{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T13:02:28Z","timestamp":1776430948704,"version":"3.51.2"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Pigeonpox is a significant infectious disease caused by Pigeonpox virus (PPV), which severely impacts the pigeon industry. Current control methods primarily rely on heterologous vaccines, such as those derived from avian poxviruses, but their protection is limited, creating an urgent need for the development of a specific vaccine. In this study, 720 samples collected from several regions of China between 2022 and 2023 were tested for PPV, followed by virus isolation, identification, and genetic evolutionary analysis. Based on these findings, complete genome sequencing and attenuation of the representative BJ-02 isolate were conducted, and the potential of this strain as a candidate for an attenuated vaccine was preliminarily evaluated. The survey showed PCR positive rates of 9.05%, 16.11%, and 12.50% in samples from Beijing, Guangdong, and Hainan, respectively. Six viral strains were isolated, all of which produced typical lesions on chorioallantoic membranes (CAM) and chicken embryo fibroblasts (CEF). Phylogenetic analysis based on the P4b gene revealed that the six viruses clustered within the same evolutionary branch, closely related to PPV and penguinpox virus strains from South Africa, India, and Taiwan, China. Complete genome sequencing of the BJ-02 strain showed its genomic structure to be similar to that of other fowlpox viruses, with some differences. After serial passage in CAM, PEF and CEF, the BJ-02 SD55 high-passage strain adapted well to in vitro culture, exhibited significantly reduced pathogenicity in chicken embryos and pigeons, and showed no reversion to virulence after five consecutive back-passages. Animal immunization tests demonstrated that the BJ-02 SD55 suspected attenuated strain induced specific antibodies and provided 100% protection against challenge with the virulent strain. In conclusion, PPV is widely prevalent in China. The BJ-02 strain, successfully isolated and attenuated through serial passage, demonstrates excellent immunogenicity and high safety, making it a promising candidate for a specific pigeonpox vaccine. Additionally, the complete genome characterization of BJ-02 contributes to the avian poxvirus genome database and provides critical data to support research on viral pathogenesis and the development of viral vector vaccines for avian and potentially mammalian species.<\/jats:p>","DOI":"10.3390\/vetsci13040393","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:53:46Z","timestamp":1776426826000},"page":"393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Complete Genome Sequencing of a Pigeonpox Virus Strain from Mainland China and Preliminary Evaluation of Its Attenuated Potential"],"prefix":"10.3390","volume":"13","author":[{"given":"Yifan","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baolichen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"},{"name":"Institute of Laboratory Animals Science, Peking Union Medical College (Chinese Academy of Medical Sciences), Beijing 100730, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongshu","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingliang","family":"Su","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-0897","authenticated-orcid":false,"given":"Jianyu","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1934-7111","authenticated-orcid":false,"given":"Kai","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.5455\/OVJ.2025.v15.i5.31","article-title":"Molecular characterization of fowl and pigeon pox viruses: A recent outbreak in Libya","volume":"15","author":"Elshwihdi","year":"2025","journal-title":"Open Vet. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s13567-014-0098-3","article-title":"Diversity of avipoxviruses in captive-bred Houbara bustard","volume":"45","author":"Ducatez","year":"2014","journal-title":"Vet. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"179","DOI":"10.30802\/AALAS-CM-18-000074","article-title":"Poxvirus Infection in a Colony of Laboratory Pigeons (Columba livia)","volume":"69","author":"Hibl","year":"2019","journal-title":"Comp. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chac\u00f3n, R.D., Astolfi-Ferreira, C.S., Valdeiglesias Ichillumpa, S., Lage Hagemann, H., Furlan Rocha, M., Fernandes Magalh\u00e3es, L., Freitas Raso, T., and Ferreira, A.J.P. (2025). First Complete Genome of Reticuloendotheliosis Virus in a Mallard Duck from Brazil: Phylogenetic Insights and Evolutionary Analysis. Pathogens, 14.","DOI":"10.3390\/pathogens14020189"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chac\u00f3n, R.D., Astolfi-Ferreira, C.S., Pereira, P.C., Assayag, M.S., Campos-Salazar, A.B., De la Torre, D., S\u00e1, L.R.M.d., Almeida, S.R.Y.d., Rici, R.E.G., and Ferreira, A.J.P. (2022). Outbreaks of Avipoxvirus Clade E in Vaccinated Broiler Breeders with Exacerbated Beak Injuries and Sex Differences in Severity. Viruses, 14.","DOI":"10.3390\/v14040773"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"001849","DOI":"10.1099\/jgv.0.001849","article-title":"ICTV Virus Taxonomy Profile: Poxviridae 2023","volume":"104","author":"McInnes","year":"2023","journal-title":"J. Gen. