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While NADPH oxidase (NOX) is a critical source of ROS generation, its role in bovine endometrial epithelial cells (BEEC) and modulation by selenium remains unexplored. In this study, primary BEEC was challenged by LPS to assess NOX2\/4 expression kinetics. Inhibitors of NOX and NF-\u03baB were applied to observe the role of NOX-derived ROS in BEEC inflammation and in selenomethionine (SeMet)-modulated anti-inflammation. ROS levels were measured by flow cytometry. The changes in inflammatory cytokines, and the proteins related to NOX4 and NF-\u03baB, were analyzed via qPCR and Western blot. As a result, the inhibition of NOX decreased LPS-induced proinflammatory cytokine expression, ROS accumulation, NOX4 level, and the phosphorylation of NF-\u03baB P65 and I\u03baB\u03b1. Conversely, the suppression of NF-\u03baB downregulated the levels of ROS and NOX4. Cotreatment with SeMet and a NOX inhibitor further suppressed the inflammatory response, ROS level, and NF-\u03baB pathway activation compared to individual treatment, but had no additive effect on the NOX4 protein level. In conclusion, the NOX4\/ROS\/NF-\u03baB axis forms a proinflammatory feedback loop in LPS-stimulated BEEC. SeMet mitigates oxidative stress and inflammation partially through NOX4 inhibition.<\/jats:p>","DOI":"10.3390\/vetsci12090789","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T12:31:26Z","timestamp":1755865886000},"page":"789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inhibition of NOX4-Mediated ROS Production Contributes to Selenomethionine\u2019s Anti-Inflammatory Effect in LPS-Stimulated Bovine Endometrial Epithelial Cells"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9606-5105","authenticated-orcid":false,"given":"Luying","family":"Cui","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasa","family":"He","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agriproduct Safety of the Ministry of Education, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, 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the Ministry of Education, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsheng","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agriproduct Safety of the Ministry of Education, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianji","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agriproduct Safety of the Ministry of Education, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1111\/rda.13815","article-title":"Alterations in biochemical profiles and reproduction performance in postpartum dairy cows with metritis","volume":"55","author":"Paiano","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1093\/humupd\/dmad013","article-title":"Endometrial responses to bacterial and viral infection: A scoping review","volume":"29","author":"Lindsay","year":"2023","journal-title":"Hum. Reprod. Update"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"112361","DOI":"10.1016\/j.jep.2019.112361","article-title":"Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4\/MyD88\/NF-\u03baB signaling pathway","volume":"248","author":"Hu","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.intimp.2018.01.021","article-title":"Cortisol inhibits NF-\u03baB and MAPK pathways in LPS activated bovine endometrial epithelial cells","volume":"56","author":"Dong","year":"2018","journal-title":"Int. Immunopharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"skae260","DOI":"10.1093\/jas\/skae260","article-title":"Selenium suppressed the LPS-induced oxidative stress of bovine endometrial stromal cells through Nrf2 pathway with high cortisol background","volume":"102","author":"Cui","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/s41423-022-00858-1","article-title":"NADPH oxidase family proteins: Signaling dynamics to disease management","volume":"19","author":"Begum","year":"2022","journal-title":"Cell. Mol. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Boni, R., and Gualandi, S.C. (2022). Relationship between oxidative stress and endometritis: Exploiting knowledge gained in mares and cows. Animals, 12.","DOI":"10.3390\/ani12182403"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1021\/acs.chemrestox.9b00524","article-title":"Protective effects of lixisenatide against lipopolysaccharide-induced inflammation response in MAC-T bovine mammary epithelial cells: A therapeutic implication in mastitis","volume":"33","author":"Li","year":"2020","journal-title":"Chem. Res. Toxicol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5042048","DOI":"10.1155\/2021\/5042048","article-title":"Therapeutic role of miR-30a in lipoteichoic acid-induced endometritis via targeting the MyD88\/Nox2\/ROS signaling","volume":"2021","author":"Jiang","year":"2021","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.2147\/JIR.S314130","article-title":"Fisetin ameliorates the inflammation and oxidative stress in lipopolysaccharide-induced endometritis","volume":"14","author":"Jiang","year":"2021","journal-title":"J. Inflamm. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cr.2010.178","article-title":"Crosstalk of reactive oxygen species and NF-\u03baB signaling","volume":"21","author":"Morgan","year":"2011","journal-title":"Cell Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"156471","DOI":"10.1016\/j.cyto.2023.156471","article-title":"The inhibition effect of caffeic acid on NOX\/ROS-dependent macrophages M1-like polarization contributes to relieve the LPS-induced mice mastitis","volume":"174","author":"Yu","year":"2024","journal-title":"Cytokine"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xiao, J., Khan, M.Z., Ma, Y., Alugongo, G.M., Ma, J., Chen, T., Khan, A., and Cao, Z. (2021). The antioxidant properties of selenium and Vitamin E; their role in periparturient dairy cattle health regulation. Antioxidants, 10.","DOI":"10.3390\/antiox10101555"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1007\/s12011-018-1323-0","article-title":"Effect of organic selenium supplementation on selenium status, oxidative stress, and antioxidant status in selenium-adequate dairy cows during the periparturient period","volume":"186","author":"Gong","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.1007\/s12011-024-04457-1","article-title":"Effects of supranutritional selenium supplementation during different trimesters of pregnancy on humoral Iimmunity in beef cattle at parturition","volume":"203","author":"Hall","year":"2024","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"106686","DOI":"10.1016\/j.anireprosci.2021.106686","article-title":"Effects of injectable trace element and vitamin supplementation during the gestational, peri-parturient, or early lactational periods on neutrophil functions and pregnancy rate in dairy cows","volume":"225","author":"Pekcan","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1039\/C9FO02415H","article-title":"Selenium alleviates lipopolysaccharide-induced endometritis via regulating the recruitment of TLR4 into lipid rafts in mice","volume":"11","author":"Chen","year":"2020","journal-title":"Food Funct."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cui, L., Zhong, J., Duan, J., Li, W., Mao, P., Dong, J., Liu, K., Guo, L., Wang, H., and Li, J. (2025). The antioxidant effect of selenium is enhanced by cortisol through Nrf2 pathway in bovine endometrial epithelial cells. Animals, 15.","DOI":"10.3390\/ani15081075"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cui, L., Zhang, M., Zheng, F., Yuan, C., Wang, Z., Qiu, S., Meng, X., Dong, J., Liu, K., and Guo, L. (2024). Selenium elicited an enhanced anti-inflammatory effect in primary bovine endometrial stromal cells with high cortisol background. BMC Vet. Res., 20.","DOI":"10.1186\/s12917-024-04240-3"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112101","DOI":"10.1016\/j.yexcr.2020.112101","article-title":"A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries","volume":"394","author":"Lv","year":"2020","journal-title":"Exp. Cell Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.3892\/mmr.2014.2239","article-title":"Resveratrol inhibits oxygen-glucose deprivation-induced MMP-3 expression and cell apoptosis in primary cortical cells via the NF-\u03baB pathway","volume":"10","author":"Huang","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.lfs.2015.04.024","article-title":"Suppression of methylmercury-induced IL-6 and MCP-1 expressions by N-acetylcysteine in U-87MG human astrocytoma cells","volume":"134","author":"Muniroh","year":"2015","journal-title":"Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"108989","DOI":"10.1016\/j.intimp.2022.108989","article-title":"Selenomethionine alleviates NF-\u03baB-mediated inflammation in bovine mammary epithelial cells induced by Escherichia coli by enhancing autophagy","volume":"110","author":"Tao","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Dong, J., Li, J., Li, J., Cui, L., Meng, X., Qu, Y., and Wang, H. (2019). The proliferative effect of cortisol on bovine endometrial epithelial cells. Reprod. Biol. Endocrinol., 17.","DOI":"10.1186\/s12958-019-0544-1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.theriogenology.2007.04.056","article-title":"The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow","volume":"68","author":"Williams","year":"2007","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1146\/annurev-animal-020518-115227","article-title":"Tolerance and innate immunity shape the development of postpartum uterine disease and the impact of endometritis in dairy cattle","volume":"7","author":"Sheldon","year":"2019","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s12026-025-09600-1","article-title":"Lithospermic acid targeting heat shock protein 90 attenuates LPS-induced inflammatory response via NF-\u03baB signalling pathway in BV2 microglial cells","volume":"73","author":"Guo","year":"2025","journal-title":"Immunol. Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Vermot, A., Petit-H\u00e4rtlein, I., Smith, S.M.E., and Fieschi, F. (2021). NADPH oxidases (NOX): An overview from discovery, molecular mechanisms to physiology and pathology. Antioxidants, 10.","DOI":"10.3390\/antiox10060890"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Islam, S.U., Lee, J.H., Shehzad, A., Ahn, E.M., Lee, Y.M., and Lee, Y.S. (2018). Decursinol angelate inhibits LPS-induced macrophage polarization through modulation of the NF\u03baB and MAPK signaling pathways. Molecules, 23.","DOI":"10.3390\/molecules23081880"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"L445","DOI":"10.1152\/ajplung.00261.2012","article-title":"LPS-mediated endothelial activation in pulmonary endothelial cells: Role of Nox2-dependent IKK-\u03b2 phosphorylation","volume":"304","author":"Menden","year":"2013","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"L639","DOI":"10.1152\/ajplung.00109.2014","article-title":"Lipopolysaccharide induces ICAM-1 expression via a c-Src\/NADPH oxidase\/ROS-dependent