{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:19:12Z","timestamp":1760059152803,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Direcci\u00f3n General de Sostenibilidad, Servicio de Conservaci\u00f3n de la Naturaleza","award":["274\/21"],"award-info":[{"award-number":["274\/21"]}]},{"name":"Servicio de Sanidad Animal","award":["274\/21"],"award-info":[{"award-number":["274\/21"]}]},{"name":"Consejer\u00eda de Agricultura, Ganader\u00eda y Desarrollo Sostenible, Junta de Extremadura","award":["274\/21"],"award-info":[{"award-number":["274\/21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The Iberian lynx (Lynx pardinus) population has been increasing in recent years thanks to the collaboration of several associations within the framework of the Life Lynxconnect project, which promotes captive breeding for the subsequent release of specimens into the environment. It is therefore important to know their population status, the diseases to which these animals are exposed, and how they affect their repopulation. In this sense, this study aims to study how the presence of mycobacteria affects the lynx population in Extremadura (southwest of Spain). To this end, blood samples, tracheal swabs, and tracheobronchial washings from live-captured lynxes, as well as mediastinal and mesenteric lymph nodes from roadkill animals, were collected. The association between the presence of mycobacteria and various factors\u2014including body condition, age, sex, and blood parameters\u2014was subsequently evaluated. Up to date, our findings show the absence of tuberculosis (TB) in all lynxes tested, while they seem to be reservoirs of non-tuberculous mycobacteria (NTM), mainly M. lentiflavum, the most isolated species. Nonetheless, these mycobacteria appear to have no significant effect on the health condition of the animals.<\/jats:p>","DOI":"10.3390\/vetsci12060527","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T03:30:19Z","timestamp":1748489419000},"page":"527","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Non-Tuberculous Mycobacteria in Iberian Lynx (Lynx pardinus) and Their Impact on Its Health"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8051-5738","authenticated-orcid":false,"given":"Natalia","family":"Jim\u00e9nez-Pizarro","sequence":"first","affiliation":[{"name":"Animal Health Department, Faculty of Veterinary, University of Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Serrano","sequence":"additional","affiliation":[{"name":"Life Lynxconnect Project, FOTEX S.L., 06011 Badajoz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-1315","authenticated-orcid":false,"given":"Jorge","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Natural Environment Area, Public Management Society of Extremadura (GPEX), Junta de Extremadura, 06187 M\u00e9rida, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3341-7331","authenticated-orcid":false,"given":"Rafael","family":"Barrera","sequence":"additional","affiliation":[{"name":"MINVET Group, Animal Medicine Department, Faculty of Veterinary, University of Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Gil-Molino","sequence":"additional","affiliation":[{"name":"Animal Health Department, Faculty of Veterinary, University of Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3405-8801","authenticated-orcid":false,"given":"David","family":"Risco","sequence":"additional","affiliation":[{"name":"Animal Medicine Department, Faculty of Veterinary, University of Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5574-4432","authenticated-orcid":false,"given":"Javier","family":"Hermoso-de-Mendoza","sequence":"additional","affiliation":[{"name":"Animal Health Department, Faculty of Veterinary, University of Extremadura, 10003 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1099\/ijsem.0.004332","article-title":"List of prokaryotic names with standing in nomenclature (LPSN) moves to the DSMZ","volume":"70","author":"Parte","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_2","first-page":"e2978","article-title":"Beyond tuberculosis: Diversity and implications of non-tuberculous mycobacteria at the wildlife\u2013livestock interface","volume":"69","author":"Barral","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1111\/j.1939-1676.2012.01019.x","article-title":"Paratuberculosis (Johne\u2019s Disease) in Cattle and Other Susceptible Species","volume":"26","author":"Sweeney","year":"2012","journal-title":"J. Vet. