{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:41:31Z","timestamp":1785498091876,"version":"3.56.0"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Background: Feline chronic gingivostomatitis (FCGS) is refractory stomatitis in cats. The cure rate of tooth extraction selected as a primary surgical treatment is insufficient. Methods: 52 FCGS-suspected cats, including 22 tooth-extracted and 30 unextracted cats, were studied. Commercially available antiviral and antibacterial agents were orally administered as initial treatment, followed by the antiviral agent solely as maintenance therapy. We examined the influence of prior tooth extraction on the therapeutic efficacy of these two drugs by analyzing changes in some physical signs and clinical laboratory biomarkers. Results: Mass spectrometric analysis revealed the active ingredients of antibacterial and antiviral were Moxifloxacin and Molnupiravir, respectively. All physical signs (weight, appetite, activity level, grooming behavior, ptyalism, erythema) showed statistically significant improvements with combined administration of these drugs. Numbers of white blood cells, neutrophils and monocytes, as well as circulating levels of total protein, albumin, globulin, and serum amyloid-A all statistically significantly decreased with their administration (p &lt; 0.0001). Conclusions: No statistically significant differences were detected between two FCGS groups in the changes in any of the above physical signs or clinical biomarker levels, indicating combination therapy with antibacterial and antiviral agents leads to effective treatment of FCGS, regardless of whether prior tooth extraction was performed or not.<\/jats:p>","DOI":"10.3390\/vetsci13040363","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:49:44Z","timestamp":1775641784000},"page":"363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combination Therapeutic Effect of Antibacterial and Antiviral Agents on Feline Chronic Gingivostomatitis Nonbounded to Prior Tooth Extraction Confirmed by Physical Signs and Clinical Biomarkers"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2973-2988","authenticated-orcid":false,"given":"Masato","family":"Katayama","sequence":"first","affiliation":[{"name":"Bloom Animal Hospital, Kajiyama 1-10-32, Tsurumi, Yokohama City 230-0072, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukina","family":"Uemura","sequence":"additional","affiliation":[{"name":"Bloom Animal Hospital, Kajiyama 1-10-32, Tsurumi, Yokohama City 230-0072, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.jfms.2007.03.003","article-title":"Prevalence of feline chronic gingivo-stomatitis in first opinion veterinary practice","volume":"9","author":"Healey","year":"2007","journal-title":"J. Feline Med. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1098612X221131453","DOI":"10.1177\/1098612X221131453","article-title":"Prevalence of feline chronic gingivostomatitis in feral cats and its risk factors","volume":"25","author":"Kim","year":"2023","journal-title":"J. Feline Med. Surg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1098612X231186834","DOI":"10.1177\/1098612X231186834","article-title":"Feline chronic gingivostomatitis current concepts in clinical management","volume":"25","author":"Goldschmidt","year":"2023","journal-title":"J. Feline Med. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Druet, I., and Hennet, P. (2017). Relationship between Feline calicivirus Load, Oral Lesions, and Outcome in Feline Chronic Gingivostomatitis (Caudal Stomatitis): Retrospective Study in 104 Cats. Front. Vet. Sci., 4.","DOI":"10.3389\/fvets.2017.00209"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1098612X241310590","DOI":"10.1177\/1098612X241310590","article-title":"Feline chronic gingivostomatitis: A thorough systematic review of associated factors","volume":"27","year":"2025","journal-title":"J. Feline Med. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"654","DOI":"10.2460\/javma.246.6.654","article-title":"Effect of tooth extraction on stomatitis in cats: 95 cases (2000\u20132013)","volume":"246","author":"Jennings","year":"2015","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"381","DOI":"10.2460\/ajvr.82.5.381","article-title":"Use of unbiased metagenomic and transcriptomic analyses to investigate the association between feline calicivirus and feline chronic gingivostomatitis in domestic cats","volume":"82","author":"Fried","year":"2021","journal-title":"Am. J. Vet. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1177\/1098612X18761274","article-title":"Prevalence of microorganisms associated with feline gingivostomatitis","volume":"21","author":"Nakanishi","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hofmann-Lehmann, R., Hosie, M.J., Hartmann, K., Egberink, H., Truyen, U., Tasker, S., Bel\u00e1k, S., Boucraut-Baralon, C., Frymus, T., and Lloret, A. (2022). Calicivirus Infection in Cats. Viruses, 14.","DOI":"10.3390\/v14050937"},{"key":"ref_10","unstructured":"Wogan, L. (2026, February 01). Unproven Treatment for Oral Disease in Cats Surfaces in US. Available online: https:\/\/news.vin.com\/default.aspx?pid=210&Id=12099794&f5=1#update."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1098612X2513398793","DOI":"10.1177\/1098612X251398793","article-title":"2025 FelineVMA feline oral health and dental care guidelines","volume":"27","author":"Lobprise","year":"2025","journal-title":"J. Feline Med. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1177\/1098612X16628578","article-title":"Clinical, pathological, immunohistochemical and molecular characterization of feline chronic gingivostomatitis","volume":"19","author":"Rolim","year":"2017","journal-title":"J. Feline Med. Surg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1177\/089875641303000101","article-title":"Efficacy of cyclosporine for chronic, refractory stomatitis in cats: A randomized, placebo-controlled, double-blinded clinical study","volume":"30","author":"Lommer","year":"2013","journal-title":"J. Vet. Dent."