{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:19:06Z","timestamp":1793823546723,"version":"4.6.1"},"reference-count":90,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study aimed to evaluate the efficacy of oregano essential oil (OEO) in improving the production performance, health, and welfare of late-phase laying hens raised under commercial farm conditions by analyzing its effect on performance metrics and metabolic and endocrine profiles. Daily performance data for approximately 7884 Hy-Line Brown layers divided into two commercial flocks, one consisting of 96-week-old hens (n = 3849) and the other of 79-week-old hens (n = 4035), were recorded before (Pre-OEO Tx), during (OEO Tx-Week) and one week (Post-OEO Tx Week) following the week of water supplementation with commercial oregano essential oil (5%) of Origanum heracleoticum containing carvacrol (79.75%) as the main component (300 mL of product\/1000 L of water). The results show a significant improvement in hen-day egg production (HDEP) during treatment (p &lt; 0.05), a significant decrease in daily mortality one week after the cessation of treatment, mainly in the youngest hens (p &lt; 0.05), and a reduction in feed conversion rate (p &lt; 0.05). The general model (GLM) analysis of data from blood samples collected before and after OEO addition showed a significant decrease in plasma levels of procalcitonin (PCT), calcium, albumin (p &lt; 0.05), and aspartate aminotransferase (AST) (p &lt; 0.01). In contrast, a significant increase in estradiol, total protein globulin (p &lt; 0.01), and phosphorus levels (p &lt; 0.05) was recorded. The changes in endocrine profiles were significantly related to a restoration of calcium\u2013phosphorus balance and a decrease in hepatic activity of AST and gamma glutamyl transferase (GGT). These results reveal the investigative value of PCT, in conjunction with metabolic profiling and reproductive hormones, for evaluating the effectiveness of phytogenic additives. Further studies are suggested to determine whether essential oil components can improve health and production performances of laying hens by a potential concurrent modulation of their metabolism, inflammatory response, and reproductive axis function.<\/jats:p>","DOI":"10.3390\/vetsci12121213","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T11:08:28Z","timestamp":1766056108000},"page":"1213","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolic and Endocrine Markers of Oregano Essential Oil Effects on Antibacterial Immunity, Reproductive Function, Nutritional Status, and Production Performance of Late-Phase Laying Hens"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5659-2699","authenticated-orcid":false,"given":"Samira","family":"Hadef","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, Faculty of Nature and Life Sciences, Chadli Bendjedid University of El Tarf, P.O. Box 73, El-Tarf 36000, Algeria"},{"name":"Laboratory of Development and Control of Hospital Pharmaceutical Preparations, Faculty of Medicine, Badji Mokhtar University of Annaba, Annaba 23000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawel","family":"Lezzar","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Sciences, Fr\u00e8res Mentouri Constantine I University, Constantine 25100, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3284-1838","authenticated-orcid":false,"given":"Mohamed Walid","family":"Hamlaoui","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Faculty of Natural and Life Sciences, Earth and Universe Sciences, Mohamed Khider University of Biskra, P.O. Box 145 RP, Biskra 07000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-5956","authenticated-orcid":false,"given":"Ahmed","family":"Hadef","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Faculty of Nature and Life Sciences, Chadli Bendjedid University of El Tarf, P.O. Box 73, El-Tarf 36000, Algeria"},{"name":"Laboratory of Development and Control of Hospital Pharmaceutical Preparations, Faculty of Medicine, Badji Mokhtar University of Annaba, Annaba 23000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"ref_1","unstructured":"FAO (2025). FAOSTAT: Crops and Livestock Products 2025. FAOSTAT, FAO. Available online: https:\/\/www.fao.org\/faostat\/en\/#data\/QCL."