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nishiuchi, Y., Iwamoto, T., and Maruyama, F. (2017). Infection sources of a common non-tuberculous mycobacterial pathogen, Mycobacterium avium complex. Front. Med., 4.","DOI":"10.3389\/fmed.2017.00027"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/CMR.00055-15","article-title":"Methodological and clinical aspects of the molecular epidemiology of Mycobacterium tuberculosis and other mycobacteria","volume":"29","author":"Jagielski","year":"2016","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/CMR.00038-17","article-title":"Practice guidelines for clinical microbiology laboratories: Mycobacteria","volume":"31","author":"Forbes","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bo, M., Arru, G., Niegowska, M., Erre, G.L., Manchia, P.A., and Sechi, L.A. (2019). Association between Lipoprotein Levels and Humoral Reactivity to Mycobacterium avium subsp. paratuberculosis in Multiple Sclerosis, Type 1 Diabetes Mellitus and Rheumatoid Arthritis. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100423"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S69","DOI":"10.1016\/j.rvsc.2014.08.007","article-title":"Non-tuberculous mycobacterial infections of veterinary relevance","volume":"97","author":"Biet","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"ref_9","unstructured":"Tato Jim\u00e9nez, \u00c1. (1999). Infecciones Por Mycobacterium spp. en Animales Salvajes en la Provincia de C\u00e1ceres. [Ph.D. Thesis, Universidad de Extremadura]."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.tvjl.2010.05.003","article-title":"Infection of Eurasian badgers (Meles meles) with Mycobacterium avium complex (MAC) bacteria","volume":"188","author":"Balseiro","year":"2011","journal-title":"Vet. J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz-Mendoza, M., Marreros, N., Boadella, M., Gort\u00e1zar, C., Men\u00e9ndez, S., de Juan, L., Bezos, J., Romero, B., Copano, M.F., and Amado, J. (2013). Wild boar tuberculosis in Iberian Atlantic Spain: A different picture from Mediterranean habitats. BMC Vet. Res., 9.","DOI":"10.1186\/1746-6148-9-176"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/tbed.12083","article-title":"Non-tuberculous Mycobacteria in Wild Boar (Sus scrofa) from Southern Spain: Epidemiological, Clinical and Diagnostic Concerns","volume":"62","author":"Carranza","year":"2015","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gortazar, C., Torres, M.J., Acevedo, P., Aznar, J., Negro, J.J., de la Fuente, J., and Vicente, J. (2011). Fine-tuning the space, time, and host distribution of mycobacteria in wildlife. BMC Microbiol., 11.","DOI":"10.1186\/1471-2180-11-27"},{"key":"ref_14","unstructured":"(2025, May 19). Censo del Lince 2021. Ministerio para la Transici\u00f3n Ecol\u00f3gica y el Reto Demogr\u00e1fico. Available online: https:\/\/www.miteco.gob.es\/content\/dam\/miteco\/es\/biodiversidad\/temas\/inventarios-nacionales\/lince\/censodelinceiberico2021_tcm30-541552.pdf."},{"key":"ref_15","unstructured":"(2025, May 19). Censo del Lince 2022. Ministerio para la Transici\u00f3n Ecol\u00f3gica y el Reto Demogr\u00e1fico. Available online: https:\/\/www.miteco.gob.es\/content\/dam\/miteco\/es\/biodiversidad\/temas\/inventarios-nacionales\/lince\/informecensodelinceiberico2022_tcm30-569643.pdf."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"N\u00e1jera, F., Grande-G\u00f3mez, R., Pe\u00f1a, J., V\u00e1zquez, A., Palacios, M.J., Rueda, C., Corona-Bravo, A.I., Zorrilla, I., Revuelta, L., and Gil-Molino, M. (2021). Disease Surveillance during the Reintroduction of the Iberian Lynx (Lynx pardinus) in Southwestern Spain. Animals, 11.","DOI":"10.3390\/ani11020547"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1177\/104063870802000325","article-title":"Detection of Feline leukemia virus in the endangered Iberian lynx (Lynx pardinus)","volume":"20","author":"Luaces","year":"2008","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gort\u00e1zar, C., Torres, M.J., Vicente, J., Acevedo, P., Reglero, M., de la Fuente, J., Negro, J.J., and Aznar Mart\u00edn, J. (2008). Bovine Tuberculosis in Do\u00f1ana Biosphere Reserve: The Role of Wild Ungulates as Disease Reservoirs in the Last Iberian Lynx Strongholds. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002776"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1099\/ijsem.0.002507","article-title":"Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis","volume":"68","author":"Riojas","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1111\/jam.13090","article-title":"First molecular detection of Mycobacterium bovis in environmental samples from a French region with endemic bovine tuberculosis","volume":"120","author":"Barbier","year":"2016","journal-title":"J. Appl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li\u00e9bana, E., Aranaz, A., Francis, B., and Cousins, D. (1996). Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex. J. Clin. Microbiol., 34.","DOI":"10.1128\/jcm.34.4.933-938.1996"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0378-1135(91)90010-D","article-title":"Use of DNA amplification for the rapid identification of Mycobacterium bovis","volume":"27","author":"Cousins","year":"1991","journal-title":"Vet. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1128\/jcm.31.2.175-178.1993","article-title":"Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis","volume":"31","author":"Telenti","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Chimara, E., Ferrazoli, L., Ueky, S.Y.M., Martins, M.C., Durham, A.M., Arbeit, R.D., and Le\u00e3o, S.C. (2008). Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65 patterns. BMC Microbiol., 8.","DOI":"10.1186\/1471-2180-8-48"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF03192659","article-title":"Feeding ecology of the Iberian lynx Lynx pardinus in eastern Sierra Morena (Southern Spain)","volume":"51","year":"2006","journal-title":"Acta Theriol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1128\/CMR.16.2.319-354.2003","article-title":"Impact of genotypic studies on mycobacterial taxonomy: The new mycobacteria of the 1990s","volume":"16","author":"Tortoli","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"395","DOI":"10.3201\/eid1703.090948","article-title":"Mycobacterium lentiflavum in Drinking Water Supplies, Australia","volume":"17","author":"Marshall","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/j.fm.2009.04.005","article-title":"Detection of nontuberculous mycobacteria from water buffalo raw milk in Brazil","volume":"26","author":"Lopes","year":"2009","journal-title":"Food Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"97","DOI":"10.18683\/germs.2021.1245","article-title":"An update on prevalence of slow-growing mycobacteria and rapid-growing mycobacteria retrieved from hospital water sources in Iran\u2014A systematic review","volume":"11","author":"Arfaatabar","year":"2021","journal-title":"Germs"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Katale, B.Z., Mbugi, E.V., Botha, L., Keyyu, J.D., Kendall, S., Dockrell, H.M., Michel, A.L., Kazwala, R.R., Rweyemamu, M.M., and van Helden, P. (2014). Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania. BMC Infect. Dis., 14.","DOI":"10.1186\/s12879-014-0616-y"},{"key":"ref_31","first-page":"e329","article-title":"Mycobacterium lentiflavum lymphadenitis: Two case reports","volume":"114","author":"Cirac","year":"2016","journal-title":"Arch. Argent. Pediatr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1183\/13993003.00535-2020","article-title":"Treatment of Nontuberculous Mycobacterial Pulmonary Disease: AnOfficial ATS\/ERS\/ESCMID\/IDSA Clinical Practice Guideline","volume":"56","author":"Daley","year":"2020","journal-title":"Eur. Respir. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.5858\/2008-132-1333-NMI","article-title":"Nontuberculous Mycobacterial Infections","volume":"132","author":"Jarzembowski","year":"2008","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.eimc.2017.12.006","article-title":"Mycobacterium lentiflavum como causa principal de linfadenitis en poblaci\u00f3n pedi\u00e1trica","volume":"36","year":"2018","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"315","DOI":"10.4103\/ijmy.ijmy_58_17","article-title":"Recovery of Mycobacterium lentiflavum from bronchial lavage during follow-up of an extrapulmonary tuberculosis patient","volume":"6","author":"Vise","year":"2017","journal-title":"Int. J. Mycobacteriol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Piersimoni, C., Goteri, G., Nista, D., Mariottini, A., Mazzarelli, G., and Bornigia, S. (2004). Mycobacterium lentiflavum as an Emerging Causative Agent of Cervical Lymphadenitis. J. Clin. Microbiol., 42.","DOI":"10.1128\/JCM.42.8.3894-3897.2004"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ijid.2017.12.001","article-title":"Clinical characteristics of pulmonary Mycobacterium lentiflavum disease in adult patients","volume":"67","author":"Yagi","year":"2018","journal-title":"Int. J. Infect. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1672\/07-18.1","article-title":"Food habits of wild boars (Sus scrofa) in a Mediterranean coastal wetland","volume":"28","author":"Herrero","year":"2008","journal-title":"Wetlands"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s10164-004-0121-7","article-title":"The sexual function of wallowing in male wild boar (Sus scrofa)","volume":"23","year":"2005","journal-title":"J. Ethol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"495074","DOI":"10.4061\/2011\/495074","article-title":"Mycobacteria in Terrestrial Small Mammals on Cattle Farms in Tanzania","volume":"2011","author":"Durnez","year":"2011","journal-title":"Vet. Med. Int."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Varela-Castro, L., Torrontegi, O., Sevilla, I.A., and Barral, M. (2020). Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030374"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/6\/527\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:42:45Z","timestamp":1760031765000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/6\/527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,29]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["vetsci12060527"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12060527","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2025,5,29]]}}}