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Offerman, K., Carulei, O., van der Walt, A.P., Douglass, N., and Williamson, A.L. (2014). The complete genome sequences of poxviruses isolated from a penguin and a pigeon in South Africa and comparison to other sequenced avipoxviruses. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-463"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sarker, S., Athukorala, A., Bowden, T.R., and Boyle, D.B. (2021). Genomic Characterisation of a Novel Avipoxvirus Isolated from an Endangered Yellow-Eyed Penguin (Megadyptes antipodes). Viruses, 13.","DOI":"10.3390\/v13020194"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Carulei, O., Douglass, N., and Williamson, A.-L. (2017). Comparative analysis of avian poxvirus genomes, including a novel poxvirus from lesser flamingos (Phoenicopterus minor), highlights the lack of conservation of the central region. BMC Genom., 18.","DOI":"10.1186\/s12864-017-4315-0"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sarker, S., Bowden, T.R., and Boyle, D.B. (2022). Evidence of a Possible Viral Host Switch Event in an Avipoxvirus Isolated from an Endangered Northern Royal Albatross (Diomedea sanfordi). Viruses, 14.","DOI":"10.3390\/v14020302"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.meegid.2015.07.031","article-title":"Time scale evolution of avipoxviruses","volume":"35","author":"Bertagnoli","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1099\/jgv.0.001428","article-title":"Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses","volume":"101","author":"Majid","year":"2020","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ma, X., Zhang, M., Zhang, X., Qi, T., Zhang, W., Zhao, Y., Na, L., Zhang, Y., Wang, X.-F., and Wang, X. (2025). Construction and Immunogenicity Evaluation of a Recombinant Fowlpox Virus Expressing VP2 Gene of African Horse Sickness Virus Serotype 1. Microorganisms, 13.","DOI":"10.3390\/microorganisms13122807"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chen, S., Xu, N., Ta, L., Li, S., Su, X., Xue, J., Du, Y., Qin, T., and Peng, D. (2020). Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens. Vaccines, 8.","DOI":"10.3390\/vaccines8040623"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"588","DOI":"10.5187\/jast.2022.e30","article-title":"Evaluation of concurrent vaccinations with recombinant canarypox equine influenza virus and inactivated equine herpesvirus vaccines","volume":"64","author":"Lee","year":"2022","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s12088-017-0639-3","article-title":"Construction, Selection and Immunogenicity of Recombinant Fowlpox Candidate Vaccine Co-expressing HIV-1 gag and gp145","volume":"57","author":"Zhu","year":"2017","journal-title":"Indian J. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s12088-018-0728-y","article-title":"SIV-Specific Antibodies are Elicited by a Recombinant Fowlpox Virus Co-expressing SIV Gag and envT","volume":"58","author":"Zhu","year":"2018","journal-title":"Indian J. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/s41417-019-0112-z","article-title":"Phase I neoadjuvant study of intravesical recombinant fowlpox-GM-CSF (rF-GM-CSF) or fowlpox-TRICOM (rF-TRICOM) in patients with bladder carcinoma","volume":"27","author":"Portal","year":"2019","journal-title":"Cancer Gene Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s00262-022-03274-6","article-title":"Phase II trial of CV301 vaccine combined with atezolizumab in advanced urothelial carcinoma","volume":"72","author":"Sonpavde","year":"2022","journal-title":"Cancer Immunol. Immunother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1007\/s00705-019-04277-y","article-title":"Characterization of Iranian canarypox and pigeonpox virus strains","volume":"164","author":"Zarifi","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1007\/s11262-024-02084-5","article-title":"Isolation and identification of a pigeonpox virus strain and study on the integration of reticuloendotheliosis virus sequence","volume":"60","author":"Liu","year":"2024","journal-title":"Virus Genes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1080\/00071668.2019.1639141","article-title":"Insights into pathological and molecular characterization of avipoxviruses circulating in Egypt","volume":"60","author":"Lebdah","year":"2019","journal-title":"Br. Poult. Sci."},{"key":"ref_23","first-page":"417","article-title":"Molecular identification and Isolation of avian poxviruses from different bird species in Iran","volume":"80","author":"Eram","year":"2025","journal-title":"Arch. Razi Inst."