},{"key":"ref_2","unstructured":"Alloui, O., and Hadef, S. (2008, January 7\u201310). Influence of Probiotic Feeding on the Performance and the Intestinal Microflora of Broiler Chicken. Proceedings of the 1st Mediterranean Summit of WPSA, Advances and Challenges in Poultry Science, Chalkidiki, Greece."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Naeem, M., and Bourassa, D. (2025). Probiotics in Poultry: Unlocking Productivity Through Microbiome Modulation and Gut Health. Microorganisms, 13.","DOI":"10.3390\/microorganisms13020257"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Basiouni, S., Tellez-Isaias, G., Latorre, J.D., Graham, B.D., Petrone-Garcia, V.M., El-Seedi, H.R., Yal\u00e7\u0131n, S., El-Wahab, A.A., Visscher, C., and May-Simera, H.L. (2023). Anti-Inflammatory and Antioxidative Phytogenic Substances against Secret Killers in Poultry: Current Status and Prospects. Vet. Sci., 10.","DOI":"10.3390\/vetsci10010055"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Anagnostopoulos, E.C., Brouklogiannis, I.P., Griela, E., Paraskeuas, V.V., and Mountzouris, K.C. (2023). Phytogenic Effects on Layer Production Performance and Cytoprotective Response in the Duodenum. Animals, 13.","DOI":"10.3390\/ani13020294"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.japr.2019.12.001","article-title":"Production Performance, Egg Quality, and Small Intestine Histomorphology of the Laying Hens Supplemented with Phytogenic Feed Additive","volume":"29","author":"Sharma","year":"2020","journal-title":"J. Appl. Poult. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tran Nguyen Minh, H., Kuo, T.-F., Lin, W.-Y., Peng, T.-C., Yang, G., Lin, C.-Y., Chang, T.-H., Yang, Y.-L., Ho, C.-H., and Ou, B.-R. (2023). A Novel Phytogenic Formulation, EUBIO-BPSG, as a Promising One Health Approach to Replace Antibiotics and Promote Reproduction Performance in Laying Hens. Bioengineering, 10.","DOI":"10.3390\/bioengineering10030346"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Darmawan, A., Hermana, W., Suci, D., Mutia, R., Jayanegara, A., and Ozturk, E. (2022). Dietary Phytogenic Extracts Favorably Influence Productivity, Egg Quality, Blood Constituents, Antioxidant and Immunological Parameters of Laying Hens: A Meta-Analysis. Animals, 12.","DOI":"10.3390\/ani12172278"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rabelo-Ruiz, M., Ariza-Romero, J.J., Zurita-Gonz\u00e1lez, M.J., Mart\u00edn-Platero, A.M., Ba\u00f1os, A., Maqueda, M., Valdivia, E., Mart\u00ednez-Bueno, M., and Peralta-S\u00e1nchez, J.M. (2021). Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum. Animals, 11.","DOI":"10.3390\/ani11020448"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zinno, P., Guantario, B., Lombardi, G., Ranaldi, G., Finamore, A., Allegra, S., Mammano, M.M., Fascella, G., Raffo, A., and Roselli, M. (2023). Chemical Composition and Biological Activities of Essential Oils from Origanum Vulgare Genotypes Belonging to the Carvacrol and Thymol Chemotypes. Plants, 12.","DOI":"10.3390\/plants12061344"},{"key":"ref_11","first-page":"57","article-title":"Huile essentielle d\u2019Origan vulgaire","volume":"61","year":"2022","journal-title":"Actual. Pharm."},{"key":"ref_12","first-page":"83","article-title":"In Vivo Effect of Origanum Vulgare Essential Oil on Microbiological Quality of Raw Milk from Subclinical Mastitis Affected Cows","volume":"67","author":"Hamlaoui","year":"2021","journal-title":"Agric. For."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.14202\/vetworld.2024.197-206","article-title":"Effects of Essential Oils on Egg Production and Feed Efficiency as Influenced by Laying Hen Breed: A Meta-Analysis","volume":"17","author":"Darmawan","year":"2024","journal-title":"Vet. World"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Orzuna-Orzuna, J.F., and Lara-Bueno, A. (2023). Essential Oils as a Dietary Additive for Laying Hens: Performance, Egg Quality, Antioxidant Status, and Intestinal Morphology: A Meta-Analysis. Agriculture, 13.","DOI":"10.3390\/agriculture13071294"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gao, F., Zhang, L., Li, H., Xia, F., Bai, H., Piao, X., Sun, Z., Cui, H., and Shi, L. (2022). Dietary Oregano Essential Oil Supplementation Influences Production Performance and Gut Microbiota in Late-Phase Laying Hens Fed Wheat-Based Diets. Animals, 