{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:23:57Z","timestamp":1780417437682,"version":"3.54.1"},"reference-count":31,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Lacrimal cysts (dacryops), which involve lacrimal tissue, are uncommon in dogs with an obscure\/unclear pathogenesis. Compared to the current available literature, this report describes the clinicopathologic and immunohistochemical features of two cases of unusual dacryops in brachycephalic dogs. A three-year-old male Cane Corso was referred with a 1-month history of swelling ventromedial to the left eye associated with blepharospasm and epiphora. Furthermore, a severe lower and upper eyelid entropion and a deep corneal ulcer were present. B-mode ultrasonography and a CT scan revealed a subcutaneous cyst, closely adherent to the maxillary bone. Surgical removal and the correction of entropion were performed. No recurrence and\/or complication was detected by seven-year follow-up. Histopathology revealed a cystic structure with single- to double-cell-layered, nonciliated, cuboidal epithelia. Alcian blue stain revealed rare, disseminated goblet cells admixed with epithelial cells. The epithelium was strongly Cytokeratin-positive by immunohistochemistry and appeared lined by several layers of smooth muscle actin (SMA)-positive myoepithelial cells. A 1-year-old male French Bulldog with a 3-month lesion of the third eyelid of the right eye. The lesion (15 mm \u00d7 7 mm) beneath the conjunctiva appeared pale-pink, smooth, and multilobulated. Excision was performed by blunt dissection through the conjunctiva on the palpebral surface of the third eyelid. Recovery was uncomplicated, and no recurrence has been noted at three-year follow-up. Cytology of the cystic fluid and histopathology and immunohistochemistry of the cyst wall revealed findings for case 1. To further characterize the SMA-positive spindle cells located directly beneath the cyst-lining epithelium, double-color immunofluorescence for SMA and p63 (a myoepithelial cell marker) was performed on the sample from case 2. The analysis revealed that the SMA-positive cells lacked p63 expression, indicating a non-myoepithelial phenotype. The histological findings in our cases are consistent with previous reports of canine dacryops. The positivity of immunohistochemical staining for SMA in cells directly beneath the epithelium of dacryops in the cases here described in two brachycephalic dogs is consistent with previous reports in dogs and horses but in contrast with a retrospective study about a human dacryops. These results support the conclusion that the pathogenesis of dacryops in dogs should exclude failure of ductular \u201cneuromuscular\u201d contractility.<\/jats:p>","DOI":"10.3390\/vetsci12080705","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T14:05:47Z","timestamp":1753711547000},"page":"705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinicopathologic Features, Surgical Treatment, and Pathological Characterization of Canine Dacryops with Different Localization"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5865-3774","authenticated-orcid":false,"given":"Barbara","family":"Lamagna","sequence":"first","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-5016","authenticated-orcid":false,"given":"Luigi","family":"Navas","sequence":"additional","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0399-1239","authenticated-orcid":false,"given":"Francesco","family":"Prisco","sequence":"additional","affiliation":[{"name":"Unit of Pathology, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7310-0246","authenticated-orcid":false,"given":"Dario","family":"Costanza","sequence":"additional","affiliation":[{"name":"Interdepartmental Center of Veterinary Radiology, University of Napoli \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Russo","sequence":"additional","affiliation":[{"name":"Unit of Pathology, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Lamagna","sequence":"additional","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Di Palma","sequence":"additional","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Uccello","sequence":"additional","affiliation":[{"name":"Veterinary Center ASL Napoli 1, 80145 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Mennonna","sequence":"additional","affiliation":[{"name":"Veterinary Center ASL Napoli 1, 80145 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3091-0905","authenticated-orcid":false,"given":"Orlando","family":"Paciello","sequence":"additional","affiliation":[{"name":"Unit of Pathology, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flaviana La","family":"Peruta","sequence":"additional","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Flauto","sequence":"additional","affiliation":[{"name":"ASL Napoli 2 Nord, 80027 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5424-6335","authenticated-orcid":false,"given":"Giovanni","family":"Della Valle","sequence":"additional","affiliation":[{"name":"Unit of Surgery, Department of Veterinary Medicine and Animal Productions, University of Naples \u201cFederico II\u201d, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/vop.12118","article-title":"Acquired Canaliculocele with Intranasal Extension in a Dog","volume":"18","author":"Durieux","year":"2015","journal-title":"Vet. Ophthalmol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"736","DOI":"10.2460\/javma.1977.171.08.736","article-title":"Lacrimal Cyst (Dacryops) in 2 Dogs","volume":"171","author":"Playter","year":"1977","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.2460\/javma.1983.183.09.1003","article-title":"Membrana Nictitans Gland Cyst in a Dog","volume":"183","author":"Latimer","year":"1983","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_4","first-page":"1432","article-title":"Orbital Cyst with Conjunctival Fistula in a Dog","volume":"153","author":"Harvey","year":"1968","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.5326\/15473317-31-1-15","article-title":"Epiphora Associated with Dacryops in a Dog","volume":"31","author":"Grahn","year":"1995","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_6","first-page":"935","article-title":"Diagnostic Ophthalmology. Congenital Medial Canthal Cyst","volume":"44","author":"Cullen","year":"2003","journal-title":"Can. Vet. J. Rev. Vet. Can."