{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T21:04:46Z","timestamp":1783112686129,"version":"3.54.6"},"reference-count":47,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University","award":["ZDSYS2023004"],"award-info":[{"award-number":["ZDSYS2023004"]}]},{"name":"Department of Science and Technology, Hunan Province","award":["2016NK2014"],"award-info":[{"award-number":["2016NK2014"]}]},{"name":"Department of Education, Hunan Province","award":["17B126"],"award-info":[{"award-number":["17B126"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Spirometra mansoni is a zoonotic parasite that inhabits the intestines of dogs and cats. The plerocercoids (spargana) parasitize several vertebrates, including humans, resulting in a food-borne zoonosis known as sparganosis. In this study, it has been established that a LAMP assay can detect S. mansoni eggs in dog feces. A total of 97 fecal samples were collected from Changsha City, Hunan Province. The fecal DNA was extracted before designing primers for LAMP based on the S. mansoni cox1 gene. The specificity of this method was verified by PCR using LAMP outer primers or inner primers and nested PCR with S. mansoni-specific cox1 primers. DNA samples from five control dog worms were analyzed using the LAMP assay to evaluate the specificity. The detection rate of LAMP for S. mansoni eggs was 70.21% in stray dogs. PCR and nested PCR produced specific bands on agarose gel electrophoresis consistent with the expected length. When the LAMP assay was conducted using S. mansoni-infected samples, negative samples, and genomic DNA from control worms, only the S. mansoni-infected samples showed a typical ladder pattern. The samples were stained with SYBR Green I, and only the S. mansoni-infected samples had a fluorescent signal. In addition, compared with PCR and microscope, LAMP method can detect eggs in the shortest infection days, and its detection rate was higher than that of PCR. These results suggest that the established LAMP method have many advantages in detecting Spirometra mansoni.<\/jats:p>","DOI":"10.3390\/vetsci13010066","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:35:52Z","timestamp":1767954952000},"page":"66","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Spirometra mansoni in Dogs"],"prefix":"10.3390","volume":"13","author":[{"given":"Xiaoruo","family":"Tan","sequence":"first","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuke","family":"Zeng","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiquan","family":"Lu","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-7416","authenticated-orcid":false,"given":"Asmaa M. I.","family":"Abuzeid","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Meng","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Zou","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewei","family":"Fan","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-0397","authenticated-orcid":false,"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ijid.2016.10.013","article-title":"Prevalence of Spirometra mansoni in dogs, cats, and frogs and its medical relevance in Guangzhou, China","volume":"53","author":"Hong","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e273","DOI":"10.3346\/jkms.2018.33.e273","article-title":"Human sparganosis in Korea","volume":"33","author":"Kim","year":"2018","journal-title":"J. Korean Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3347\/kjp.1984.22.1.66","article-title":"Two cases of human infection by adult of Spirometra erinacei","volume":"22","author":"Lee","year":"1984","journal-title":"Korean J. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s40249-017-0265-7","article-title":"A neglected risk for sparganosis: Eating live tadpoles in central China","volume":"6","author":"Cui","year":"2017","journal-title":"Infect. Dis. Poverty"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/S1473-3099(15)00133-4","article-title":"Human sparganosis, a neglected food-borne zoonosis","volume":"15","author":"Liu","year":"2015","journal-title":"Lancet Infect. Dis."},{"key":"ref_6","first-page":"251","article-title":"Human plerocercoidosis and sparganosis: II. A historical review on pathology, clinics, epidemiology and control","volume":"27","author":"Qiu","year":"2009","journal-title":"Chin. J. Parasitol. Parasit. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1017\/S0950268814000144","article-title":"Retrospective epidemiological analysis of sparganosis in mainland China from 1959 to 2012","volume":"142","author":"Lu","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gong, T., Su, X., Li, F., He, J., Chen, S., Li, W., Xie, X., Liu, Y., Zhang, X., and Liu, W. (2022). Epidemiology and Genetic Diversity of Spirometra Tapeworm Isolates from Snakes in Hunan Province, China. Animals, 12.","DOI":"10.3390\/ani12091216"},{"key":"ref_9","unstructured":"Feiyang, L. (2020). Multi-source review on domestic stray-animal problems. 