12.","DOI":"10.21203\/rs.3.rs-1342979\/v1"},{"key":"ref_16","first-page":"85","article-title":"The Effects of Drinking Water Supplemented with Essential Oils on Performance, Egg Quality and Egg Yolk Fatty Acid Composition in Laying Hens","volume":"44","year":"2018","journal-title":"Acta Vet. Eurasia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s40104-021-00600-3","article-title":"Dietary Oregano Essential Oil Supplementation Improves Intestinal Functions and Alters Gut Microbiota in Late-Phase Laying Hens","volume":"12","author":"Feng","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_18","first-page":"7","article-title":"Evaluation of Bacteriostatic Effect of Rosemary and Oregano Essential Oils Against a Non-Pathogenic Surrogate of Salmonella spp. (E. coli ATCC 9637)","volume":"40","author":"Magtalas","year":"2024","journal-title":"Biol. Life Sci. Forum"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Xian, L., Wang, Y., Peng, D., Zang, L., Xu, Y., Wu, Y., Li, J., and Feng, J. (2024). Dietary Oregano Oil Supplementation Improved Egg Quality by Altering Cecal Microbiota Function in Laying Hens. Animals, 14.","DOI":"10.3390\/ani14223235"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1399\/eps.2019.290","article-title":"Effects of Oregano Essential Oil (Origanum syriacum L.) on Performance, Egg Quality, Intestinal Morphology and Oxidative Stress in Laying Hens","volume":"83","author":"Gul","year":"2019","journal-title":"Eur. Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1080\/00071668.2024.2326886","article-title":"The Impact of Dietary Oregano Essential Oil Supplementation on Fatty Acid Composition and Lipid Stability in Eggs Stored at Room Temperature","volume":"65","author":"Johnson","year":"2024","journal-title":"Br. Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"208","DOI":"10.5513\/JCEA01\/16.1.1566","article-title":"Influence of Dietary Zinc and Vitamin C Supplementation on Some Blood Biochemical Parameters and Egg Production in Free-Range Laying Hens","volume":"16","author":"Gerzilov","year":"2015","journal-title":"J. Cent. Eur. Agric."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.14202\/vetworld.2015.1340-1345","article-title":"Biochemical Studies in Experimentally Escherichia coli Infected Broiler Chicken Supplemented with Neem (Azadirachta indica) Leaf Extract","volume":"8","author":"Sharma","year":"2015","journal-title":"Vet. World"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ye, M., Wei, C., Khalid, A., Hu, Q., Yang, R., Dai, B., Cheng, H., and Wang, Z. (2020). Effect of Bacillus Velezensis to Substitute In-Feed Antibiotics on the Production, Blood Biochemistry and Egg Quality Indices of Laying Hens. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02570-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100090","DOI":"10.1016\/j.anopes.2024.100090","article-title":"The Effects of Dietary Oregano Essential Oil on Production, Blood Parameters, and Egg Quality of Laying Hens during the Early Lay Phase","volume":"4","author":"Johnson","year":"2025","journal-title":"Anim.-Open Space"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e70150","DOI":"10.1002\/vms3.70150","article-title":"The Impact of Dietary Encapsulated Fennel Seed (Foeniculum vulgare Mill.) Essential Oil Inclusion Levels on Performance, Serum Hormone Profiles, and Expression of Reproductive Axis-Related Genes in the Early and Late Laying Phases of Hens","volume":"11","author":"Denli","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1086\/421997","article-title":"Serum Procalcitonin and C-Reactive Protein Levels as Markers of Bacterial Infection: A Systematic Review and Meta-Analysis","volume":"39","author":"Simon","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_28","first-page":"39","article-title":"La procalcitonine (PCT): Un outil diagnostique et de strat\u00e9gie th\u00e9rapeutique","volume":"2011","author":"Wolff","year":"2011","journal-title":"Rev. Francoph. Des. Lab."