{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T09:15:22Z","timestamp":1765444522753,"version":"3.46.0"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Project for Regional Innovation of Hubei Province","award":["2024EHA010"],"award-info":[{"award-number":["2024EHA010"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902231"],"award-info":[{"award-number":["31902231"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"College Students\u2019 Innovation and Entrepreneurship Training Program of Yangtze University","award":["Yz2025090"],"award-info":[{"award-number":["Yz2025090"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Background: Individual differences in immune responses to African swine fever virus (ASFV), whether induced by vaccination or natural infection, may be linked to genetic variation in the genes involved in antigen presentation. Methods: A total of nine pigs from the 112-population were selected for RNA-seq analysis. To pinpoint key transcription factors (TFs) regulating gene expression in the lymph nodes, weighted Kendall\u2019s Tau rank correlation analysis was performed to link the TF binding potential with the extent of differential expression of target genes. Results: CD8+ T cells expressing a specific epitope of the ASFV p72 protein (ACD8+) accounted for 41% of the total CD8+ T cells in peripheral blood. A total of 2062 transcripts were identified as differentially expressed across the nine pigs (q-value &lt; 1 \u00d7 10\u22128). Differential expression levels of the target genes for MECP2, ETS1, ZBTB33, ELK4, and E2F4 were significantly correlated with their TF binding potential (p &lt; 0.05). Six SNPs were identified in the promoter region of ELK4. Analysis of the 112-pig population revealed that SNPs at S.-404A&gt;G and S.-668C&gt;T loci were significantly associated with ACD8+ levels (q-value &lt; 0.01). Individuals with the AA genotype at S.-404A&gt;G had significantly higher ACD8+ counts compared to those with AG and GG genotypes (q-value &lt; 0.05). At the S.-668C&gt;T locus, ACD8+ levels were highest in the CC genotype, followed by CT and TT genotypes, with CC showing notably higher ACD8+ counts (q-value &lt; 0.05). Notably, the S.-404A&gt;G site overlaps with potential binding sites for TFs FOXA2, GATAs, and TRPS1, while the S.-668C&gt;T site lies within the binding regions for NR1H3, RARA, VDR, and NR1I3. Conclusion: These mutations may disrupt TFs binding to the ELK4 promoter, potentially reducing ELK4 expression and impairing antigen processing and presentation.<\/jats:p>","DOI":"10.3390\/vetsci12121184","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T08:39:18Z","timestamp":1765442358000},"page":"1184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Regulators for Antigen-Specific CD8+ T Cells in African Swine Fever Virus-Restored Pigs"],"prefix":"10.3390","volume":"12","author":[{"given":"Fanghong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alegria Agostinho","family":"Francisco","sequence":"additional","affiliation":[{"name":"Angola Ministry of Agriculture Animal Husbandry and Veterinary Research Institute, Luanda 1000, Angola"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beneque Alberto","family":"Anzol","sequence":"additional","affiliation":[{"name":"Angola Ministry of Agriculture Animal Husbandry and Veterinary Research Institute, Luanda 1000, Angola"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guopin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tinghua","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0368-1742(21)80031-4","article-title":"On A Form of Swine Fever Occurring in British East Africa (Kenya Colony)","volume":"34","year":"1921","journal-title":"J. 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