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.jfms.2011.05.012","article-title":"Comparative efficacy of a recombinant feline interferon omega in refractory cases of calicivirus-positive cats with caudal stomatitis: A randomised, multi-centre, controlled, double-blind study in 39 cats","volume":"13","author":"Hennet","year":"2011","journal-title":"J. Feline Med. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.rvsc.2018.10.003","article-title":"Evaluation of the efficacy of the subcutaneous low recombinant feline interferon-omega administration protocol for feline chronic gingivitis-stomatitis in feline calicivirus-positive cats","volume":"121","author":"Matsumoto","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Coelho, J.C., Duarte, N., Bento da Silva, A., Bronze, M.D.R., and Mestrinho, L.A. (2023). Placebo-Controlled Trial of Daily Oral Cannabidiol as Adjunctive Treatment for Cats with Chronic Gingivostomatitis. Animals, 13.","DOI":"10.3390\/ani13172716"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.14202\/vetworld.2025.2344-2356","article-title":"Efficacy and safety of omega-3-enriched lickable treats as adjunctive therapy for feline chronic gingivostomatitis: A randomized controlled trial","volume":"18","author":"Sukho","year":"2025","journal-title":"Vet. World"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Choe, K.H., Jang, K., Kim, S.E., and Jo, H.M. (2025). Long-term efficacy of cyclosporine and interferon-\u03c9 in feline chronic gingivostomatitis: Insights from SDAI scores. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-05141-9"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/S0924-8579(02)00190-5","article-title":"Antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections","volume":"20","author":"Milazzo","year":"2002","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_20","first-page":"929","article-title":"Moxifloxacin and its therapeutic uses in animals: An overview","volume":"7","author":"Meena","year":"2019","journal-title":"J. Entomol. Zool. Stud."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/s41586-021-03312-w","article-title":"SARS-CoV-2 infection is effectively treated and prevented by EIDD-2801","volume":"591","author":"Wahl","year":"2021","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1177\/1098612X211048647","article-title":"Investigation of monotherapy and combined anticoronaviral therapies against feline coronavirus serotype II in vitro","volume":"24","author":"Cook","year":"2022","journal-title":"J. Feline Med. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Katayama, M., Uemura, Y., and Katori, D. (2024). Effect of Nucleic Acid Analog Administration on Fluctuations in the Albumin-to-Globulin Ratio in Cats with Feline Infectious Peritonitis. Animals, 14.","DOI":"10.20944\/preprints202403.0709.v1"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Fumian, T.M., Tuipulotu, D.E., Netzler, N.E., Lun, J.H., Russo, A.G., Yan, G.J.H., and White, P.A. (2018). Potential Therapeutic Agents for Feline Calicivirus Infection. Viruses, 10.","DOI":"10.3390\/v10080433"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"105839","DOI":"10.1016\/j.antiviral.2024.105839","article-title":"Molnupiravir inhibits human norovirus and rotavirus replication in 3D human intestinal enteroids","volume":"223","author":"Chiu","year":"2024","journal-title":"Antivir. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"113201","DOI":"10.1016\/j.ejmech.2021.113201","article-title":"RNA-dependent RNA polymerase (RdRp) inhibitors: The current landscape and repurposing for the COVID-19 pandemic","volume":"213","author":"Tian","year":"2021","journal-title":"Eur. J. Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Winer, J.N., Arzi, B., and Verstraete, F.J. (2016). Therapeutic Management of Feline Chronic Gingivostomatitis: A Systematic Review of the Literature. Front. Vet. Sci., 3.","DOI":"10.3389\/fvets.2016.00054"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Vapniarsky, N., Simpson, D.L., Arzi, B., Taechangam, N., Walker, N.J., Garrity, C., Bulkeley, E., and Borjesson, D.L. (2020). Histological, Immunological, and Genetic Analysis of Feline Chronic Gingivostomatitis. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00310"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lopes, R.S., Carvalho, P.P., Pires, M.A., Rodrigues-Santos, P., Costa, E., and Requicha, J.F. (2025). Local and systemic immunological response in feline chronic gingivostomatitis: A critical review. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1572631"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e70525","DOI":"10.1002\/vms3.70525","article-title":"Assessment of Selected Endothelial Damage Biomarkers in the Determination of Endothelial Damage in Cats with Gingivostomatitis","volume":"11","author":"Korkmaz","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e70606","DOI":"10.1002\/vms3.70606","article-title":"Serum Amyloid A and Haptoglobin as Markers in Cats with Gingivitis-Preliminary Study","volume":"11","author":"Oikonomidis","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1136\/vr.152.5.125","article-title":"Salivary and serum immunoglobulin levels in cats with chronic gingivostomatitis","volume":"152","author":"Harley","year":"2003","journal-title":"Vet. Rec."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1002\/sctm.17-0035","article-title":"Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis","volume":"6","author":"Arzi","year":"2017","journal-title":"Stem Cells Transl. Med."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/363\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:40:29Z","timestamp":1775644829000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,8]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["vetsci13040363"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13040363","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,8]]}}}