NF-\u03baB pathway in human pulmonary alveolar epithelial cells","volume":"310","author":"Cho","year":"2016","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"L297","DOI":"10.1152\/ajplung.00305.2016","article-title":"Dysregulated Nox4 ubiquitination contributes to redox imbalance and age-related severity of acute lung injury","volume":"312","author":"Palumbo","year":"2017","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Augsburger, F., Filippova, A., Rasti, D., Seredenina, T., Lam, M., Maghzal, G., Mahiout, Z., Jansen-D\u00fcrr, P., Knaus, U.G., and Doroshow, J. (2019). Pharmacological characterization of the seven human NOX isoforms and their inhibitors. Redox Biol., 26.","DOI":"10.1016\/j.redox.2019.101272"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s12974-018-1186-5","article-title":"NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation","volume":"15","author":"Huang","year":"2018","journal-title":"J. Neuroinflamm."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kim, T.W., and Lee, H.G. (2024). Anti-inflammatory 8-shogaol mediates apoptosis by inducing oxidative stress and sensitizes radioresistance in gastric cancer. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26010173"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"G99","DOI":"10.1152\/ajpgi.00272.2007","article-title":"NADPH oxidase plays a crucial role in the activation of pancreatic stellate cells","volume":"294","author":"Masamune","year":"2008","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1007\/s12011-023-03992-7","article-title":"Strontium attenuates LPS-induced inflammation via TLR4\/MyD88\/NF-\u03baB pathway in bovine ruminal epithelial cells","volume":"202","author":"Tan","year":"2024","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1007\/s00210-020-01906-1","article-title":"Pyrrolidine dithiocarbamate reduces alloxan-induced kidney damage by decreasing nox4, inducible nitric oxide synthase, and metalloproteinase-2","volume":"393","author":"Pereira","year":"2020","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wang, L., Li, L., Zhao, D., Yuan, H., Zhang, H., Chen, J., Pang, D., Lu, Y., and Ouyang, H. (2024). MYH7 R453C induced cardiac remodelling via activating TGF-\u03b2\/Smad2\/3, ERK1\/2 and Nox4\/ROS\/NF-\u03baB signalling pathways. Open Biol., 14.","DOI":"10.1098\/rsob.230427"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Huang, S.H., Liu, Z.M., Chen, S.J., Tu, P.Y., Tien, Y.C., Lu, C.C., Wang, C.C., Chen, L.M., and Shen, P.C. (2025). MiR-708-5p attenuates osteoarthritis progression via multi-target modulation of the NOX4\/NF-\u03baB axis and cartilage homeostasis. Cartilage, 19476035251361679.","DOI":"10.1177\/19476035251361679"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhu, Z., Zhao, X., OuYang, Q., Wang, Y., Xiong, Y., Cong, S., Zhou, M., Zhang, M., Luo, X., and Cheng, M. (2021). Waterfall forest environment regulates chronic stress via the NOX4\/ROS\/NF-\u03baB signaling pathway. Front. Neurol., 12.","DOI":"10.3389\/fneur.2021.619728"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1017\/S1466252308001588","article-title":"The role of dietary selenium in bovine mammary gland health and immune function","volume":"10","author":"Salman","year":"2009","journal-title":"Anim. Health Res. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zhang, F., Li, X., and Wei, Y. (2023). Selenium and selenoproteins in health. Biomolecules, 13.","DOI":"10.3390\/biom13050799"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1007\/s12011-021-02731-0","article-title":"The antioxidant role of selenium via GPx1 and GPx4 in LPS-induced oxidative stress in bovine endometrial cells","volume":"200","author":"Adeniran","year":"2022","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Campo-Sabariz, J., Garc\u00eda-Vara, A., Moral-Anter, D., Briens, M., Hachemi, M.A., Pinloche, E., Ferrer, R., and Mart\u00edn-Venegas, R. (2022). Hydroxy-selenomethionine, an organic selenium source, increases selenoprotein expression and positively modulates the inflammatory response of LPS-stimulated macrophages. Antioxidants, 11.","DOI":"10.3390\/antiox11101876"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.cellsig.2017.07.012","article-title":"Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K\/Akt pathway inhibition","volume":"38","author":"Yang","year":"2017","journal-title":"Cell. Signal."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1111\/jcmm.17738","article-title":"Selenium suppressed the LPS-induced inflammation of bovine endometrial epithelial cells through NF-\u03baB and MAPK pathways under high cortisol background","volume":"27","author":"Cui","year":"2023","journal-title":"J. Cell. Mol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1007\/s12011-023-03757-2","article-title":"Selenomethionine inhibits NF-\u03baB-mediated inflammatory responses of bovine mammary epithelial cells caused by Klebsiella pneumoniae by increasing autophagic flux","volume":"202","author":"Xu","year":"2024","journal-title":"Biol. Trace Elem. Res."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/9\/789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:33:40Z","timestamp":1760034820000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/9\/789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,22]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["vetsci12090789"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12090789","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,22]]}}}