{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T18:30:39Z","timestamp":1793817039365,"version":"4.6.1"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology of China\u2019s High-end Foreign Experts program","award":["H20250797"],"award-info":[{"award-number":["H20250797"]}],"id":[{"id":"https:\/\/ror.org\/02kv4zf79","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD), P.R. China"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Moringa oleifera (MO) is a versatile non-traditional feed supplement rich in bioactive compounds. The objective of this study was to examine the effects of MO leaf (MOL) polysaccharide (MOLP) intake as a natural product on broiler chicken production and antioxidant indices. A polysaccharide with a molecular weight of 182.989 kDa was isolated from MOL in a previous study. Broiler chickens were allocated at random into four groups receiving varying doses of MOLP (0, 0.1, 0.2 and 0.4 g\/kg feed) for three weeks. Feed intake (FI), average daily feed ingestion (ADFI), feed conversion ratio (FCR), and body weight gain (BWG) were monitored. Serological markers, including total protein (TP), albumin (ALB), globulin (GLO), albumin-to-globulin ratio (ALB\/GLO), creatinine (CREA), as well as the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and the concentrations of malondialdehyde (MDA) were assessed. Results from days 21 to 28 demonstrated that the high dose of MOLP significantly enhanced BWG, ADFI, liver and bursa indices compared to the control group. Additionally, TP and GLO, T-SOD, GSH-Px, T-AOC and MDA levels were elevated (p &lt; 0.05). In conclusion, MOLP supplementation, particularly at 0.4 g\/kg feed, positively impacted broiler chicken growth performance and antioxidant indices, suggesting its potential as a valuable feed additive.<\/jats:p>","DOI":"10.3390\/vetsci12121196","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T09:36:39Z","timestamp":1765791399000},"page":"1196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Impact of Dietary Moringa oleifera Leaf Polysaccharide on Growth Performance and Antioxidant Status in Broiler Chickens"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8993-3507","authenticated-orcid":false,"given":"Hosameldeen Mohamed","family":"Husien","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"College of Veterinary Medicine, Albutana University, Rufaa 22217, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WeiLong","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0459-7946","authenticated-orcid":false,"given":"Raza","family":"Mohai Ud Din","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mudathir Yahia","family":"Abdulrahman","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada N. A. M.","family":"Hassanine","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0380-9712","authenticated-orcid":false,"given":"Mohamed Osman Abdalrahem","family":"Essa","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"College of Veterinary Medicine, Albutana University, Rufaa 22217, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saber Y.","family":"Adam","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hozifa S.","family":"Yousif","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Albutana University, Rufaa 22217, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1314-8066","authenticated-orcid":false,"given":"Ahmed A.","family":"Saleh","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"Animal and Fish Production Department, Faculty of Agriculture (Al-Shatby), Alexandria University, Alexandria City 11865, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"State Key-Laboratory of Sheep Genetic Improvement and Healthy-Production, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0924-8579(00)00145-X","article-title":"Epidemiology of resistance to antibiotics. Links between animals and humans","volume":"14","author":"Stobberingh","year":"2000","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0924-8579(00)00140-0","article-title":"Monitoring of antibiotic resistance in bacteria of animal origin: Epidemiological and microbiological methodologies","volume":"14","author":"Caprioli","year":"2000","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1590\/S1516-635X2003000200001","article-title":"An alternative for antibiotic use in poultry probiotics","volume":"5","author":"Edens","year":"2003","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S1516-635X2004000300008","article-title":"Productive traits of broiler chickens fed diets containing different growth promoters","volume":"6","author":"Pelicano","year":"2004","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/ptr.2023","article-title":"Moringa oleifera: A food plant with multiple medicinal uses","volume":"21","author":"Anwar","year":"2007","journal-title":"Phytother. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mohamed, H.H., Peng, W., Su, H., Zhou, R., Tao, Y., Huang, J., Liu, M., Bo, R., and Li, J. (2022). Moringa oleifera leaf polysaccharide alleviates experimental colitis by inhibiting inflammation and maintaining intestinal barrier. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1055791"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Husien, H.M., Rehman, S.U., Duan, Z., and Wang, M. (2024). Effect of Moringa oleifera Leaf Polysaccharide on the Composition of Intestinal Microbiota in Mice with Dextran Sulfate Sodium-Induced Ulcerative Colitis. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1409026"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12791","DOI":"10.3390\/ijms160612791","article-title":"Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview","volume":"16","author":"Leone","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jssas.2016.02.002","article-title":"Moringa oleifera as a food fortificant: Recent trends and prospects","volume":"17","author":"Oyeyinka","year":"2018","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.psj.2019.10.043","article-title":"Effects of dietary Lycium barbarum polysaccharides on growth performance, digestive enzyme activities, antioxidant status, and immunity of broiler chickens","volume":"99","author":"Long","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.3382\/ps\/pey220","article-title":"Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers","volume":"97","author":"Wu","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ijbiomac.2014.01.020","article-title":"Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L","volume":"65","author":"Zhang","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.5713\/ajas.2007.1453","article-title":"Dietary supplementation with Acanthopanax senticosus extract modulates cellular and humoral immunity in weaned piglets","volume":"20","author":"Kong","year":"2007","journal-title":"Asian-Aust. J. Anim. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.ijbiomac.2013.04.067","article-title":"Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity","volume":"59","author":"Zhao","year":"2013","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1111\/asj.12528","article-title":"Polysaccharides from Acanthopanax senticosus enhances intestinal integrity through inhibiting TLR4\/NF-\u03baB signaling pathways in lipopolysaccharide-challenged mice","volume":"87","author":"Han","year":"2016","journal-title":"Anim. Sci. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jff.2017.03.053","article-title":"Polysaccharide from Lycium barbarum L. leaves enhances absorption of endogenous calcium, and elevates cecal calcium transport protein levels and serum cytokine levels in rats","volume":"33","author":"Ren","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.autrev.2013.06.003","article-title":"Targets for new immunomodulation strategies in inflammatory bowel disease","volume":"13","author":"Monteleone","year":"2014","journal-title":"Autoimmun. Rev."},{"key":"ref_18","unstructured":"Bush, B. (1992). Interpretation of Laboratory Results for Small Animal Clinicians, CABI."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.livsci.2013.08.012","article-title":"Effect of Feeding Graded Levels of Moringa stenopetala Leaf Meal on Growth Performance, Carcass Traits and Some Serum biochemical Parameters of Koekoek","volume":"157","author":"Melesse","year":"2013","journal-title":"Livest. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1093\/ps\/86.9.1955","article-title":"Effect of polysavone (alfalfa extract) on abdominal fat deposition and immunity in broiler chickens","volume":"86","author":"Dong","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3923\/jpt.2009.160.166","article-title":"Protective effect of Moringa oleifera Lam. and Lannea kerstingli extracts against Cadnum and Ethanol-induced lipid peroxidation","volume":"4","author":"Diallo","year":"2009","journal-title":"J. Pharmacol. Toxicol."},{"key":"ref_22","first-page":"55","article-title":"Research progress on degradation methods and product properties of plant polysaccharides","volume":"38","author":"Ji","year":"2023","journal-title":"J. Light Ind."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/jpn.12634","article-title":"Effect of Moringa oleifera leaf powder supplementation on growth performance and intestinal morphology in broiler chickens","volume":"1","author":"Khan","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"378","DOI":"10.3923\/javaa.2011.378.384","article-title":"Effect of different levels of probiotic and prebiotic on performance and carcass characteristics of broiler chickens","volume":"10","author":"Falaki","year":"2011","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.livsci.2014.01.001","article-title":"Effect of Moringa oleifera Leaf Meal on growth performance, apparent digestibility, digestive organ size and carcass yield in broiler chickens","volume":"161","author":"Nkukwana","year":"2014","journal-title":"Livest. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4081\/ijas.2010.e12","article-title":"Effects of dietary supplementation of Aspergillus originated prebiotic (Fermacto) on performance and small intestinal morphology of broiler chickens fed diluted diets","volume":"9","author":"Navidshad","year":"2010","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Song, Q., Zou, Z., Chen, X., Ai, G., Xiong, P., Song, W., Liu, G., Zheng, A., and Chen, J. (2024). Effect of Moringa oleifera Leaf Powder Supplementation on Growth Performance, Digestive Enzyme Activity, Meat Quality, and Cecum Microbiota of Ningdu Yellow Chickens. Agriculture, 14.","DOI":"10.3390\/agriculture14091523"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4338","DOI":"10.1111\/are.15932","article-title":"Effects of Moring oleifera based diets on growth, immunological responses, liver antioxidant biomarkers and expression of immune-related genes in Nile tilapia (Oreochromis niloticus) raised in hapa-in-pond system","volume":"53","author":"Hendam","year":"2022","journal-title":"Aquac. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.tifs.2023.05.013","article-title":"Moringa oleifera Lam. leaves as new raw food material: A review of its nutritional composition, functional properties, and comprehensive application","volume":"138","author":"Yang","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.sajb.2021.05.033","article-title":"Antioxidant and anti-inflammatory properties of novel peptides from Moringa oleifera Lam. leaves","volume":"141","author":"Heredia","year":"2021","journal-title":"S. Afr. J. Bot."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tran, H.-L., Chen, Y.-S., Hung, H.-W., Shih, B.-L., Lee, T.-Y., Yen, C.-H., and Lin, J.-B. (2024). Diet Supplementation with Prinsepiae nux Extract in Broiler Chickens: Its Effect on Growth Performance and Expression of Antioxidant, Pro-Inflammatory, and Heat Shock Protein Genes. Animals, 14.","DOI":"10.3390\/ani14010073"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chen, X., Zeng, D., Zeng, X., and Zeng, Q. (2024). Effects of Complex Antioxidants Added to Chicken Diet on Growth Performance, Serum Biochemical Indices, Meat Quality, and Antioxidant Capacity. Animals, 14.","DOI":"10.3390\/ani14030360"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Yang, C., Wang, S., Li, Q., Zhang, R., Xu, Y., and Feng, J. (2024). Effects of Probiotic Lactiplantibacillus plantarum HJLP-1 on Growth Performance, Selected Antioxidant Capacity, Immune Function Indices in the Serum, and Cecal Microbiota in Broiler Chicken. Animals, 14.","DOI":"10.3390\/ani14050668"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wu, H., Wang, S., Xie, J., Ji, F., Peng, W., Qian, J., Shen, Q., and Hou, G. (2024). Effects of Dietary Lycopene on the Growth Performance, Antioxidant Capacity, Meat Quality, Intestine Histomorphology, and Cecal Microbiota in Broiler Chickens. Animals, 14.","DOI":"10.3390\/ani14020203"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Soren, S., Mandal, G.P., Mondal, S., Pradhan, S., Mukherjee, J., Banerjee, D., Pakhira, M.C., Mondal, A., and Nsereko, V. (2024). Efficacy of Saccharomyces cerevisiae Fermentation Product and Probiotic Supplementation on Growth Performance, Gut Microflora and Immunity of Broiler Chickens. Animals, 14.","DOI":"10.20944\/preprints202401.0277.v1"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Chen, X., Li, Y., Zheng, A., Wang, Z., Wei, X., Li, S., Purba, A., Chen, Z., and Liu, G. (2024). Dietary Replacement of Soybean Meal with Zanthoxylum bungeanum Seed Meal on Growth Performance, Blood Parameters, and Nutrient Utilization in Broiler Chickens. Animals, 14.","DOI":"10.3390\/ani14101420"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Adil, S., Banday, M.T., Hussain, S.A., Wani, M.A., Al-Olayan, E., Patra, A.K., Rasool, S., Gani, A., Sheikh, I.U., and Khan, A.A. (2024). Impact of Nanoencapsulated Rosemary Essential Oil as a Novel Feed Additive on Growth Performance, Nutrient Utilization, Carcass Traits, Meat Quality and Gene Expression of Broiler Chicken. Foods, 13.","DOI":"10.3390\/foods13101515"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, P., Wei, Q., Zhang, C., Pan, H., Li, J., Ji, P., Ma, Y., Dou, T., Wang, Y., and Li, Q. (2024). Effect of Rosemary on Growth Performance, Meat Quality, Fatty Acid Content, Intestinal Flora, and Antioxidant Capacity of Broilers. Animals, 14.","DOI":"10.3390\/ani14172480"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mendes, A.R., Sp\u00ednola, M.P., Lordelo, M., and Prates, J.A.M. (2024). Impact of Chlorella vulgaris Intake Levels on Performance Parameters and Blood Health Markers in Broiler Chickens. Vet. Sci., 11.","DOI":"10.3390\/vetsci11070290"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Lin, X., Ruan, D., Lin, Z., Xiong, T., Zhang, S., Fan, Q., Dong, X., Deng, Y., Jiang, Z., and Jiang, S. (2024). Effects of L-Methionine and DL-Methionine on Growth Performance, Methionine-Metabolizing Enzyme Activities, Feather Traits, and Intestinal Morphology of Medium-Growing, Yellow-Feathered Chickens between 1 and 30 Days of Age. Animals, 14.","DOI":"10.3390\/ani14142135"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhang, H., Huang, L., Hu, S., Qin, X., and Wang, X. (2023). Moringa oleifera Leaf Powder as New Source of Protein-Based Feedstuff Improves Growth Performance and Cecal Microbial Diversity of Broiler Chicken. Animals, 13.","DOI":"10.3390\/ani13061104"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/12\/1196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:08:25Z","timestamp":1765966105000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/12\/1196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,13]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["vetsci12121196"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12121196","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,13]]}}}