{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T06:18:40Z","timestamp":1763878720412,"version":"build-2065373602"},"reference-count":67,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cFondi Ateneo\u201d University of Pisa"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Emerging infectious diseases, particularly those of zoonotic origin, often originating from wildlife reservoirs represent a growing threat to global health. Human-driven environmental changes such as habitat fragmentation, climate change, and urban expansion have intensified interactions at the wildlife\u2013domestic animal\u2013human interface, facilitating cross-species viral transmission. Despite their epidemiological importance, systematic virological surveillance of wildlife remains challenging. In this study, we employed shotgun metagenomic sequencing to characterize the virome of wild animals rescued in the Pisa area and hospitalized at the \u201cMario Modenato\u201d Veterinary Teaching Hospital (VTH) at the University of Pisa. Fecal samples collected from injured wildlife admitted between September 2020 and September 2021 were analyzed to detect both known and novel viruses. This approach builds upon previous PCR-based investigations of the same biological material, enabling a more comprehensive assessment of viral diversity. We adopted a shotgun approach for analyzing six sample pools\u2014four were positive for at least one viral target\u2014identifying diverse viral families, including Astroviridae, Circoviridae, Picornaviridae, Adenoviridae, and Retroviridae, in asymptomatic wildlife admitted to a veterinary hospital, highlighting their potential role as reservoirs. Our findings provide insights into the influence of environmental and anthropogenic factors on wildlife virome composition and highlight the value of hospital-based sampling strategies for urban viral surveillance. The results contribute to the development of integrated monitoring and prevention strategies within a One Health framework.<\/jats:p>","DOI":"10.3390\/vetsci12090820","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T14:43:18Z","timestamp":1756219398000},"page":"820","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metagenomic Analysis of the Fecal Virome in Wild Mammals Hospitalized in Pisa, Italy"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6738-0699","authenticated-orcid":false,"given":"Maria Irene","family":"Pacini","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2474-8219","authenticated-orcid":false,"given":"Mario","family":"Forzan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-1803","authenticated-orcid":false,"given":"Micaela","family":"Sgorbini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6738-0354","authenticated-orcid":false,"given":"Dania","family":"Cingottini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3797-8320","authenticated-orcid":false,"given":"Maurizio","family":"Mazzei","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1136\/bmj.331.7527.1214","article-title":"A Walk on the Wild Side\u2014Emerging Wildlife Diseases","volume":"331","author":"Cunningham","year":"2005","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"a739","DOI":"10.4102\/ojvr.v81i2.739","article-title":"Drivers of Disease Emergence and Spread: Is Wildlife to Blame?","volume":"81","author":"Kock","year":"2014","journal-title":"Onderstepoort J. 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