{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:13:42Z","timestamp":1785413622454,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","award":["243745T0"],"award-info":[{"award-number":["243745T0"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","award":["FF(S-KU)5.66"],"award-info":[{"award-number":["FF(S-KU)5.66"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]},{"award":["243745T0"],"award-info":[{"award-number":["243745T0"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]},{"award":["FF(S-KU)5.66"],"award-info":[{"award-number":["FF(S-KU)5.66"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Streptococcus suis is a major zoonotic pathogen, with increasing human cases in Nakhon Ratchasima Province, northeastern Thailand. This study aimed to assess residents\u2019 knowledge, attitudes, and practices (KAP) regarding infection risk and to characterize S. suis isolates from pigs. A structured questionnaire was administered to 500 residents to evaluate awareness and behaviors related to S. suis infection. Nasopharyngeal swabs were collected from slaughtered pigs at three slaughterhouses in Nakhon Ratchasima Province. Presumptive Streptococcus isolates were confirmed as S. suis by PCR and further characterized by molecular serotyping, virulence-associated gene profiling, and multilocus sequence typing (MLST). Among residents, mean scores suggested generally high levels of KAP; however, misunderstandings regarding transmission persisted. Residents expressed strong readiness to reduce infection risk. Of 285 pig samples, 122 (42.8%) were positive for S. suis, representing 16 serotypes. Serotype 9 (10.3%) predominated with several serotypes that have been reported in human infections. The predominant virulence-associated gene profile was mrp\u2212\/sly\u2212\/epf\u2212 (77.4%). MLST identified eight STs with five novel STs (ST3147-3151), indicating substantial genetic diversity. Persistent misconceptions despite generally acceptable KAP levels highlight the need for targeted public health education. The high prevalence and genetic variability of S. suis in pigs support ongoing surveillance to monitor potentially pathogenic strains.<\/jats:p>","DOI":"10.3390\/vetsci13050458","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T19:10:52Z","timestamp":1778267452000},"page":"458","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Public Awareness and Molecular Characterization of Streptococcus suis in a High-Incidence Region of Thailand"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5869-5714","authenticated-orcid":false,"given":"Perm","family":"Premphoolsawat","sequence":"first","affiliation":[{"name":"Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khomson","family":"Satchasataporn","sequence":"additional","affiliation":[{"name":"Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-2358","authenticated-orcid":false,"given":"Thitichai","family":"Jarudecha","sequence":"additional","affiliation":[{"name":"Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamonwan","family":"Lunha","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suganya","family":"Yongkiettrakul","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-3656","authenticated-orcid":false,"given":"Anusak","family":"Kerdsin","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6875-0498","authenticated-orcid":false,"given":"Daisuke","family":"Takamatsu","sequence":"additional","affiliation":[{"name":"<sans-serif>Department of Bacterial Diseases,<\/sans-serif> National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba 305-0856, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0098-2361","authenticated-orcid":false,"given":"Nattakan","family":"Meekhanon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.4161\/viru.28595","article-title":"Streptococcus suis infection: An emerging\/reemerging challenge of bacterial infectious diseases?","volume":"5","author":"Feng","year":"2014","journal-title":"Virulence"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Panpaeng, C., Kamolvit, W., Karaketklang, K., and Jitmuang, A. 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