{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:01:43Z","timestamp":1785420103156,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Debrecen Program for Scientific Publication"},{"id":[{"id":"https:\/\/ror.org\/02xf66n48","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The effects of reproductive technologies, namely, artificial insemination (AI) and multiple ovulation embryo transfer (MOET), on the production, health, and longevity of Holstein\u2013Friesian cows were evaluated. Data were obtained from the time period between 2017 and 2024 on a Hungarian dairy farm and consisted of 1783 cows (1544 AI and 239 MOET). Deep-frozen semen from identical bulls was used for both the AI and MOET groups. Disease incidence, productive life, and early-lactation milk production phenotypes were collected in these cows. MOET cows demonstrated significantly higher milk yield during the first 100 days of lactation but had a 43.9% greater risk of culling compared with AI cows (p &lt; 0.05). Metabolic and reproductive disorders were the most common reasons for culling cows, with increased frequency of health issues correlating with higher culling risk ratios (p &lt; 0.05). While MOET cows showed lower incidences of metabolic disorders, reproductive problems, and mastitis, their shorter productive lifespan likely limited overall disease exposure. Nevertheless, when MOET cows experienced illness, the impact was more severe, particularly in relation to metabolic issues (p &lt; 0.05). These findings highlight trade-offs between improved genetic potential and health resilience in MOET-derived cows. Despite their higher productivity, their management may require greater health vigilance. This study offers practical insights for dairy producers in selecting reproductive strategies to balance genetic gain, herd health, and longevity under intensive production systems.<\/jats:p>","DOI":"10.3390\/vetsci13040320","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T15:10:24Z","timestamp":1774537824000},"page":"320","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effect of Artificial Insemination and Multiple Ovulation Embryo Transfer on Production, Health Status, and Survival of Holstein\u2013Friesian Cows"],"prefix":"10.3390","volume":"13","author":[{"given":"Eszter","family":"T\u00f3th","sequence":"first","affiliation":[{"name":"Doctoral School of Animal Science, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7208-1896","authenticated-orcid":false,"given":"J\u00e1nos","family":"Posta","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Istv\u00e1n","family":"Koml\u00f3si","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3763-857X","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"Szel\u00e9nyi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Farm Animal Medicine Clinic, University of Veterinary Science, Istv\u00e1n Street 2, 1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Gonda","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Animal Science Complex, Box 2170, Brookings, SD 57007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"R\u00e1tky","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Farm Animal Medicine Clinic, University of Veterinary Science, Istv\u00e1n Street 2, 1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4863-8287","authenticated-orcid":false,"given":"Ren\u00e1ta","family":"Knop","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.3168\/jds.S0022-0302(06)72257-3","article-title":"Invited Review: Culling: Nomenclature, definitions, and recommendations","volume":"89","author":"Fetrow","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"387","DOI":"10.5194\/aab-59-387-2016","article-title":"Genetic analysis of productive life length in Holstein dairy cows using Weibull proportional risk model","volume":"59","author":"Najafabadi","year":"2016","journal-title":"Arch. Anim. Breed."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.17221\/3946-CJAS","article-title":"Relationships between conformation traits and longevity of Holstein cows in the Czech Republic","volume":"51","author":"Vacek","year":"2018","journal-title":"Czech J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"90","DOI":"10.31073\/abg.61.11","article-title":"The influence of genotypic factors on longevity and lifetime production of dairy cows","volume":"61","author":"Polupan","year":"2021","journal-title":"Anim. Breed. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100066","DOI":"10.1016\/j.anopes.2024.100066","article-title":"Exploring critical animal-based traits as potential predictors of production diseases in dairy cattle: A systematic review and meta-analysis","volume":"3","author":"Alrhmoun","year":"2024","journal-title":"Anim. Open Space"},{"key":"ref_6","first-page":"1590","article-title":"Association between body energy content in the dry period and post-calving production disease status in dairy cattle","volume":"11","author":"Smith","year":"2017","journal-title":"Animals"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Grzesiak, W., Adamczyk, K., Zaborski, D., and W\u00f3jcik, J. (2022). Estimation of Dairy Cow Survival in the First Three Lactations for Different Culling Reasons Using the Kaplan\u2013Meier Method. Animals, 12.","DOI":"10.3390\/ani12151942"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.5433\/1679-0359.2021v42n6p3449","article-title":"Relationship between metabolic profile, diseases, productive and reproductive performance in high- producing Holstein cows in the postpartum period","volume":"42","author":"Askel","year":"2021","journal-title":"Semin. Ci\u00eancias Agr\u00e1rias"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4575","DOI":"10.3168\/jds.2020-18792","article-title":"Prepartum behavior changes in dry Holstein cows at risk of postpartum diseases","volume":"104","author":"Belaid","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.3168\/jds.2016-11767","article-title":"Phenotypic, genetic, and single nucleotide polymorphism marker associations between calf diseases and subsequent performance and disease occurrences of first-lactation German Holstein cows","volume":"100","author":"Mahmoud","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_11","first-page":"881","article-title":"Multiple Ovulation and Embryo Transfer","volume":"3","author":"Lonergan","year":"2020","journal-title":"Encycl. Dairy Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1111\/rda.14417","article-title":"Monitoring of health status, performance and transcript abundance of some genes in dairy heifers produced by embryo transfer or artificial insemination","volume":"58","author":"Hussein","year":"2023","journal-title":"Reprod. Domest. Anim. Zuchthyg."