{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:22:02Z","timestamp":1792135322685,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Autonomous Region Animal Disease Purification Technology Innovation Team Project","award":["2020CXTDLX05"],"award-info":[{"award-number":["2020CXTDLX05"]}]},{"name":"Autonomous Region Animal Disease Purification Technology Innovation Team Project","award":["2024BBF02021"],"award-info":[{"award-number":["2024BBF02021"]}]},{"name":"Autonomous Region Animal Disease Purification Technology Innovation Team Project","award":["2024FRD05072"],"award-info":[{"award-number":["2024FRD05072"]}]},{"name":"Autonomous Region Animal Disease Purification Technology Innovation Team Project","award":["2022AAC03712"],"award-info":[{"award-number":["2022AAC03712"]}]},{"name":"Autonomous Region Animal Disease Purification Technology Innovation Team Project","award":["2023AAC02075"],"award-info":[{"award-number":["2023AAC02075"]}]},{"name":"Key Research and Development Project for Characteristic Industries in Ningxia Hui Autonomous Region in 2024","award":["2020CXTDLX05"],"award-info":[{"award-number":["2020CXTDLX05"]}]},{"name":"Key Research and Development Project for Characteristic Industries in Ningxia Hui Autonomous Region in 2024","award":["2024BBF02021"],"award-info":[{"award-number":["2024BBF02021"]}]},{"name":"Key Research and Development Project for Characteristic Industries in Ningxia Hui Autonomous Region in 2024","award":["2024FRD05072"],"award-info":[{"award-number":["2024FRD05072"]}]},{"name":"Key Research and Development Project for Characteristic Industries in Ningxia Hui Autonomous Region in 2024","award":["2022AAC03712"],"award-info":[{"award-number":["2022AAC03712"]}]},{"name":"Key Research and Development Project for Characteristic Industries in Ningxia Hui Autonomous Region in 2024","award":["2023AAC02075"],"award-info":[{"award-number":["2023AAC02075"]}]},{"name":"Local Science and Technology Development Projects","award":["2020CXTDLX05"],"award-info":[{"award-number":["2020CXTDLX05"]}]},{"name":"Local Science and Technology Development Projects","award":["2024BBF02021"],"award-info":[{"award-number":["2024BBF02021"]}]},{"name":"Local Science and Technology Development Projects","award":["2024FRD05072"],"award-info":[{"award-number":["2024FRD05072"]}]},{"name":"Local Science and Technology Development Projects","award":["2022AAC03712"],"award-info":[{"award-number":["2022AAC03712"]}]},{"name":"Local Science and Technology Development Projects","award":["2023AAC02075"],"award-info":[{"award-number":["2023AAC02075"]}]},{"name":"Natural Science Foundation of Ningxia Province","award":["2020CXTDLX05"],"award-info":[{"award-number":["2020CXTDLX05"]}]},{"name":"Natural Science Foundation of Ningxia Province","award":["2024BBF02021"],"award-info":[{"award-number":["2024BBF02021"]}]},{"name":"Natural Science Foundation of Ningxia Province","award":["2024FRD05072"],"award-info":[{"award-number":["2024FRD05072"]}]},{"name":"Natural Science Foundation of Ningxia Province","award":["2022AAC03712"],"award-info":[{"award-number":["2022AAC03712"]}]},{"name":"Natural Science Foundation of Ningxia Province","award":["2023AAC02075"],"award-info":[{"award-number":["2023AAC02075"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Brucellosis is caused by Brucella spp.; it can result in fetal loss and abortion, resulting in economic losses and negative effects on human health. Herein, a cross-sectional study on the epidemiology of Brucella spp. in aborted livestock in Ningxia from 2022 to 2023 was conducted. A total of 749 aborted tissue samples from 215 cattle and 534 sheep were collected from farmers who reported abortions that were supported by veterinarians trained in biosecurity. The samples were analyzed using qPCR and were cultured for Brucella spp. when a positive result was obtained; the samples were speciated using AMOS-PCR. MLST and MLVA were employed for genotype identification. The results demonstrated that 8.68% of the samples were identified as being positive for Brucella spp. based on qPCR results. In total, 14 field strains of Brucella spp. were subsequently isolated, resulting in 11 B. melitensis, 2 B. abortus, and 1 B. suis. being identified via AMOS-PCR. Four sequence types were identified via MLST\u2014ST7 and ST8 (B. melitensis), ST2 (B. abortus), and ST14 (B. suis)\u2014with ST8 predominating. Five MLVA-8 genotypes and seven MLVA-11 genotypes were identified, with MLVA-11 GT116 predominating in livestock. Thus, at least three Brucella species are circulating in aborted livestock in Ningxia. This suggests a significant risk of transmission to other animals and humans. Therefore, disinfection and safe treatment procedures for aborted livestock and their products should be carried out to interrupt the transmission pathway; aborted livestock should be examined to determine zoonotic causes and targeted surveillance should be strengthened to improve the early detection of infectious causes, which will be of benefit to the breeding industry and public health security.