{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:08:30Z","timestamp":1793210910473,"version":"4.5.0"},"reference-count":57,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP19677697"],"award-info":[{"award-number":["AP19677697"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of Theileria and Anaplasma spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera Hyalomma (86.9%; 2711\/3121), Rhipicephalus (8.5%; 266\/3121), Dermacentor (3.2%; 101\/3121), and Haemaphysalis (1.4%; 43\/3121). Morphological identification revealed that ticks of the genus Hyalomma, including Hyalomma scupense (31.7%), Hyalomma asiaticum (27.9%), and Hyalomma anatolicum (19.6%), were the predominant species. Rhipicephalus annulatus was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the cox1 gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of Theileria and Anaplasma species using conventional PCR with primers targeting the 28SrRNA and GroEL genes, respectively. No Anaplasma spp. were detected in the analyzed tick samples. Theileria annulata DNA was identified in seven nymphs of Hy. scupense (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female Rhipicephalus annulatus (0.9%) collected in the Almaty oblast. The overall infection prevalence of Theileria annulata was 7.0% (8\/113).<\/jats:p>","DOI":"10.3390\/vetsci12090901","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T13:03:02Z","timestamp":1758114182000},"page":"901","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Study of Theileria\u00a0annulata and Anaplasma spp. in Ixodid Ticks from Southern Regions of the Republic of Kazakhstan"],"prefix":"10.3390","volume":"12","author":[{"given":"Zaure Z.","family":"Sayakova","sequence":"first","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saltanat A.","family":"Kenessary","sequence":"additional","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8980-8804","authenticated-orcid":false,"given":"Ainur A.","family":"Zhaksylykova","sequence":"additional","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bagzhan M.","family":"Abdimalik","sequence":"additional","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora A.","family":"Kydyrkhanova","sequence":"additional","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8444-3305","authenticated-orcid":false,"given":"Dinara K.","family":"Kamalova","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology LLP, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7100-2711","authenticated-orcid":false,"given":"Anara","family":"Ryskeldina","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology LLP, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3771-423X","authenticated-orcid":false,"given":"Yekaterina O.","family":"Ostapchuk","sequence":"additional","affiliation":[{"name":"Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine M.","family":"Budke","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-7237","authenticated-orcid":false,"given":"Aida M.","family":"Abdybekova","sequence":"additional","affiliation":[{"name":"Kazakh Scientific Research Veterinary Institute LLP, Almaty 050016, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Guglielmone, A.A., and Robbins, R.G. (2018). Hard Ticks (Acari: Ixodida: Ixodidae) Parasitizing Humans, Springer.","DOI":"10.1007\/978-3-319-95552-0"},{"key":"ref_2","first-page":"341","article-title":"Checklist of ixodid ticks (Acari: Ixodidae) of Russia","volume":"54","author":"Tsapko","year":"2020","journal-title":"Parazitologiya"},{"key":"ref_3","first-page":"68","article-title":"Ixodid ticks (Ixodidae) as human ectoparasites in Kyrgyzstan","volume":"1","author":"Fedorova","year":"2021","journal-title":"Vestn. Meditsiny I Obraz."},{"key":"ref_4","unstructured":"Kuklina, T.E. (1976). Fauna of Ixodid Ticks of Uzbekistan, Fan."},{"key":"ref_5","unstructured":"Berdyev, A. (1980). Ecology of Ixodid Ticks of Turkmenistan and Their Role in the Epizootology of Natural Focal Diseases, EurekaMag."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhang, Y.K., Zhang, X.Y., and Liu, J.Z. (2019). Ticks (Acari: Ixodoidea) in China: Geographical distribution, host diversity, and specificity. Arch. Insect Biochem. Physiol., 102.","DOI":"10.1002\/arch.21544"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.70264\/jbr.v1.4.2024.2","article-title":"Ticks of the genus Ixodes Latreille, 1795 (Ixodidae, Ixodinae) in Kyzylorda region","volume":"1","author":"Sayakova","year":"2024","journal-title":"J. Biol. Res."