{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T17:12:10Z","timestamp":1760721130215,"version":"build-2065373602"},"reference-count":56,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-42-17"],"award-info":[{"award-number":["CARS-42-17"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sichuan Veterinary Medicine, Drug Innovation Group of the China Agricultural Research System","award":["SCCXTD-2021-18"],"award-info":[{"award-number":["SCCXTD-2021-18"]}]},{"name":"Research Interest Training Program","award":["20252195"],"award-info":[{"award-number":["20252195"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The genome of the herpesvirus is a linear double-stranded DNA. The viral genome replicates in the host cell to form a concatemeric DNA, which is then cleaved to produce a unit-length genome. This unit-length genome is packaged into procapsid to produce mature virus particles. The terminase large subunit, pUL15, mediates the cleavage and packaging of viral concatemeric genomes. Duck plague virus (DPV) is a member of the \u03b1 herpesvirus subfamily. Previous studies have demonstrated that the C-terminal region of DPV pUL15 exhibits non-sequence-specific DNA cleavage activity in vitro, but the characteristics of DPV full-length pUL15 remain unclear. In this study, it was determined that the full-length pUL15 exerted non-sequence-specific nuclease activity. Additionally, full-length pUL15 was capable of binding to DNA and hydrolyzing ATP. To analyze the functional domain of DPV pUL15, pUL15 mutants were constructed, expressed, and purified. The results revealed that DNA-binding and ATPase functions of pUL15 were primarily mediated by its N-terminal region, and the nuclease activity was conducted by its C-terminus. The loss of the nuclease activity did not effect on the DNA-binding and ATPase activity. Taken together, this study\u2019s findings demonstrated that DPV pUL15 is a multifunctional enzyme with ATPase, nuclease, and DNA-binding activities. These results will provide important clues for subsequent studies on the function of terminase and the process of viral genome packaging, and provide a foundational basis for the development of broad-spectrum anti-herpesviral drugs targeting the conserved terminase complex, with direct relevance to veterinary medicine.<\/jats:p>","DOI":"10.3390\/vetsci12100992","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T16:13:03Z","timestamp":1760717583000},"page":"992","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Duck Plague Virus Full-Length UL15 Protein Is a Multifunctional Enzyme Which Not Only Possesses Nuclease Activity but Also Exerts ATPase and DNA-Binding Activity"],"prefix":"10.3390","volume":"12","author":[{"given":"Qiao","family":"Yang","sequence":"first","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingshu","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Tian","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anchun","family":"Cheng","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China"},{"name":"Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People\u2019s Republic of China, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.tim.2011.09.001","article-title":"Herpes simplex virus capsid assembly and DNA packaging: A present and future antiviral drug target","volume":"19","author":"Baines","year":"2011","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1099\/jgv.0.000984","article-title":"The C-terminal part 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