<\/jats:p>","DOI":"10.3390\/vetsci12080702","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T10:51:14Z","timestamp":1753699874000},"page":"702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Epidemiology of Brucella spp. in Aborted Livestock in the Ningxia Hui Autonomous Region, China"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8533-438X","authenticated-orcid":false,"given":"Cai","family":"Yin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Yang","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Wu","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Di","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taotao","family":"Bai","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longlong","family":"Luo","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Ma","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"},{"name":"School of Agriculture, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoying","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixin","family":"Li","sequence":"additional","affiliation":[{"name":"Ningxia Hui Autonomous Region Center for Animal Disease Control and Prevention, Yinchuan 750011, China"},{"name":"School of Agriculture, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1002\/vms3.805","article-title":"Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda","volume":"8","author":"Ntivuguruzwa","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/avj.13040","article-title":"Ovine abortion and stillbirth investigations in Australia","volume":"99","author":"Clune","year":"2020","journal-title":"Aust. Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1051\/vetres:2005003","article-title":"From the discovery of the Malta fevers agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis","volume":"36","author":"Godfroid","year":"2005","journal-title":"Vet. Res."},{"key":"ref_4","first-page":"27","article-title":"Public Health and Economic Importance of Bovine Brucellosis: An Overview","volume":"5","author":"Pal","year":"2017","journal-title":"Am. J. Epidemiol. Infect. Dis."},{"key":"ref_5","first-page":"25","article-title":"Brucellosis in Cattle and Buffaloes","volume":"2","author":"Mustafa","year":"2023","journal-title":"Int. J. Agric. Biol."},{"key":"ref_6","unstructured":"Spickler, A.R. (2025, July 22). Brucellosis. Available online: http:\/\/www.cfsph.iastate.edu\/DiseaseInfo\/factsheets.php."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Fereig, R.M., Wareth, G., Abdelbaky, H.H., Mazeed, A.M., El-Diasty, M., Abdelkhalek, A., Mahmoud, H.Y.A.H., Ali, A.O., El-tayeb, A., and Alsayeqh, A.F. (2022). Seroprevalence of Specific Antibodies to Toxoplasma gondii, Neospora caninum, and Brucella spp. in Sheep and Goats in Egypt. Animals, 12.","DOI":"10.3390\/ani12233327"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ma, H.-R., Xu, H.-J., Wang, X., Bu, Z.-Y., Yao, T., Zheng, Z.-R., Sun, Y., Ji, X., and Liu, J. (2023). Molecular characterization and antimicrobial susceptibility of human Brucella in Northeast China. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1137932"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.14202\/vetworld.2024.1778-1788","article-title":"Molecular typing methods to characterize Brucella spp. from animals: A review","volume":"17","author":"Daugaliyeva","year":"2024","journal-title":"Vet. World"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Moreno, E. (2014). Retrospective and prospective perspectives on zoonotic brucellosis. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00213"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e135","DOI":"10.1086\/523726","article-title":"The Many Faces of Human-to-Human Transmission of Brucellosis: Congenital Infection and Outbreak of Nosocomial Disease Related to an Unrecognized Clinical Case","volume":"45","author":"Mesner","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e00073-19","DOI":"10.1128\/CMR.00073-19","article-title":"Laboratory Diagnosis of Human Brucellosis","volume":"33","author":"Yagupsky","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tan, Q., Wang, Y., Liu, Y., Tao, Z., Yu, C., Huang, Y., Yang, X., Ying, X., Hu, Y., and Li, S. (2023). Molecular epidemiological characteristics of Brucella in Guizhou Province, China, from 2009 to 2021. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1188469"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Piao, D.-R., Liu, X., Di, D.-D., Xiao, P., Zhao, Z.-Z., Xu, L.-Q., Tian, G.-Z., Zhao, H.-Y., Fan, W.-X., and Cui, B.-Y. (2018). Genetic polymorphisms identify in species\/biovars of Brucella isolated in China between 1953 and 2013 by MLST. BMC Microbiol., 18.","DOI":"10.1186\/s12866-018-1149-0"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Liu, G., Ma, X., Zhang, R., L\u00fc, J., Zhou, P., Liu, B., Liu, T., Ren, H., Liu, Z., and Li, Z. (2024). Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1320845"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s11250-013-0448-6","article-title":"Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR","volume":"46","author":"Ali","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"105635","DOI":"10.1016\/j.meegid.2024.105635","article-title":"Molecular epidemiology of Brucella abortus isolated from the environment in Ningxia Hui autonomous region, China","volume":"123","author":"Yang","year":"2024","journal-title":"Infect. Genet. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/jcm.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella-ovis, and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Whatmore, A.M., Koylass, M.S., Muchowski, J., Edwards-Smallbone, J., Gopaul, K.K., and Perrett, L.L. (2016). Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.02049"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/bioinformatics\/btw582","article-title":"PHYLOViZ 2.0: Providing scalable data integration and visualization for multiple phylogenetic inference methods","volume":"33","author":"Nascimento","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.12.015","article-title":"Evaluation of Brucella MLVA typing for human brucellosis","volume":"69","author":"Jacques","year":"2007","journal-title":"J. Microbiol. Meth."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Jiang, H., Wang, H., Xu, L., Hu, G., Ma, J., Xiao, P., Fan, W., Di, D., Tian, G., and Fan, M. (2013). MLVA Genotyping of Brucella melitensis and Brucella abortus Isolates from Different Animal Species and Humans and Identification of Brucella suis Vaccine Strain S2 from Cattle in China. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0076332"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.3168\/jds.2023-23823","article-title":"Enhancing bovine abortion surveillance: A learning experience","volume":"107","author":"Ribbens","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Clegg, S., Zhang, H., Deng, X., Cui, B., Shao, Z., Zhao, X., Yang, Q., Song, S., Wang, Z., and Wang, Y. (2020). Abortion and various associated risk factors in dairy cow and sheep in Ili, China. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232568"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.prevetmed.2015.03.013","article-title":"Economic losses occurring due to brucellosis in Indian livestock populations","volume":"119","author":"Singh","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1080\/01652176.2021.1894501","article-title":"Importance of brucellosis control programs of livestock on the improvement of one health","volume":"41","author":"Dadar","year":"2021","journal-title":"Vet. Q."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Gong, W., Sun, P., Zhai, C., Yuan, J., Chen, Y., Chen, Q., and Zhao, Y. (2023). Accessibility of the three-year comprehensive prevention and control of brucellosis in Ningxia: A mathematical modeling study. BMC Infect. Dis., 23.","DOI":"10.1186\/s12879-023-08270-4"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Heidary, M., Dashtbin, S., Ghanavati, R., Mahdizade Ari, M., Bostanghadiri, N., Darbandi, A., Navidifar, T., and Talebi, M. (2022). Evaluation of Brucellosis Vaccines: A Comprehensive Review. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.925773"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s40249-022-01044-1","article-title":"First record of the human infection of Brucella melitensis in Kyrgyzstan: Evidence from whole-genome sequencing-based analysis","volume":"11","author":"Kydyshov","year":"2022","journal-title":"Infect. Dis. Poverty"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1080\/22221751.2020.1720528","article-title":"Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016","volume":"9","author":"Liu","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40249-016-0123-z","article-title":"MLVA and MLST typing of Brucella from Qinghai, China","volume":"5","author":"Ma","year":"2016","journal-title":"Infect. Dis. Poverty"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Liu, Z.-g., Wang, M., Zhao, H.-y., Piao, D.-r., Jiang, H., and Li, Z.-j. (2019). Investigation of the molecular characteristics of Brucella isolates from Guangxi Province, China. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1665-6"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bronner, A., H\u00e9naux, V., Fortan\u00e9, N., Hendrikx, P., and Calavas, D. (2014). Why do farmers and veterinarians not report all bovine abortions, as requested by the clinical brucellosis surveillance system in France. BMC Vet. Res., 10.","DOI":"10.1186\/1746-6148-10-93"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"435","DOI":"10.20506\/rst.29.3.1988","article-title":"To report or not to report: A psychosocial investigation aimed at improving early detection of avian influenza outbreaks","volume":"29","author":"Elbers","year":"2010","journal-title":"Rev. Sci. Tech."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.4102\/ojvr.v90i1.2010","article-title":"Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants","volume":"90","author":"Jonker","year":"2023","journal-title":"Onderstepoort J. Vet. Res."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/8\/702\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:17:12Z","timestamp":1760033832000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/8\/702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["vetsci12080702"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12080702","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,28]]}}}