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Battaglia, F., Meucci, V., Tognetti, R., Bonelli, F., Sgorbini, M., Lubas, G., Pretti, C., and Intorre, L. (2020). Procalcitonin Detection in Veterinary Species: Investigation of Commercial ELISA Kits. Animals, 10.","DOI":"10.3390\/ani10091511"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1016\/j.psj.2019.10.049","article-title":"Dietary L-Arginine Supplementation Ameliorates Inflammatory Response and Alters Gut Microbiota Composition in Broiler Chickens Infected with Salmonella enterica Serovar Typhimurium","volume":"99","author":"Zhang","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s40104-019-0371-4","article-title":"In Vivo and in Vitro Protective Effect of Arginine against Intestinal Inflammatory Response Induced by Clostridium perfringens in Broiler Chickens","volume":"10","author":"Zhang","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_32","unstructured":"Hy-Line International (2024). Online Management Guide for Hy-Line Brown Commercial Layers, Hy-Line International. Available online: https:\/\/www.hyline.com\/filesimages\/Hy-Line-Products\/Hy-Line-Product-PDFs\/Brown\/BRN%20STD%20ENG.pdf."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gautron, J., Stapane, L., Le Roy, N., Nys, Y., Rodriguez-Navarro, A.B., and Hincke, M.T. (2021). Correction to: Avian Eggshell Biomineralization: An Update on Its Structure, Mineralogy and Protein Tool Kit. BMC Mol. Cell Biol., 22.","DOI":"10.1186\/s12860-021-00351-z"},{"key":"ref_34","unstructured":"(2016). Proper Collection and Handling of Diagnostic Samples Part One: Serology and Blood Collection, Hy-Line International. Available online: https:\/\/www.hyline.com\/Upload\/Resources\/TU%20SER1%20ENG.pdf."},{"key":"ref_35","unstructured":"Chorfi, Y., and Venne, D. (2015). Biochimie Sanguine Chez Les Oiseaux. Manuel de Pathologie Aviaire, AFAS."},{"key":"ref_36","unstructured":"Kaneko, J.J., Harvey, J.W., and Bruss, M. (2008). Veterinary Clinical Biochemistry of Domestic Animals, London Academic Press."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"13","DOI":"10.20870\/productions-animales.2013.26.1.3131","article-title":"Utilisation Des Huiles Essentielles En Alimentation Des Volailles.2. Hypoth\u00e8ses Sur Les Modes d\u2019action Impliqu\u00e9s Dans Les Effets Observ\u00e9s","volume":"26","author":"Gabriel","year":"2013","journal-title":"INRA Prod. Anim."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e20170901","DOI":"10.1590\/0001-3765201920170901","article-title":"Oregano Essential Oil (Origanum vulgare) to Feed Laying Hens and Its Effects on Animal Health","volume":"91","author":"Migliorini","year":"2019","journal-title":"An. Acad. Bras. Ci\u00eanc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"575","DOI":"10.22358\/jafs\/66216\/2011","article-title":"Effects of Dietary Herbal Essential Oil Mixture and Organic Acid Preparation on Laying Traits, Gastrointestinal Tract Characteristics, Blood Parameters and Immune Response of Laying Hens in a Hot Summer Season","volume":"20","author":"Wellmann","year":"2011","journal-title":"J. Anim. Feed Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.3382\/ps\/pew140","article-title":"Comparative Evaluation of Dietary Supplementation with Mannan Oligosaccharide and Oregano Essential Oil in Forced Molted and Fully Fed Laying Hens between 82 and 106 Weeks of Age","volume":"95","author":"Bozkurt","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"102409","DOI":"10.1016\/j.jtherbio.2019.102409","article-title":"Oregano Essential Oil in the Diet of Laying Hens in Winter Reduces Lipid Peroxidation in Yolks and Increases Shelf Life in Eggs","volume":"85","author":"Migliorini","year":"2019","journal-title":"J. Therm. Biol."},{"key":"ref_42","unstructured":"Abdel-Wareth, A., Ismail, Z., and S\u00fcdekum, K. (2013). Effects of Thyme and Oregano on Performance and Egg Quality Characteristics of Laying Hens. World\u2019s Poult. Sci. J., 1\u20136."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1071\/AN19319","article-title":"Changes in Performance, Egg Quality and Blood Parameters of Laying Hens Fed Selenium and Oregano Oil","volume":"60","author":"Reshadi","year":"2020","journal-title":"Anim. Prod. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"595","DOI":"10.14202\/vetworld.2021.595-602","article-title":"Effects of Oregano (Lippia origanoides) Essential Oil Supplementation on the Performance, Egg Quality, and Intestinal Morphometry of Isa Brown Laying Hens","volume":"14","author":"Ramirez","year":"2021","journal-title":"Vet. World"},{"key":"ref_45","first-page":"143","article-title":"The Effect of Feeding Rosemary, Oregano, Saffron and \u03b1-Tocopheryl Acetate on Hen Performance and Oxidative Stability of Eggs","volume":"35","author":"Botsoglou","year":"2005","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_46","first-page":"48","article-title":"The Influence of Oregano Essential Oil on Egg Quality and Egg Shell Contamination of Laying Hens Kept in Furnished Cages","volume":"62","author":"Denli","year":"2019","journal-title":"Sci. Pap. Ser. D Anim. Sci."},{"key":"ref_47","first-page":"8","article-title":"The Effect of Essential Oils on Performance of Laying Hens","volume":"48","author":"Fit","year":"2015","journal-title":"Sci. Pap. Anim. Sci. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Johnson, A.M., Anderson, G., Arguelles-Ramos, M., and Ali, A.A.B. (2022). Effect of Dietary Essential Oil of Oregano on Performance Parameters, Gastrointestinal Traits, Blood Lipid Profile, and Antioxidant Capacity of Laying Hens during the Pullet Phase. Front. Anim. Sci., 3.","DOI":"10.3389\/fanim.2022.1072712"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1093\/ps\/78.11.1542","article-title":"Laying Hen Productivity as Affected by Energy, Supplemental Fat, and Linoleic Acid Concentration of the Diet","volume":"78","author":"Grobas","year":"1999","journal-title":"Poult. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Weeks, C.A., Lambton, S.L., and Williams, A.G. (2016). Implications for Welfare, Productivity and Sustainability of the Variation in Reported Levels of Mortality for Laying Hen Flocks Kept in Different Housing Systems: A Meta-Analysis of Ten Studies. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146394"},{"key":"ref_51","first-page":"179","article-title":"Survival Analysis of White Leghorn Laying Hens in the Early and Late Production Period","volume":"82","author":"Lamuno","year":"2017","journal-title":"Agric. Conspec. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1751-0147-51-3","article-title":"Causes of Mortality in Laying Hens in Different Housing Systems in 2001 to 2004","volume":"51","author":"Fossum","year":"2009","journal-title":"Acta Vet. Scand."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"339","DOI":"10.2754\/avb200776030339","article-title":"Effects of Housing Systems on Biochemical Indicators of Blood Plasma in Laying Hens","volume":"76","author":"Zapletal","year":"2007","journal-title":"Acta Vet. Brno"},{"key":"ref_54","first-page":"311","article-title":"N\u00edveis S\u00e9ricos de Enzimas Hep\u00e1ticas Em Poedeiras Comerciais No Pr\u00e9-Pico e Pico de Produ\u00e7\u00e3o de Ovos","volume":"38","author":"Ribeiro","year":"2018","journal-title":"Acta Sci. Vet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/jpn.12659","article-title":"Serum Biochemical Profile, Enzymatic Activity and Lipid Peroxidation in Organs of Laying Hens Fed Diets Containing Cashew Nut Shell Liquid","volume":"102","author":"Braz","year":"2018","journal-title":"Anim. Physiol. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.psj.2020.10.039","article-title":"Determination of Selected Biochemical Parameters in Blood Serum and Egg Quality of Czech and Slovak Native Hens Depending on the Housing System and Hen Age","volume":"100","author":"Kraus","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_57","unstructured":"Scanes, C.G., and Dridi, S. (2021). Sturkie\u2019s Avian Physiology, Academic Press."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gao, Z., Zheng, J., and Xu, G. (2025). Molecular Mechanisms and Regulatory Factors Governing Feed Utilization Efficiency in Laying Hens: Insights for Sustainable Poultry Production and Breeding Optimization. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26136389"},{"key":"ref_59","unstructured":"Kaspers, B., Schat, K.A., G\u00f6bel, T., and Vervelde, L. (2021). Avian Immunology, Academic Press."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/pmic.200700790","article-title":"The Chicken Egg Yolk Plasma and Granule Proteomes","volume":"8","author":"Mann","year":"2008","journal-title":"Proteomics"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Jiang, X., Mu, H., Hsieh, Y.