{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:35:03Z","timestamp":1760060103318,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of the Jiangsu Higher Education Institutions of China","award":["No. 22KJB230006","Nos. 2022kj31"],"award-info":[{"award-number":["No. 22KJB230006","Nos. 2022kj31"]}]},{"name":"Natural Science Fund Project of Jiangsu Vocational College of Agriculture and Forestry","award":["No. 22KJB230006","Nos. 2022kj31"],"award-info":[{"award-number":["No. 22KJB230006","Nos. 2022kj31"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The genotoxin colibactin, a complex secondary metabolite, targets eukaryotic cell cycle machinery and contributes to neonatal sepsis and meningitis. Avian pathogenic Escherichia coli (APEC) XM, which produces this genotoxin, is an agent of poultry diseases with zoonotic potential. In this study, we confirmed that clbF was necessary for the APEC XM strain to produce colibactin, but it did not affect the growth, adhesion, or invasion of cells. Deletion of clbF substantially diminished both virulence and systemic dissemination, but it also changed the gene expression of the antiserum survival factor, adherence and invasion, iron acquisition genes, and the secretion system. In conclusion, clbF is necessary for the synthesis of the genotoxin colibactin and affects the development of APEC meningitis in mice.<\/jats:p>","DOI":"10.3390\/vetsci12080727","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T15:56:16Z","timestamp":1754409376000},"page":"727","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of clbF in the Pathogenicity of Avian Pathogenic Escherichia coli"],"prefix":"10.3390","volume":"12","author":[{"given":"Meng","family":"Wu","sequence":"first","affiliation":[{"name":"School of Animal Pharmaceutical, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haitao","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pet Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pet Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Hao","sequence":"additional","affiliation":[{"name":"School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peili","family":"Wang","sequence":"additional","affiliation":[{"name":"The Third Clinical Medical College, Nanjing University of Chinese Medicine, 138 Xianlin Rd, Nanjing 210023, China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1089\/fpd.2013.1533","article-title":"Human and avian extraintestinal pathogenic Escherichia coli: Infections, zoonotic risks, and antibiotic resistance trends","volume":"10","author":"Mellata","year":"2013","journal-title":"Foodborne Pathog. 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