{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T18:36:00Z","timestamp":1778524560680,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Program of Shanxi Province","award":["202403021221077"],"award-info":[{"award-number":["202403021221077"]}]},{"name":"Fundamental Research Program of Shanxi Province","award":["RPJJ2020021"],"award-info":[{"award-number":["RPJJ2020021"]}]},{"name":"Fundamental Research Program of Shanxi Province","award":["2021BQ06"],"award-info":[{"award-number":["2021BQ06"]}]},{"name":"Fundamental Research Program of Shanxi Province","award":["YLXKZX-NND-012"],"award-info":[{"award-number":["YLXKZX-NND-012"]}]},{"name":"Research Fund (Clinical Diagnosis and Treatment of Pet) for Young College Teachers in Ruipeng Commonwealth Foundation","award":["202403021221077"],"award-info":[{"award-number":["202403021221077"]}]},{"name":"Research Fund (Clinical Diagnosis and Treatment of Pet) for Young College Teachers in Ruipeng Commonwealth Foundation","award":["RPJJ2020021"],"award-info":[{"award-number":["RPJJ2020021"]}]},{"name":"Research Fund (Clinical Diagnosis and Treatment of Pet) for Young College Teachers in Ruipeng Commonwealth Foundation","award":["2021BQ06"],"award-info":[{"award-number":["2021BQ06"]}]},{"name":"Research Fund (Clinical Diagnosis and Treatment of Pet) for Young College Teachers in Ruipeng Commonwealth Foundation","award":["YLXKZX-NND-012"],"award-info":[{"award-number":["YLXKZX-NND-012"]}]},{"name":"Project of Science and Technology Innovation Fund of Shanxi Agricultural University","award":["202403021221077"],"award-info":[{"award-number":["202403021221077"]}]},{"name":"Project of Science and Technology Innovation Fund of Shanxi Agricultural University","award":["RPJJ2020021"],"award-info":[{"award-number":["RPJJ2020021"]}]},{"name":"Project of Science and Technology Innovation Fund of Shanxi Agricultural University","award":["2021BQ06"],"award-info":[{"award-number":["2021BQ06"]}]},{"name":"Project of Science and Technology Innovation Fund of Shanxi Agricultural University","award":["YLXKZX-NND-012"],"award-info":[{"award-number":["YLXKZX-NND-012"]}]},{"name":"Inner Mongolia Autonomous Region First Class Discipline Research Special Project","award":["202403021221077"],"award-info":[{"award-number":["202403021221077"]}]},{"name":"Inner Mongolia Autonomous Region First Class Discipline Research Special Project","award":["RPJJ2020021"],"award-info":[{"award-number":["RPJJ2020021"]}]},{"name":"Inner Mongolia Autonomous Region First Class Discipline Research Special Project","award":["2021BQ06"],"award-info":[{"award-number":["2021BQ06"]}]},{"name":"Inner Mongolia Autonomous Region First Class Discipline Research Special Project","award":["YLXKZX-NND-012"],"award-info":[{"award-number":["YLXKZX-NND-012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Toxoplasma gondii is a globally significant zoonotic pathogen responsible for severe parasitic diseases in humans and animals. This study aimed to design, develop, and evaluate a novel immunochromatographic test (ICT) using a recombinant MIC2-MIC3 fusion protein (rMIC2-MIC3) for detecting specific antibodies against T. gondii. The ICT demonstrated exceptional sensitivity, capable of detecting T. gondii-specific antibodies in sera diluted up to 1:8. Specificity evaluation confirmed no cross-reactivity with antibodies against other parasites, such as Neospora caninum, Cryptosporidium suis, Eimeria tenella, and Sarcocystis tenella. Stability tests revealed the test strips maintained full functionality after 12 weeks of storage at 24 \u00b0C. The coincidence rate of the colloidal gold test strips prepared in this study with a commercial ELISA kit was 94.59%. Comparisons with advanced serodiagnostic tools, such as chimeric antigen-based ELISAs and recombinant protein diagnostics, further highlighted its robustness and applicability. These findings underscore the potential of the rMIC2-MIC3-based ICT as a reliable, economical, and accessible diagnostic tool for toxoplasmosis in veterinary and human medicine.<\/jats:p>","DOI":"10.3390\/vetsci12060509","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T04:32:37Z","timestamp":1747888357000},"page":"509","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of an Immunochromatographic Test with Recombinant MIC2-MIC3 Fusion Protein for Serological Detection of Toxoplasma gondii"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-2318","authenticated-orcid":false,"given":"Jianzhong","family":"Wang","sequence":"first","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030810, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2950-230X","authenticated-orcid":false,"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 29, Saihan District, Hohhot 010011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jicheng","family":"Qiu","sequence":"additional","affiliation":[{"name":"Beijing Yuanda Xinghuo Medicine Technology Co., Ltd., Beijing 102615, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030810, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030810, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xialin","family":"Ma","sequence":"additional","affiliation":[{"name":"Hangzhou Evegen Biotech Co., Ltd., Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojie","family":"Wu","sequence":"additional","affiliation":[{"name":"Hangzhou Evegen Biotech Co., Ltd., Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoguang","family":"Li","sequence":"additional","affiliation":[{"name":"Hangzhou Evegen Biotech Co., Ltd., Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Mao","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 29, Saihan District, Hohhot 010011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiduo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Beijing Vocational College of Agriculture, Beijing 102442, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.pt.2019.07.001","article-title":"\u00a0Toxoplasma gondii","volume":"35","author":"Lourido","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_2","first-page":"947","article-title":"Global Status of Toxoplasma gondii Infection: Systematic Review and Prevalence Snapshots","volume":"36","author":"Molan","year":"2019","journal-title":"Trop. 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