{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:27:30Z","timestamp":1785403650431,"version":"3.56.0"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Posgrado Conjunto Agronom\u00eda\u2013Veterinaria"},{"name":"Faculty of Veterinary Medicine and Zootechnics, Universidad Aut\u00f3noma de Nuevo Le\u00f3n"},{"id":[{"id":"https:\/\/ror.org\/01fh86n78","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Endogenous heat shock cognate 73 kDa protein (HSC70) plays a role in early embryonic development and cellular stress regulation. This study evaluated the effects of exogenous recombinant HSC70 supplementation on bovine embryo development and the expression of apoptosis-related genes under in vitro conditions. Expression analyses of HSPA1A, HSPA8, BCL-2, and BAX were performed on Day 7 bovine embryos produced in vivo and in vitro. In vitro embryos exhibited higher basal expressions of HSPA8, BAX and BCL-2 compared with in vivo embryos (p \u2264 0.001). Supplementation with 500 or 1000 ng\/mL HSC70 was associated with increased expression of HSPA1A, HSPA8, BCL-2, and BAX relative to control embryos (p \u2264 0.01). The 1000 ng\/mL group showed significantly higher HSPA8 expression compared with both the control and 500 ng\/mL groups. Morphological evaluation indicated that embryos supplemented with 500 ng\/mL were associated with improved blastocyst yield and quality compared with control and 1000 ng\/mL groups.In conclusion, supplementation with 500 ng\/mL recombinant HSC70 was associated with modulation of apoptosis-related gene expression and improved morphological developmental parameters under in vitro conditions. These findings indicate dose-dependent regulatory effects of HSC70 on apoptosis-related signaling pathways; however, as apoptosis was assessed at the transcriptional level, the results should be interpreted as molecular associations rather than direct confirmation of altered apoptotic activity.<\/jats:p>","DOI":"10.3390\/vetsci13040339","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:34:08Z","timestamp":1774974848000},"page":"339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dose-Dependent Effects of Heat Shock Cognate 70 on Viability and Apoptosis-Related Gene Expression in In Vitro-Produced Bovine Embryos"],"prefix":"10.3390","volume":"13","author":[{"given":"Aim\u00e9 Jazmin","family":"Garza-Arredondo","sequence":"first","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Nutrici\u00f3n y Producci\u00f3n Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, General Escobedo 66054, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2225-0495","authenticated-orcid":false,"given":"Diana Eliza","family":"Zamora-\u00c1vila","sequence":"additional","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Nutrici\u00f3n y Producci\u00f3n Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, General Escobedo 66054, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Moreno-Degollado","sequence":"additional","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Nutrici\u00f3n y Producci\u00f3n Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, General Escobedo 66054, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisse Melisa","family":"Garza Hernandez","sequence":"additional","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Nutrici\u00f3n y Producci\u00f3n Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, General Escobedo 66054, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Fernando","family":"de la Torre Sanchez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Tepatitl\u00e1n de Morelos, Jalisco 47600, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"P\u00e9rez-Reynoso","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Tepatitl\u00e1n de Morelos, Jalisco 47600, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-725X","authenticated-orcid":false,"given":"Rub\u00e9n","family":"Cervantes-Vega","sequence":"additional","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Nutrici\u00f3n y Producci\u00f3n Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, General Escobedo 66054, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0342-1703","authenticated-orcid":false,"given":"Uziel","family":"Castillo-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Campus de Ciencias Agropecuarias, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Ave. Francisco Villa s\/n, Ex-Hacienda El Canad\u00e1, General Escobedo 66050, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1095\/biolreprod66.3.589","article-title":"Analysis of differential messenger RNA expression between bovine blastocysts produced in different culture systems: Implications for blastocyst quality","volume":"66","author":"Rizos","year":"2002","journal-title":"Biol. 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