ecol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.3168\/jds.2022-22521","article-title":"Effect of Low Dietary Concentrations of Acacia mearnsii Tannin Extract on Chewing, Ruminal Fermentation, Digestibility, Nitrogen Partition, and Performance of Dairy Cows","volume":"106","author":"Oliveira","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2307\/3899675","article-title":"Relationships Among Soluble Phenolics, Insoluble Proanthocyanidins and Fiber in East African Browse Species","volume":"39","author":"Reed","year":"1986","journal-title":"J. Range Manag."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"170","DOI":"10.15232\/aas.2021-02228","article-title":"Exploratory observational quantification of liver abscess incidence, specific to region and cattle type, and their associations to viscera value and bacterial flora","volume":"38","author":"Herrick","year":"2022","journal-title":"Appl. Anim. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"237","DOI":"10.15232\/aas.2023-02498","article-title":"Liver abscesses\u2014New perspectives on a historic fed-cattle issue","volume":"40","author":"Broadway","year":"2024","journal-title":"Appl. Anim. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Pinnell, L.J., Young, J.D., Thompson, T.W., Wolfe, C.A., Bryant, T.C., Nair, M.N., Richeson, J.T., and Morley, P.S. (2023). Establishing the link between microbial communities in bovine liver abscesses and the gastrointestinal tract. Anim. Microbiome, 5.","DOI":"10.1186\/s42523-023-00278-0"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Davedow, T., Narvaez-Bravo, C., Zaheer, R., Sanderson, H., Rodas-Gonzalez, A., Klima, C., Booker, C.W., Hannon, S.J., Bras, A.L., and Gow, S. (2020). Investigation of a reduction in tylosin on the prevalence of liver abscesses and antimicrobial resistance in enterococci in feedlot cattle. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00090"},{"key":"ref_53","first-page":"211","article-title":"Reduction of liver abscesses in feedlot cattle by the addition of tannins on diet","volume":"45","author":"Cabral","year":"2019","journal-title":"Rev. Investig. Agropecu."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.2527\/jas1985.6151058x","article-title":"Effect of Monensin and Tylosin on Average Daily Gain, Feed Efficiency and Liver Abscess Incidence in Feedlot Cattle","volume":"61","author":"Potter","year":"1985","journal-title":"J. Anim. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Choi, J., Liu, G., Goo, D., Wang, J., Bowker, B., Zhuang, H., and Kim, W.K. (2022). Effects of tannic acid supplementation on growth performance, gut health, and meat production and quality of broiler chickens raised in floor pens for 42 days. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.1082009"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Buyse, K., Noten, N.V., Delezie, E., Goethals, L., Janssens, G.P., and Louren\u00e7o, M. (2022). Chestnut tannins in broiler diets: Affecting intestinal development in different feeding phases. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.996524"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/jam.14948","article-title":"Efficacy of Chestnut and Quebracho Wood Extracts to Control Salmonella in Poultry","volume":"131","author":"Casanova","year":"2021","journal-title":"J. Appl. Microbiol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/5\/446\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:27:38Z","timestamp":1760030858000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/5\/446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,6]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["vetsci12050446"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12050446","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,6]]}}}