-H.P., and Rao, Q. (2022). Isolation and Characterization of Chicken Serum Albumin (Hen Egg Alpha-Livetin, Gal d 5). Foods, 11.","DOI":"10.3390\/foods11111637"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Quattrone, A., Picozzi, I., Lubian, E., Fehri, N.E., Menchetti, L., Barbato, O., Vigo, D., Agradi, S., Sul\u00e7e, M., and Faustini, M. (2025). Blood Glucose in Birds: Another Way to Think About \u201cNormal\u201d Glycemia and Diabetes Mellitus in Animals. Diversity, 17.","DOI":"10.3390\/d17050355"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Schokker, D., Visscher, J., and Woelders, H. (2021). Changes in Blood Metabolites, Intestinal Microbiota Composition and Gene Expression of 95 Weeks Old Laying Hens Differing in Egg Production and Egg Breaking Strength. Animals, 11.","DOI":"10.3390\/ani11113012"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Gloux, A., Duclos, M.J., Brionne, A., Bourin, M., Nys, Y., and R\u00e9hault-Godbert, S. (2019). Integrative Analysis of Transcriptomic Data Related to the Liver of Laying Hens: From Physiological Basics to Newly Identified Functions. BMC Genom., 20.","DOI":"10.1186\/s12864-019-6185-0"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Zaefarian, F., Abdollahi, M., Cowieson, A., and Ravindran, V. (2019). Avian Liver: The Forgotten Organ. Animals, 9.","DOI":"10.3390\/ani9020063"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Wang, Z., Wang, D., Jiang, K., Guo, Y., Li, Z., Jiang, R., Han, R., Li, G., Tian, Y., and Li, H. (2021). A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes from Pre-Laying to Peak-Laying Stage in Liver of Hens (Gallus gallus). Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.700669"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"4107326","DOI":"10.1155\/2024\/4107326","article-title":"Stress-Related Gene Expression in Liver Tissues from Laying Hens Housed in Conventional Cage and Cage-Free Systems in the Tropics","volume":"2024","year":"2024","journal-title":"Vet. Med. Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"740","DOI":"10.5713\/ab.21.0275","article-title":"Dietary Encapsulated Essential Oil Mixture Influence on Apparent Nutrient Digestibility, Serum Metabolic Profile, Lymphocyte Histochemistry and Intestinal Morphology of Laying Hens","volume":"35","author":"Arslan","year":"2022","journal-title":"Anim. Biosci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"101478","DOI":"10.1016\/j.psj.2021.101478","article-title":"Age-Related Changes in Liver Metabolism and Antioxidant Capacity of Laying Hens","volume":"100","author":"Gu","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"102217","DOI":"10.1016\/j.psj.2022.102217","article-title":"Transcriptomic Analysis of the Liver in Aged Laying Hens with Different Eggshell Strength","volume":"102","author":"Han","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"W\u00f3jcik, M., Grabowski, S., Jarosz, \u0141.S., Szymczak, B., Longo, V., Della Croce, C.M., Hejdysz, M., Cie\u015blak, A., Gruszczy\u0144ski, K., and Marek, A. (2024). Liver Antioxidant Capacity and Steatosis in Laying Hens Exposed to Various Quantities of Lupin (Lupinus angustifolius) Seeds in the Diet. Antioxidants, 13.","DOI":"10.3390\/antiox13020251"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Matera, R., Lucchi, E., and Valgimigli, L. (2023). Plant Essential Oils as Healthy Functional Ingredients of Nutraceuticals and Diet Supplements: A Review. Molecules, 28.","DOI":"10.3390\/molecules28020901"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"6287","DOI":"10.1021\/jf8003652","article-title":"Effect of Long-Term Dietary Administration of Oregano on the Alleviation of Carbon Tetrachloride-Induced Oxidative Stress in Rats","volume":"56","author":"Botsoglou","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"103209","DOI":"10.1016\/j.psj.2023.103209","article-title":"Circadian Regulation of Calcium and Phosphorus Homeostasis during the Oviposition Cycle in Laying Hens","volume":"103","author":"Blair","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_75","unstructured":"Magnuson, A.D. (2015). Novel Biomarkers for Calcium and Phosphorus Metabolism in Breeder Hens and Broilers. [Master\u2019s Thesis, University of Arkansas]."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1093\/jn\/117.3.619","article-title":"Calcium Logistics in the Laying Hen","volume":"117","author":"Etches","year":"1987","journal-title":"J. Nutr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"517","DOI":"10.4141\/cjas93-056","article-title":"Blood, Intestinal and Skeletal Calcium Dynamics during Egg Formation","volume":"73","author":"Clunies","year":"1993","journal-title":"Can. J. Anim. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1432-1033.1975.tb02176.x","article-title":"Calcitonin Biosynthesis: Evidence for a Precursor","volume":"55","author":"Moya","year":"1975","journal-title":"Eur. J. Biochem."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Tan, Y.B., and Langley, R.J. (2019). Sepsis: Metabolomics and Proteomics. Genomic and Precision Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-801496-7.00007-1"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"332","DOI":"10.7150\/ijms.39367","article-title":"Procalcitonin: A Promising Tool or Just Another Overhyped Test?","volume":"17","author":"Paudel","year":"2020","journal-title":"Int. J. Med. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Li, D., Zhong, C., Sun, Y., Kang, L., and Jiang, Y. (2023). Identification of Genes Involved in Chicken Follicle Selection by ONT Sequencing on Granulosa Cells. Front. Genet., 13.","DOI":"10.3389\/fgene.2022.1090603"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Zhao, J., Pan, H., Liu, Y., He, Y., Shi, H., and Ge, C. (2023). Interacting Networks of the Hypothalamic\u2013Pituitary\u2013Ovarian Axis Regulate Layer Hens Performance. Genes, 14.","DOI":"10.3390\/genes14010141"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3409\/fb60_3-4.213-217","article-title":"Independent, Non-IGF-I Mediated, GH Action on Estradiol Secretion by Prehierarchical Ovarian Follicles in Chicken. In Vitro Study","volume":"60","author":"Hrabia","year":"2012","journal-title":"Folia Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"103414","DOI":"10.1016\/j.psj.2023.103414","article-title":"Effects of Exogenous Energy on Synthesis of Steroid Hormones and Expression Characteristics of the CREB\/StAR Signaling Pathway in Theca Cells of Laying Hen","volume":"103","author":"Lu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"6997","DOI":"10.1021\/jf071085r","article-title":"Effect of Dietary Daidzein on Egg Production, Shell Quality, and Gene Expression of ER-\u03b1, GH-R, and IGF-IR in Shell Glands of Laying Hens","volume":"55","author":"Ni","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Hanlon, C., Ziezold, C.J., and B\u00e9d\u00e9carrats, G.Y. (2022). The Diverse Roles of 17\u03b2-Estradiol in Non-Gonadal Tissues and Its Consequential Impact on Reproduction in Laying and Broiler Breeder Hens. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.942790"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez-Grijalva, E., Picos-Salas, M., Leyva-L\u00f3pez, N., Criollo-Mendoza, M., Vazquez-Olivo, G., and Heredia, J. (2017). Flavonoids and Phenolic Acids from Oregano: Occurrence, Biological Activity and Health Benefits. Plants, 7.","DOI":"10.3390\/plants7010002"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.tiv.2019.01.005","article-title":"The Origin of In-Vitro Estrogen-like Activity in Oregano Herb Extracts","volume":"56","author":"Wielogorska","year":"2019","journal-title":"Toxicol. Vitr."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3797\/scipharm.1007-15","article-title":"Phytoestrogen Biological Actions on Mammalian Reproductive System and Cancer Growth","volume":"79","author":"Zhao","year":"2011","journal-title":"Sci. Pharm."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Saleh, A.A., Hamed, S., Hassan, A.M., Amber, K., Awad, W., Alzawqari, M.H., and Shukry, M. (2021). Productive Performance, Ovarian Follicular Development, Lipid Peroxidation, Antioxidative Status, and Egg Quality in Laying Hens Fed Diets Supplemented with Salvia officinalis and Origanum majorana Powder Levels. Animals, 11.","DOI":"10.3390\/ani11123513"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/12\/1213\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T11:26:46Z","timestamp":1766057206000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/12\/12\/1213"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,18]]},"references-count":90,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["vetsci12121213"],"URL":"https:\/\/doi.org\/10.3390\/vetsci12121213","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,18]]}}}