},{"key":"ref_8","first-page":"11069","article-title":"Distribution characteristics of ixodid ticks of the genus Hyalomma Koch, 1844 (Acari, Ixodidae) in the south of Kazakhstan","volume":"8","author":"Sayakova","year":"2021","journal-title":"Nat. Volatiles Essent. Oils"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"129","DOI":"10.35885\/1996-1499-15-1-129-135","article-title":"Migratory birds transporting Hyalomma rufipes Koch, 1844 ticks into southern Russia\u2014the vectors of Crimean-Congo hemorrhagic fever virus: Epidemiological aspect","volume":"1","author":"Tsapko","year":"2022","journal-title":"Russ. J. Biol. Invasions"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102518","DOI":"10.1016\/j.ttbdis.2025.102518","article-title":"Hyalomma rufipes of Asian origin transported to Europe by a migrant bird","volume":"16","author":"Sandor","year":"2025","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_11","unstructured":"Kenessary, S.A., Abdybekova, A.M., Sayakova, Z.Z., Zhaksylykova, A.A., Yelubaeva, A.S., Kydyrkhanova, E.A., and Abdimalik, B.M. (2024). Species diversity of ixodid ticks (Acari, Ixodidae) in the Zhambyl region of the Republic of Kazakhstan. Stud. Wildl. Kyrg., 185\u2013191."},{"key":"ref_12","first-page":"25","article-title":"Ixodid ticks of farm animals in Southern Kazakhstan: Fauna structure, abundance, and epizootological significance","volume":"54","author":"Kulemin","year":"2020","journal-title":"Parazitologiya"},{"key":"ref_13","first-page":"55","article-title":"Distribution of ticks of the genus Dermacentor Koch, 1844 (Ixodidae, Amblyomminae) in the South-Eastern part of Kazakhstan","volume":"5","author":"Sayakova","year":"2019","journal-title":"News Natl. Acad. Sci. Repub. Kazakhstan. Ser. Biol. Med."},{"key":"ref_14","first-page":"38","article-title":"Ticks of the genus Dermacentor Koch, 1844 (Ixodidae, Amblyomminae) in the Kyzylorda region","volume":"3\u20131","author":"Sayakova","year":"2023","journal-title":"Sci. Educ."},{"key":"ref_15","unstructured":"Duysheev, N.A. (1984). Theileriosis of cattle in Northern Kyrgyzstan caused by Theileria orientalis Jakimoff et Soudat-Schenkoff, 1931. [Ph.D. Thesis, Kyrgyz State Technical University (KSTU)]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"277","DOI":"10.4103\/0972-9062.217620","article-title":"First detection of Babesia ovis in Dermacentor spp. in Ardabil area, northwest of Iran","volume":"54","author":"Shahbazi","year":"2017","journal-title":"J. Vector Borne Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kim, Y.J., Seo, J.Y., Park, J.S., Kim, S.Y., Aknazarov, B., Atabekova, N., and Lee, H.I. (2024). Molecular analysis of tick-borne bacterial pathogens from ticks infesting animal hosts in Kyrgyzstan. Microorganisms, 12.","DOI":"10.3390\/microorganisms12061046"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Li, Y., Li, J., Xieripu, J., Rizk, G., Macalanda, M., Gan, A.M., Ren, L., Mohanta, J., Elsayed, U., and Bayin, S. (2024). Molecular detection of Theileria ovis, Anaplasma ovis, and Rickettsia spp. in Rhipicephalus turanicus and Hyalomma anatolicum collected from sheep in Southern Xinjiang, China. Pathogens, 13.","DOI":"10.3390\/pathogens13080680"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ttbdis.2013.09.006","article-title":"PCR-based detection of Theileria annulata in Hyalomma asiaticum ticks in northwestern China","volume":"5","author":"Meng","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_20","first-page":"76","article-title":"Identification of Theileria species in sheep and vector ticks using PCR method in Zabol, Eastern Iran","volume":"13","author":"Zarei","year":"2019","journal-title":"J. Arthropod Borne Dis."},{"key":"ref_21","first-page":"51","article-title":"Distribution and harmfulness of ixodid ticks in the Russian Federation","volume":"1","author":"Glazunov","year":"2014","journal-title":"Veterinariya"},{"key":"ref_22","unstructured":"Doroshchenko, E.K., Lisak, O.V., Rar, V.A., Suntsova, O.V., Savinova, Y.S., and Kozlova, I.V. (2019). Species and genetic diversity of the family Anaplasmataceae revealed in the sympatric zone of ticks of the genera Ixodes, Dermacentor, and Haemaphysalis. Acta Biomed. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1016\/j.ttbdis.2018.07.008","article-title":"Epidemiological study on tropical theileriosis (Theileria annulata infection) in the Egyptian Oases with special reference to the molecular characterization of Theileria spp.","volume":"9","author":"Ahmed","year":"2018","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"101817","DOI":"10.1016\/j.ttbdis.2021.101817","article-title":"Tick distribution and detection of Babesia and Theileria species in Eastern and Southern Kazakhstan","volume":"12","author":"Sang","year":"2021","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_25","first-page":"10","article-title":"Development of a real-time PCR for the identification of Anaplasma marginale in cattle","volume":"2","author":"Ostrovskii","year":"2024","journal-title":"Herald Sci. S. Seifullin Kazakh Agrotech. Res. Univ. Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"87","DOI":"10.52578\/2305-9397-2024-4-1-87-95","article-title":"Epizootic situation and economic damage from bovine theileriosis in the south of Kazakhstan","volume":"1","author":"Mussayeva","year":"2024","journal-title":"Gylym Zhane Bilim"},{"key":"ref_27","unstructured":"(2025, June 16). Livestock Mortality in Ulytau Region. Available online: https:\/\/liter.kz\/padezh-skota-proiskhodit-v-oblasti-ulytau-1658858358\/."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sultankulova, K.T., Shynybekova, G.O., Issabek, A.U., Mukhami, N.N., Melisbek, A.M., Chervyakova, O.V., Kozhabergenov, N.S., Barmak, S.M., Bopi, A.K., and Omarova, Z.D. (2022). The Prevalence of Pathogens among Ticks Collected from Livestock in Kazakhstan. Pathogens, 11.","DOI":"10.3390\/pathogens11101206"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Weixin, Z., Zhumanov, K., Awulibieer, M., Sansyzbay, A., Khizat, S., Meihua, Y., Yuanzhi, W., and Wurelihazi, H. (2025). Molecular detection of piroplasms, Anaplasma, and Ehrlichia species in Kazakhstan. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1533589"},{"key":"ref_30","unstructured":"Filippova, N.A. (1977). Ixodid Ticks of the Subfamily Ixodidae, Bioscience Reports."},{"key":"ref_31","unstructured":"Filippova, N.A. (1997). Ixodid Ticks of the Subfamily Amblyomminae, Bioscience Reports."},{"key":"ref_32","unstructured":"Walker, A.R., Bouattour, A., Camicas, J.-L., Estrada-Pe\u00f1a, A., Horak, I., Abdalla, L., Pegram, R.G., and Preston, P.M. (2014). Ticks of Domestic Animals in Africa: A Guide to Identification of Species, Bioscience Reports."},{"key":"ref_33","first-page":"268","article-title":"On the identification of species and subspecies of the genus Hyalomma (Acari: Ixodidae) of the fauna of Russia and adjacent territories by the larval phase","volume":"41","author":"Apanaskevich","year":"2007","journal-title":"Parasitology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.7150\/ijbs.6081","article-title":"Complete mitochondrial genome sequence data provides genetic evidence that the brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) represents a species complex","volume":"9","author":"Liu","year":"2013","journal-title":"Int. J. Biol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"102078","DOI":"10.1016\/j.ttbdis.2022.102078","article-title":"Theileria and Babesia infection in cattle\u2014First molecular survey in Kazakhstan","volume":"14","author":"Kuibagarov","year":"2023","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kadyrova, M., Ostrovskii, A., Mukanov, K., Kassen, A., Shevtsova, E., Berdikulov, M., and Shevtsov, A. (2024). Molecular characterization of Anaplasma spp. in cattle from Kazakhstan. Pathogens, 13.","DOI":"10.3390\/pathogens13100894"},{"key":"ref_37","unstructured":"National Center for Biotechnology Information (NCBI) (2025, May 21). BLAST: Basic Local Alignment Search Tool, Available online: http:\/\/www.ncbi.nlm.nih.gov\/BLAST."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/molbev\/msae263","article-title":"Molecular Evolutionary Genetics Analysis Version 12 for adaptive and green computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol Biol Evol."},{"key":"ref_39","unstructured":"PubMLST (2025, May 21). Multi-Locus Sequence Typing for Bacteria and Fungi. Available online: https:\/\/pubmlst.org."},{"key":"ref_40","unstructured":"World Organisation for Animal Health (WOAH) (2025, June 16). Theileriosis. Available online: https:\/\/www.woah.org\/en\/disease\/theileriosis\/."},{"key":"ref_41","first-page":"84","article-title":"Prevalence of ticks, vectors of Crimean-Congo hemorrhagic fever virus, in Kazakhstan","volume":"18","author":"Kulemin","year":"2023","journal-title":"Med. Bull. Bashkortostan"},{"key":"ref_42","first-page":"59","article-title":"On the fauna and ecology of ticks collected in Moiynkum steppe of Zhambyl region during 2002\u20132016","volume":"1","author":"Sarsenbayeva","year":"2017","journal-title":"Quar. Zoonotic Infect Kazakhstan"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1310071","DOI":"10.3389\/fepid.2024.1310071","article-title":"Outcome of the entomological monitoring for Crimean-Congo haemorrhagic fever virus in the western and southern regions of Kazakhstan in 2021\u20132022","volume":"4","author":"Nurmakhanov","year":"2024","journal-title":"Front Epidemiol."