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/03079457.2018.1554893","article-title":"Spotlight on avian pathology: Fowlpox virus","volume":"48","author":"Giotis","year":"2018","journal-title":"Avian Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104249","DOI":"10.1016\/j.psj.2024.104249","article-title":"Molecular and pathological screening of the current circulation of fowlpox and pigeon pox virus in backyard birds","volume":"103","author":"Mohamed","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e0088125","DOI":"10.1128\/mra.00881-25","article-title":"Genome sequence of fowlpox virus from a field outbreak in Bangladesh","volume":"14","author":"Mozumder","year":"2025","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1111\/tbed.14446","article-title":"Epidemic of cutaneous fowlpox in a na\u00efve population of chickens and turkeys in Austria: Detailed phylogenetic analysis indicates co-evolution of fowlpox virus with reticuloendotheliosis virus","volume":"69","author":"Matos","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Akther, M., Akter, S.H., Sarker, S., Aleri, J.W., Annandale, H., Abraham, S., and Uddin, J.M. (2023). Global Burden of Lumpy Skin Disease, Outbreaks, and Future Challenges. Viruses, 15.","DOI":"10.3390\/v15091861"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Parvin, R., Chowdhury, E.H., Islam, M.T., Begum, J.A., Nooruzzaman, M., Globig, A., Dietze, K., Hoffmann, B., and Tuppurainen, E. (2022). Clinical Epidemiology, Pathology, and Molecular Investigation of Lumpy Skin Disease Outbreaks in Bangladesh during 2020\u20132021 Indicate the Re-Emergence of an Old African Strain. Viruses, 14.","DOI":"10.3390\/v14112529"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.1111\/jvim.17135","article-title":"Persistence of maternal antibodies against goat pox virus in goat kids","volume":"38","author":"Abdollahi","year":"2024","journal-title":"J. Vet. Intern. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.vetmic.2011.01.011","article-title":"The phylogenetic analysis of avipoxvirus in New Zealand","volume":"150","author":"Ha","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4938","DOI":"10.1128\/JVI.03183-12","article-title":"Worldwide Phylogenetic Relationship of Avian Poxviruses","volume":"87","author":"Gyuranecz","year":"2013","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Atta, S., Asif, K., O\u2019Rourke, D., Legione, A.R., Shil, P., Marenda, M.S., and Noormohammadi, A.H. (2021). Whole-genome based strain identification of fowlpox virus directly from cutaneous tissue and propagated virus. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0261122"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"199218","DOI":"10.1016\/j.virusres.2023.199218","article-title":"Genomic characteristics of an avipoxvirus 282E4 strain","volume":"336","author":"Deng","year":"2023","journal-title":"Virus Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Sarker, S., Das, S., Lavers, J.L., Hutton, I., Helbig, K., Imbery, J., Upton, C., and Raidal, S.R. (2017). Genomic characterization of two novel pathogenic avipoxviruses isolated from pacific shearwaters (Ardenna spp.). BMC Genom., 18.","DOI":"10.1186\/s12864-017-3680-z"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"D708","DOI":"10.1093\/nar\/gkx932","article-title":"Virus taxonomy: The database of the International Committee on Taxonomy of Viruses (ICTV)","volume":"46","author":"Lefkowitz","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virusres.2018.10.017","article-title":"Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus","volume":"260","author":"Joshi","year":"2019","journal-title":"Virus Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1099\/vir.0.19568-0","article-title":"Comparison of the genome sequence of FP9, an attenuated, tissue culture-adapted European strain of Fowlpox virus, with those of virulent American and European viruses","volume":"85","author":"Laidlaw","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1080\/03079459994434","article-title":"Avian host spectrum of avipoxviruses","volume":"28","author":"Bolte","year":"2010","journal-title":"Avian Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1111\/tbed.13668","article-title":"An atypical clinicopathological manifestation of fowlpox virus associated with reticuloendotheliosis virus in commercial laying hen flocks in Brazil","volume":"67","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3856","DOI":"10.1038\/s41467-024-48180-w","article-title":"Predicting vaccine effectiveness for mpox","volume":"15","author":"Berry","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.medcli.2023.01.001","article-title":"Vaccines against monkeypox","volume":"160","author":"Reina","year":"2023","journal-title":"Med. Cl\u00ednica (Engl. Ed.)"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1007\/s11262-020-01799-5","article-title":"Construction and immune protection evaluation of recombinant virus expressing Newcastle disease virus F protein by the largest intergenic region of fowlpox virus NX10","volume":"56","author":"Zhao","year":"2020","journal-title":"Virus Genes"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/393\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:24:12Z","timestamp":1776428652000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/4\/393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,17]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["vetsci13040393"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13040393","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,17]]}}}