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"191","DOI":"10.5326\/0450191","article-title":"Dacryops (Lacrimal Cyst) in Three Young Labrador Retrievers","volume":"45","author":"Ota","year":"2009","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1463-5224.2011.00959.x","article-title":"Conjunctival Dacryops in Two Golden Retrievers","volume":"15","author":"Lamagna","year":"2012","journal-title":"Vet. Ophthalmol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1463-5224.2012.01036.x","article-title":"Dacryops of the Lacrimal Gland in a Dog","volume":"16","author":"Delgado","year":"2013","journal-title":"Vet. Ophthalmol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/vop.12860","article-title":"Dacryops with Extensive Lacrimal and Maxillary Bone Defects in Four Dogs","volume":"24","author":"Steinmetz","year":"2021","journal-title":"Vet. Ophthalmol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1002\/vms3.853","article-title":"Dacryops with Dacryolithiasis in a Dog","volume":"8","author":"Susanti","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_12","first-page":"424","article-title":"Epiphora Associated with Canaliculops in a Dog","volume":"27","author":"Gerding","year":"1991","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1463-5224.2012.01065.x","article-title":"Canine Dacryolithiasis: A Case Description and Mineral Analysis","volume":"16","author":"Malho","year":"2013","journal-title":"Vet. Ophthalmol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1463-5224.2011.00930.x","article-title":"Orbito-nasal Cyst in a Young European Short-haired Cat","volume":"14","author":"Matheis","year":"2011","journal-title":"Vet. Ophthalmol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/vop.12394","article-title":"Dacryops of the Third Eyelid in a Cat","volume":"19","author":"Sritrakoon","year":"2016","journal-title":"Vet. Ophthalmol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1111\/vop.12831","article-title":"Bilateral Temporal Dacryops in a Cat","volume":"23","author":"Maggio","year":"2020","journal-title":"Vet. Ophthalmol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/vop.12309","article-title":"Differential Diagnoses, Investigation, and Management of a Periocular Swelling Close to the Nasolacrimal Duct in a Horse\u2013A Case Report of Dacryops","volume":"19","author":"Dawson","year":"2016","journal-title":"Vet. Ophthalmol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1046\/j.1463-5224.2002.00249.x","article-title":"Dacryops in a Red-eared Slider (Chrysemys Scripta Elegans): Case Report","volume":"5","author":"Allgoewer","year":"2002","journal-title":"Vet. Ophthalmol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1159\/000066185","article-title":"Simple Dacryops: A Case Series and Review of the Literature","volume":"216","author":"Betharia","year":"2002","journal-title":"Ophthalmologica"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e108","DOI":"10.1097\/IOP.0000000000000120","article-title":"Canaliculops Associated with Punctal Agenesis: A Clinicopathological Correlation and Review of Literature","volume":"31","author":"Ali","year":"2015","journal-title":"Ophthalmic Plast. Reconstr. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.ajo.2013.06.008","article-title":"Canaliculops: Clinicopathologic Features and Treatment with Marsupialization","volume":"156","author":"Yoon","year":"2013","journal-title":"Am. J. Ophthalmol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1159\/000381646","article-title":"Canalicular Cyst","volume":"1","author":"Jakobiec","year":"2015","journal-title":"Ocul. Oncol. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.ajo.2012.07.028","article-title":"The Cytologic Composition of Dacryops: An Immunohistochemical Investigation of 15 Lesions Compared to the Normal Lacrimal Gland","volume":"155","author":"Jakobiec","year":"2013","journal-title":"Am. J. Ophthalmol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1001\/jamaophthalmol.2013.1885","article-title":"Dacryops: A Series of 5 Cases and a Proposed Pathogenesis","volume":"131","author":"Lam","year":"2013","journal-title":"JAMA Ophthalmol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101150","DOI":"10.1016\/j.psj.2021.101150","article-title":"Pathologic Characterization of White Striping Myopathy in Broiler Chickens","volume":"100","author":"Prisco","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/j.1463-5224.2010.00861.x","article-title":"Computed Tomographic-dacryocystography (CT-DCG) of the Normal Canine Nasolacrimal Drainage System with Three-dimensional Reconstruction","volume":"14","author":"Rached","year":"2011","journal-title":"Vet. Ophthalmol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1740-8261.2004.04004.x","article-title":"Computed Tomography Dacryocystography Evaluation of the Nasolacrimal Apparatus","volume":"45","author":"Nykamp","year":"2004","journal-title":"Vet. Radiol. Ultrasound"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/vop.12644","article-title":"Use of 1% Polidocanol and Pre-and Post-computed Tomography for Treatment of a Suspected Nasolacrimal Duct Cyst in a Dog","volume":"22","author":"Zimmerman","year":"2019","journal-title":"Vet. Ophthalmol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/vop.12110","article-title":"Dacryocystography in a Cat with Orbital Pneumatosis","volume":"18","author":"Meomartino","year":"2015","journal-title":"Vet. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"216","DOI":"10.5326\/0400216","article-title":"Surgical Treatment of Recurrent Dacryocystitis Secondary to Cystic Dilatation of the Nasolacrimal Duct in a Dog","volume":"40","author":"Lussier","year":"2004","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/vop.12382","article-title":"Marsupialization of a Cyst of the Nictitating Membrane in Three Dogs","volume":"20","author":"Mias","year":"2017","journal-title":"Vet. Ophthalmol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/8\/705\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:17:23Z","timestamp":1760033843000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/8\/705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":31,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["vetsci12080705"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12080705","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,28]]}}}