},{"key":"ref_44","first-page":"43","article-title":"Results of tick testing for detection of Karshi, Tamdy, Issyk-Kul fever, and Syr-Darya Valley fever viruses","volume":"102","author":"Nurmakhanov","year":"2021","journal-title":"Bull. Karaganda Univ. Biol. Med. Geogr. Ser."},{"key":"ref_45","unstructured":"Kurchiev, M.Y., and Manilova, E.A. (2023). Ixodoid ticks\u2014Carriers of transmissible diseases of humans and animals in Tajikistan. Endless Light Sci., 552\u2013555."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ma, R., Li, C., Gao, A., Jiang, N., Li, J., Hu, W., and Feng, X. (2024). Tick species diversity and potential distribution alternation of dominant ticks under different climate scenarios in Xinjiang, China. PLoS Negl. Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0012108"},{"key":"ref_47","first-page":"55","article-title":"Detection of Ehrlichia spp. and Theileria spp. in Hyalomma anatolicum ticks collected in Tajikistan","volume":"24","author":"Kartashov","year":"2020","journal-title":"Vavilovskii Zhurnal Genet Sel."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bulatov, Y., Turyskeldy, S., Abitayev, R., Usembai, A., Sametova, Z., Kondybayeva, Z., Kurmasheva, A., Mazbayeva, D., Kyrgyzbayeva, A., and Shorayeva, K. (2024). Camelpox Virus in Western Kazakhstan: Assessment of the Role of Local Fauna as Reservoirs of Infection. Viruses, 16.","DOI":"10.3390\/v16101626"},{"key":"ref_49","unstructured":"Maikanov, N.S., and Maikanova, F.N. (2025, May 21). Ixodid Ticks of Western Kazakhstan. Steppe Institute of the Ural Branch of the Russian Academy of Sciences. Available online: https:\/\/orensteppe.org\/content\/iksodovye-kleshchi-zapadnogo-kazahstana."},{"key":"ref_50","first-page":"228","article-title":"Epidemiological significance of blood-sucking insects and arachnids in western Kazakhstan","volume":"2","author":"Maikanov","year":"2022","journal-title":"Vestn. WKU"},{"key":"ref_51","unstructured":"Kolonin, G.V. (1983). Global Distribution of Ixodid Ticks. Genera Hyalomma, Aponomma, Amblyomma, Izdatel\u2019stvo \u201cNauka\u201d."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/s10493-023-00893-1","article-title":"Application of DNA barcodes in the genetic diversity of hard ticks (Acari: Ixodidae) in Kazakhstan","volume":"92","author":"Zheng","year":"2024","journal-title":"Exp Appl Acarol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3467","DOI":"10.1007\/s00436-022-07670-w","article-title":"Predicting the potential distribution of the cattle fever tick Rhipicephalus annulatus (Acari: Ixodidae) using ecological niche modeling","volume":"121","author":"Okely","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1007\/s00436-020-06728-x","article-title":"The Rhipicephalus appendiculatus tick vector of Theileria parva is absent from cape buffalo (Syncerus caffer) populations and associated ecosystems in northern Uganda","volume":"119","author":"Obara","year":"2020","journal-title":"Parasitol Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"573","DOI":"10.17221\/1861-VETMED","article-title":"Bacteria of the genus Anaplasma\u2014Characteristics of Anaplasma and their vectors: A review","volume":"53","author":"Rymaszewska","year":"2008","journal-title":"Vet. Med."},{"key":"ref_56","unstructured":"World Organisation for Animal Health (WOAH) (2025, May 21). Bovine Anaplasmosis. Available online: https:\/\/www.woah.org\/en\/disease\/bovine-anaplasmosis."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1196\/annals.1374.068","article-title":"Molecular identification of Rickettsia felis-like bacteria in Haemaphysalis sulcata ticks collected from domestic animals in Southern Croatia","volume":"1078","author":"Duh","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/9\/901\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:47:05Z","timestamp":1760035625000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/9\/901"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,17]]},"references-count":57,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["vetsci12090901"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12090901","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,17]]}}}