{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:16:01Z","timestamp":1787030161512,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Serbian Ministry of Science, Technological Development and Innovation","award":["45-03-136\/2025-03\/200030"],"award-info":[{"award-number":["45-03-136\/2025-03\/200030"]}]},{"name":"PRFU project","award":["D04N02UN140120230001"],"award-info":[{"award-number":["D04N02UN140120230001"]}]},{"name":"PRFU project","award":["DGRSDT\u2013MESRS"],"award-info":[{"award-number":["DGRSDT\u2013MESRS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Bovine viral diarrhea virus (BVDV) poses a serious disease in cattle. Cachexia is a condition marked by weight loss and muscle wasting and is often observed in infected animals. This study reports a case series from Tiaret province, Algeria, aimed at detecting and genetically identifying BVDV in clinically cachectic cattle. A total of 100 cachectic animals from ten herds were examined using serological, molecular, and virological methods, including antibody ELISA, RT-qPCR, and virus isolation in cell culture. Overall, 93.6% of the animals tested were seropositive for BVDV, indicating extensive exposure to the sampled population. Higher antibody rates were observed in adults (\u226512 months) and males compared to younger animals and females, but the differences were not statistically significant. Phylogenetic analysis of the 5\u2032UTR sequences confirmed the presence of the BVDV-1f subtype closely related to sequences reported in Europe. Although BVDV was detected in animals within the sampled herds, the data does not support a causal conclusion that BVDV was the direct cause of cachexia in this case series; rather, its immunosuppressive effects may predispose cattle to secondary infections contributing to wasting. These results are specific to this opportunistic case series from Tiaret province and should be considered exploratory; broader and representative surveillance is required to assess the national epidemiological situation.<\/jats:p>","DOI":"10.3390\/vetsci12121193","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T15:27:20Z","timestamp":1765553240000},"page":"1193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3798-9814","authenticated-orcid":false,"given":"Nacira","family":"Ghenoumat","sequence":"first","affiliation":[{"name":"Laboratory for Improvement and Valorisation of Local Animal Production, Institute of Veterinary Sciences, University of Tiaret, Tiaret 14000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6353-008X","authenticated-orcid":false,"given":"Houari","family":"Hemida","sequence":"additional","affiliation":[{"name":"Laboratory for Improvement and Valorisation of Local Animal Production, Institute of Veterinary Sciences, University of Tiaret, Tiaret 14000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assia","family":"Boumezrag","sequence":"additional","affiliation":[{"name":"Laboratory for Improvement and Valorisation of Local Animal Production, Institute of Veterinary Sciences, University of Tiaret, Tiaret 14000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4335-1690","authenticated-orcid":false,"given":"Dimitrije","family":"Gli\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Virology Department, Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9564-1764","authenticated-orcid":false,"given":"Sofija","family":"\u0160olaja","sequence":"additional","affiliation":[{"name":"Virology Department, Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-3046","authenticated-orcid":false,"given":"Ljubi\u0161a","family":"Veljovi\u0107","sequence":"additional","affiliation":[{"name":"Virology Department, Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1181-6307","authenticated-orcid":false,"given":"Vesna","family":"Mili\u0107evi\u0107","sequence":"additional","affiliation":[{"name":"Virology Department, Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1079\/AHR2005102","article-title":"BVD Control in Europe: Current Status and Perspectives","volume":"6","author":"Moennig","year":"2005","journal-title":"Anim. 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