2020 2nd Scientific Workshop on Advanced in Social Sciences, Arts & Humanities, Francis Academic Press."},{"key":"ref_10","first-page":"101030","article-title":"Molecular, morphological and histopathological evidence of Spirometra mansoni in wild and domestic animals from Costa Rica","volume":"51","author":"Bass","year":"2024","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_11","first-page":"101189","article-title":"First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico","volume":"57","author":"Rojas","year":"2025","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.parint.2011.01.002","article-title":"Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera","volume":"60","author":"Rahman","year":"2011","journal-title":"Parasitol. Int."},{"key":"ref_13","first-page":"319","article-title":"Rapid detection of specific IgG in sera of patients with infection of Sparganum mansoni by dot immuno-gold filtration assay","volume":"24","author":"Wang","year":"2008","journal-title":"Chin. J. Zoonoses"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1017\/S003118202500054X","article-title":"Molecular characterization of Spirometra isolates across the USA","volume":"152","author":"Sanders","year":"2025","journal-title":"Parasitology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1186\/s13071-015-1183-9","article-title":"Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples","volume":"8","author":"Mugambi","year":"2015","journal-title":"Parasit Vectors"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/JCM.01573-08","article-title":"Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species","volume":"47","author":"Nkouawa","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic. Acids. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/bbrc.2001.5921","article-title":"Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation","volume":"289","author":"Mori","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.parint.2011.08.009","article-title":"Rapid detection of Opisthorchis viverrini copro-DNA using loop-mediated isothermal amplification (LAMP)","volume":"61","author":"Arimatsu","year":"2012","journal-title":"Parasitol. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.14411\/fp.2012.004","article-title":"Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)","volume":"59","author":"Hu","year":"2012","journal-title":"Folia Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"578","DOI":"10.4269\/ajtmh.2009.09-0145","article-title":"Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification","volume":"81","author":"Takagi","year":"2009","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.4269\/ajtmh.13-0583","article-title":"A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye","volume":"90","author":"Watts","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Bernalt Diego, J., Fern\u00e1ndez-Soto, P., Febrer-Sendra, B., Crego-Vicente, B., and Muro, A. (2021). Loop-mediated isothermal amplification in schistosomiasis. J. Clin. Med., 10.","DOI":"10.3390\/jcm10030511"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.micpath.2017.03.016","article-title":"Loop-mediated isothermal amplification (LAMP): A novel rapid detection platform for pathogens","volume":"107","author":"Li","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e00291","DOI":"10.1016\/j.fawpar.2025.e00291","article-title":"Development of PCR, qPCR and LAMP methods for the detection of Spirometra mansoni (Cestoda: Diphyllobothriidae) in the faeces of dogs and cats","volume":"41","author":"Li","year":"2025","journal-title":"Food Waterborne Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"309","DOI":"10.3347\/kjp.2019.57.3.309","article-title":"Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China","volume":"57","author":"He","year":"2019","journal-title":"Korean J. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.femsim.2004.08.005","article-title":"Detection of periodontal pathogen Porphyromonas gingivalis by loop-mediated isothermal amplification method","volume":"43","author":"Maeda","year":"2005","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.vetpar.2004.09.018","article-title":"Waterborne zoonotic helminthiases","volume":"126","author":"Nithiuthai","year":"2004","journal-title":"Vet. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.pt.2007.12.003","article-title":"Trends in parasitic diseases in the Republic of Korea","volume":"24","author":"Shin","year":"2008","journal-title":"Trends Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.ijid.2004.08.003","article-title":"A review of human sparganosis in Thailand","volume":"9","author":"Wiwanitkit","year":"2005","journal-title":"Int. J. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e154","DOI":"10.1016\/j.ijid.2010.10.001","article-title":"Sparganosis in mainland China","volume":"15","author":"Li","year":"2011","journal-title":"Int. J. Infect. Dis."