{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T12:06:54Z","timestamp":1772712414399,"version":"3.50.1"},"reference-count":70,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Siirt University Scientific Research Projects Coordination Office","award":["2024-SIUVET-018"],"award-info":[{"award-number":["2024-SIUVET-018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study investigated the effects of different PRP doses and application strategies on sperm quality in goats during cryopreservation. Semen samples were collected weekly from six Boer goats and treated with PRP either by means of pre-incubation or supplementation of the semen extender at concentrations of 10, 20, and 40 \u00d7 106 platelets\/mL. Computer-assisted sperm analysis (CASA) was performed after equilibration and again after thawing to assess sperm motility parameters, including progressive motility. Post-thaw sperm functional integrity was further evaluated using flow cytometry, including assessments of plasma membrane integrity, acrosome integrity, high mitochondrial membrane potential (hMMP), sperm viability, oxidative stress, and DNA integrity. The results demonstrated that pre-incubation with PRP at 10 \u00d7 106 platelets\/mL (pre-PRP10) yielded the most pronounced improvements in total and progressive motility after thawing, as well as in plasma membrane integrity, viability, and mitochondrial activity, while significantly reducing oxidative stress and DNA fragmentation compared with other PRP doses and application strategies. Supplementation of the extender with PRP at 20 \u00d7 106 platelets\/mL also showed favorable effects on functional sperm parameters; however, it did not result in significant improvements in oxidative stress or DNA fragmentation. In contrast, the highest PRP dose (40 \u00d7 106 platelets\/mL) did not confer additional benefits. None of the PRP treatments improved post-thaw acrosome integrity. In conclusion, these findings highlight the importance of PRP dose optimization and direct sperm\u2013PRP interaction, identifying pre-incubation with PRP at 10 \u00d7 106 platelets\/mL as the most effective approach for enhancing post-thaw sperm quality in goats.<\/jats:p>","DOI":"10.3390\/vetsci13030245","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T10:31:14Z","timestamp":1772706674000},"page":"245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Platelet-Rich Plasma Dose and Application Strategy on Post-Thaw Spermatological Parameters in Goat Semen"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1326-2678","authenticated-orcid":false,"given":"Ahmet","family":"Eser","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt TR-56100, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0535-4089","authenticated-orcid":false,"given":"Kemal","family":"Ba\u011fc\u0131","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt TR-56100, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdurrahman","family":"Alaku\u015f","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Institute of Health Sciences, Dicle University, Diyarbak\u0131r TR-21280, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asl\u0131han \u00c7ak\u0131r","family":"Cihangiro\u011flu","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt TR-56100, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130kra","family":"Karaa\u011fa\u00e7","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Graduate Education Institute, Istanbul University-Cerrahpa\u015fa, Istanbul TR-34320, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selin","family":"Ya\u011fc\u0131o\u011flu","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul TR-34320, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2236-2526","authenticated-orcid":false,"given":"Ramazan","family":"Ar\u0131c\u0131","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul TR-34320, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4561-6189","authenticated-orcid":false,"given":"Kamber","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul TR-34320, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.cryobiol.2017.06.008","article-title":"Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation","volume":"78","author":"Medrano","year":"2017","journal-title":"Cryobiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.theriogenology.2015.09.047","article-title":"Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs","volume":"85","author":"Yeste","year":"2016","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.rbmo.2018.05.012","article-title":"Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches","volume":"37","author":"Hezavehei","year":"2018","journal-title":"Reprod. 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