},{"key":"ref_13","first-page":"562","article-title":"A Review of Embryo Transfer Technology in Cattle","volume":"3","author":"Weldegebriel","year":"2015","journal-title":"Glob. J. Anim. Sci. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1071\/RD20207","article-title":"Emerging diseases in international trade in embryos","volume":"33","author":"Schnuelle","year":"2021","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.3389\/fgene.2019.01197","article-title":"A Vision for Development and Utilization of High-Throughput Phenotyping and Big Data Analytics in Livestock","volume":"10","author":"Koltes","year":"2019","journal-title":"Front. Genet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/af\/vfaa007","article-title":"The future of phenomics in dairy cattle breeding","volume":"10","author":"Cole","year":"2020","journal-title":"Anim. Front. Rev. Mag. Anim. Agric."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.theriogenology.2011.09.002","article-title":"Impact of genomic selection of AI dairy sires on their likely utilization and methods to estimate fertility: A paradigm shift","volume":"77","author":"Amann","year":"2012","journal-title":"Theriogenology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4414","DOI":"10.3168\/jds.2007-0980","article-title":"Efficient methods to compute genomic predictions","volume":"91","author":"VanRaden","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_19","first-page":"541384","article-title":"Evaluation of bovine embryo biopsy techniques according to their ability to preserve embryo viability","volume":"1","author":"Cenariu","year":"2012","journal-title":"BioMed Res. Int."},{"key":"ref_20","first-page":"61","article-title":"Recuperaci\u00f3n y transferencia de embriones de vacas Holstein de elite con infecciones uterinas recurrentes","volume":"11","author":"Velez","year":"1998","journal-title":"Rev. Colomb. Cienc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4380","DOI":"10.3168\/jds.2022-22736","article-title":"Comparison of cattle derived from in vitro fertilization, multiple ovulation embryo transfer, and artificial insemination for milk production and fertility traits","volume":"106","author":"Lafontaine","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.cmpb.2013.01.010","article-title":"The Survival Kit: Software to analyze survival data including possibly correlated random effects","volume":"110","author":"Ducrocq","year":"2013","journal-title":"Comput. Methods Programs Biomed."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1017\/S002202991200060X","article-title":"Predisposing factors for involuntary culling in Holstein\u2013Friesian dairy cows","volume":"80","author":"Chiumia","year":"2013","journal-title":"J. Dairy Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1136\/vr.140.2.36","article-title":"Culling in 50 dairy herds in England","volume":"140","author":"Esslemont","year":"1997","journal-title":"Vet. Rec."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.3168\/jds.2017-13439","article-title":"The contribution of previous lameness events and body condition score to the occurrence of lameness in dairy herds: A study of 2 herds","volume":"101","author":"Randall","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.anifeedsci.2005.08.003","article-title":"Feeding- and management-related diseases in the transition cow: Physiological adaptations around calving and strategies to reduce feeding-related diseases","volume":"126","author":"Ingvartsen","year":"2006","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9419","DOI":"10.3168\/jds.2018-14422","article-title":"Association between metabolic diseases and the culling risk of high-yielding dairy cows in a transition management facility using survival and decision tree analysis","volume":"101","author":"Probo","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rilanto, T., Reimus, K., Orro, T., Emanuelson, U., Viltrop, A., and M\u00f5tus, K. (2020). Dairy Cow Culling\u2014Reasons and Risk Factors. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02384-6"},{"key":"ref_29","first-page":"1843","article-title":"Reproductive problems and welfare of dairy cows","volume":"70","year":"2013","journal-title":"Vet. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1051\/vetres:2003020","article-title":"Genetics of resistance to mastitis in dairy cattle","volume":"34","author":"Rupp","year":"2003","journal-title":"Vet. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4117","DOI":"10.3168\/jds.2014-9090","article-title":"Genetic analysis of longevity in Dutch dairy cattle using random regression","volume":"98","author":"Meuwissen","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1111\/rda.14409","article-title":"Comparative productive performance of cows born through embryo transfer, artificial insemination and natural mating in dairy and dual-purpose herds raised in tropical conditions","volume":"58","author":"Galina","year":"2023","journal-title":"Reprod. Domest. Anim."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.crvi.2010.07.001","article-title":"Evaluation of the genetic trend of milk yield in the multiple ovulation and embryo transfer populations of dairy cows, using stochastic simulation","volume":"333","year":"2010","journal-title":"Comptes Rendus Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3168\/jds.2013-6943","article-title":"Consequences of transfer of an in vitro-produced embryo for the dam and resultant calf","volume":"97","author":"Bonilla","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5913","DOI":"10.3168\/jds.2023-24051","article-title":"A large population study to assess the magnitude of prenatal programming in dairy cattle","volume":"107","author":"Sanchez","year":"2024","journal-title":"J. Dairy Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/320\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:13:17Z","timestamp":1775059997000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,26]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["vetsci13040320"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13040320","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,26]]}}}