{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:01:32Z","timestamp":1763978492659,"version":"3.45.0"},"reference-count":31,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jilin Province Science and Technology Innovation Platform Subsidy Project","award":["YDZJ202302CXJD038"],"award-info":[{"award-number":["YDZJ202302CXJD038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Objective:\u00a0P. mirabilis is a Gram-negative rod-shaped bacterium. In this study, the SH-8 strain of P. mirabilis was isolated from swine-derived samples, and its antibiotic resistance genes (ARGs) and transmission mechanisms were systematically characterized. Methods: The minimum inhibitory concentrations (MICs) of strain SH-8 were determined by the broth microdilution method, with genomic features analyzed through whole-genome sequencing (WGS). Results:\u00a0P. mirabilis SH-8 exhibited resistance to multiple antimicrobial agents. Its genome consists of a single chromosome (3,993,987 bp; GC content 38.8%) and one plasmid. WGS identified 21 distinct ARGs in this strain, along with a class 2\/1 hybrid integron. Furthermore, a heatmap analysis of resistance genes from 29 P. mirabilis isolates of diverse origins revealed a striking similarity between the resistance gene profile of SH-8 and those of other strains. Conclusions: The MDR exhibited by P. mirabilis SH-8 is primarily attributed to integron-mediated resistance gene clusters within its genome, which may pose challenges for treating opportunistic infections caused by this bacterium.<\/jats:p>","DOI":"10.3390\/vetsci12121113","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:02:07Z","timestamp":1763974927000},"page":"1113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Characterization of a Class 2\/1 Hybrid Integron-Driven Multidrug-Resistant Proteus mirabilis SH-8 of Swine Origin"],"prefix":"10.3390","volume":"12","author":[{"given":"Xinyu","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Su","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3405-4846","authenticated-orcid":false,"given":"Nuo","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donghui","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiarui","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanli","family":"Sha","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"},{"name":"Jilin Provincial Key Laboratory of Preventive Veterinary Medicine, Jilin 132101, China"},{"name":"Jilin Province Technology Innovation Center of Pig Ecological Breeding and Disease Prevention and Control, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baishuang","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"},{"name":"Jilin Provincial Key Laboratory of Preventive Veterinary Medicine, Jilin 132101, China"},{"name":"Jilin Province Cross Regional Cooperation Technology Innovation Center of Porcine Main Disease Prevention and Control, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlong","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China"},{"name":"Jilin Provincial Key Laboratory of Preventive Veterinary Medicine, Jilin 132101, China"},{"name":"Jilin Province Technology Innovation Center of Pig Ecological Breeding and Disease Prevention and Control, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1007\/s42770-020-00240-1","article-title":"Proteus mirabilis causing cellulitis in broiler chickens","volume":"51","author":"Sanches","year":"2020","journal-title":"Braz. 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