{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:18:57Z","timestamp":1760059137603,"version":"build-2065373602"},"reference-count":56,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T00:00:00Z","timestamp":1748044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Bologna, RFO 2024 Funds"},{"name":"European Open Science Cloud (EOSC) Secretariat"},{"name":"EGI call for COVID-19 research projects (AnGen1H project)"},{"name":"POR FESR Emilia-Romagna 2014\u20132020"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Pigs are affected by a variety of pathogenic agents that need to be identified correctly and diagnosed even when co-infections may complicate the application of specific and targeted assays. Next-generation sequencing can provide new perspective to monitor viruses infecting or co-infecting diseased pigs. In this study, we tested, for the first time for diagnostic purposes in a livestock species, a new method based on whole-genome sequencing of all the DNAs extracted from the blood of nine pigs sampled from a farm where there was a suspected outbreak of Post-weaning Multisystemic Wasting Syndrome. We then used unmapped reads on the porcine reference genome to mine for viral sequences using a specifically designed bioinformatic pipeline. Within this fraction of reads, viral sequences ranged from 0.002% to 4.4% of the total unmapped reads and were derived from twelve different viruses known to infect pigs, where three were herpesviruses, eight were parvoviruses, and one was a circovirus. All pig sequencing datasets were positive for one or more viruses, with various potential viral loads. Suid betaherpesvirus 2, also known as Porcine cytomegalovirus (PCMV), was the most frequently identified virus as five out of the nine pig sequencing datasets contained viral sequences from this virus. The results may suggest a heterogeneous viral profile of the diseased pigs that may be derived from potential secondary infections or co-infections. This pilot application demonstrated that a whole-genome sequencing approach can complement other routine diagnostic assays in veterinary virology. Other studies and improvements are needed to validate the results and apply this approach in routine monitoring applications.<\/jats:p>","DOI":"10.3390\/vetsci12060513","type":"journal-article","created":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T20:26:50Z","timestamp":1748204810000},"page":"513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mining Porcine Blood Whole-DNA Sequencing Datasets to Uncover Pig Viromes: An Exploratory Application to Identify Potential Infecting Agents of an Undefined Disease Outbreak"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5712-8211","authenticated-orcid":false,"given":"Samuele","family":"Bovo","sequence":"first","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6778-1938","authenticated-orcid":false,"given":"Anisa","family":"Ribani","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppina","family":"Schiavo","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Taurisano","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4985-0191","authenticated-orcid":false,"given":"Matteo","family":"Bolner","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Bertolini","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7050-3760","authenticated-orcid":false,"given":"Luca","family":"Fontanesi","sequence":"additional","affiliation":[{"name":"Animal and Food Genomics Group, Division of Animal Sciences, Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cassedy, A., Parle-McDermott, A., and O\u2019Kennedy, R. 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