{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:27:15Z","timestamp":1774621635310,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Veterinary Medicine\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t              https:\/\/ror.org\/03vayv672","award":["SRF-003"],"award-info":[{"award-number":["SRF-003"]}]},{"name":"University of Veterinary Medicine\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t              https:\/\/ror.org\/03vayv672","award":["SRF-002"],"award-info":[{"award-number":["SRF-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The recovery and cryopreservation of epididymal spermatozoa enable genetic preservation in male dogs that die unexpectedly or require castration; however, sperm collection is typically performed immediately after surgery, and artificial insemination is often surgical. This study aimed to evaluate whether epididymides stored at 4\u20135 \u00b0C for 24 h prior to sperm recovery retain fertilizing capacity after cryopreservation and whether pregnancy can be achieved using endoscopically guided transcervical intrauterine insemination. Testes and epididymides from an eight-month-old German Shepherd dog were stored in physiological saline at 4\u20135 \u00b0C for 24 h following castration. Spermatozoa were recovered from the cauda epididymis using single-incision aspiration, evaluated, frozen according to the Uppsala method, and stored in liquid nitrogen for two months. After thawing, 62 \u00d7 106 progressive motile spermatozoa were inseminated once into a bitch in heat using transcervical endoscopic guidance. Pregnancy was confirmed by ultrasonography, and nine healthy puppies were delivered at term. These findings demonstrate that 24 h refrigerated storage of the epididymis does not impair post-thaw fertilizing ability and that non-surgical transcervical intrauterine insemination represents an effective alternative to surgical techniques for the use of frozen\u2013thawed epididymal semen in dogs.<\/jats:p>","DOI":"10.3390\/vetsci13040326","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:45:53Z","timestamp":1774615553000},"page":"326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Successful Endoscope-Assisted Transcervical Insemination (TCI) in Dogs Using Sperm Recovered from Epididymides Stored at 5 \u00b0C for 24 h After Castration Prior to Semen Collection and Cryopreservation"],"prefix":"10.3390","volume":"13","author":[{"given":"M\u00f3nika","family":"Bacsa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"Szil\u00e1gyi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristi\u00e1n","family":"Erdei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bal\u00e1zs Attila","family":"Dobos","sequence":"additional","affiliation":[{"name":"Heart and Vascular Center, Faculty of Medicine, Semmelweis University, 1122 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Radovits","sequence":"additional","affiliation":[{"name":"Heart and Vascular Center, Faculty of Medicine, Semmelweis University, 1122 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8251-4712","authenticated-orcid":false,"given":"S\u00e1ndor","family":"Cseh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.6061\/clinics\/2013(Sup01)15","article-title":"Cryopreservation of testicular and epididymal sperm: Techniques and clinical outcomes of assisted conception","volume":"68","author":"Gangrade","year":"2013","journal-title":"Clinics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1111\/rda.14171","article-title":"Effects of epididymal sperm recovery methods on fresh and frozen\u2013thawed sperm characteristics in dogs","volume":"57","author":"Mogheiseh","year":"2022","journal-title":"Reprod. 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