},{"key":"ref_32","first-page":"636","article-title":"Prevalence of Spirometra mansoni infections in hosts in Jiangsu Province","volume":"33","author":"Tong","year":"2021","journal-title":"Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi"},{"key":"ref_33","first-page":"132","article-title":"Epidemiological investigation on sparganosis mansoni and animal experiments","volume":"28","author":"Lin","year":"2010","journal-title":"Chin. J. Parasitol. Parasit. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Foo, P.C., Nurul Najian, A.B., Muhamad, N.A., Ahamad, M., Mohamed, M., Yean Yean, C., and Lim, B.H. (2020). Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: A comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample. BMC Biotechnol., 20.","DOI":"10.1186\/s12896-020-00629-8"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rahman, S.M.M., Song, H.B., Jin, Y., Oh, J.-K., Lim, M.K., Hong, S.-T., and Choi, M.-H. (2017). Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005995"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"116808","DOI":"10.1016\/j.diagmicrobio.2025.116808","article-title":"Evaluation of loop mediated isothermal amplification, quantitative real-time PCR, conventional PCR methods for identifying Ascaris lumbricoides in human stool samples","volume":"112","author":"Thakur","year":"2025","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"110132","DOI":"10.1016\/j.vetpar.2024.110132","article-title":"Evaluation of LAMP for Fasciola hepatica detection from faecal samples of experimentally and naturally infected cattle","volume":"327","author":"Bari","year":"2024","journal-title":"Vet. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1007\/s00436-010-2206-2","article-title":"Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana","volume":"108","author":"Cui","year":"2011","journal-title":"Parasitol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/pim.12151","article-title":"The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay","volume":"37","author":"Chen","year":"2015","journal-title":"Parasite Immunol."},{"key":"ref_40","first-page":"121","article-title":"Evaluation of dot immunogold filtration assay (DIGFA) for rapid serodiagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea)","volume":"32","author":"Eamsobhana","year":"2015","journal-title":"Trop. Biomed."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pi-Bansa, S., Osei, J.H.N., Kartey-Attipoe, W.D., Elhassan, E., Agyemang, D., Otoo, S., Dadzie, S.K., Appawu, M.A., Wilson, M.D., and Koudou, B.G. (2019). Assessing the presence of Wuchereria bancrofti infections in vectors using xenomonitoring in lymphatic filariasis endemic districts in Ghana. Trop. Med. Infect. Dis., 4.","DOI":"10.3390\/tropicalmed4010049"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.parint.2011.08.018","article-title":"Development of loop-mediated isothermal amplification method for detecting Wuchereria bancrofti DNA in human blood and vector mosquitoes","volume":"60","author":"Takagi","year":"2011","journal-title":"Parasitol. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"109885","DOI":"10.1016\/j.vetpar.2023.109885","article-title":"Comparison of multiplex copro PCR with coproscopy followed by PCR on recovered eggs for the detection of Echinococcus granulosus and Taenia spp. infection in dogs","volume":"315","author":"Genad","year":"2023","journal-title":"Vet. Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110073","DOI":"10.1016\/j.vetpar.2023.110073","article-title":"Diagnosis of canine intestinal parasites: Improved detection of Dipylidium caninum infection through coproantigen testing","volume":"324","author":"Little","year":"2023","journal-title":"Vet. Parasitol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Tylkowska, A., Mocha, N., Ko\u0142nierzak, M.M., and Szenejko, M. (2024). Risk factors associated with soil-transmitted helminths in dog feces that contaminate public areas of Warsaw, Poland. Animals, 14.","DOI":"10.3390\/ani14030450"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1016\/j.ijpara.2005.06.010","article-title":"Schistosomiasis Transmission And Ecology In The Philippines Step Project. Estimating sensitivity and specificity of a faecal examination method for Schistosoma japonicum infection in cats, dogs, water buffaloes, pigs, and rats in Western Samar and Sorsogon Provinces, The Philippines","volume":"35","author":"Carabin","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/trstmh\/trv100","article-title":"Current status of Clonorchis sinensis and clonorchiasis in China","volume":"110","author":"Lai","year":"2016","journal-title":"Trans. R. Soc. Trop. Med. Hyg."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/1\/66\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:51:29Z","timestamp":1767955889000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/1\/66"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":47,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["vetsci13010066"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13010066","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]}}}