{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:31:44Z","timestamp":1785407504784,"version":"3.56.0"},"reference-count":28,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402967"],"award-info":[{"award-number":["32402967"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32402967"],"award-info":[{"award-number":["32402967"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025MD774143"],"award-info":[{"award-number":["2025MD774143"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025MD774143"],"award-info":[{"award-number":["2025MD774143"]}],"id":[{"id":"https:\/\/ror.org\/0426zh255","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015308","name":"Science and Technology Department of Xinjiang Uyghur Autonomous Region","doi-asserted-by":"publisher","award":["2025D01A77"],"award-info":[{"award-number":["2025D01A77"]}],"id":[{"id":"10.13039\/501100015308","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025D01A77"],"award-info":[{"award-number":["2025D01A77"]}],"id":[{"id":"https:\/\/ror.org\/02vtvez67","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>This study aimed to evaluate the therapeutic effects of platelet-rich plasma (PRP) and Cervus and Cucumis polypeptide (CCP) injections in rats with post-traumatic osteoarthritis (OA). The model was established by transection of the anterior cruciate ligament, and the animals were subsequently treated with PRP and CCP. Articular cartilage degeneration was assessed through gross morphological observation, histopathological staining, and a standardized scoring system. Concurrently, pain-related behaviors, joint swelling, levels of inflammatory cytokines, and markers associated with extracellular matrix degradation were measured. The results demonstrated that, compared with the OA model group, PRP and CCP exhibited varying degrees of functional improvement, specifically, a reduction in pain-related behaviors and an alleviation of joint swelling. Furthermore, cartilage morphological damage was diminished, inflammatory marker levels decreased, and indicators of extracellular matrix degradation were attenuated. Histopathological examination of liver and kidney tissues revealed no apparent abnormalities. This study provides valuable experimental evidence for further treatment strategies for OA.<\/jats:p>","DOI":"10.3390\/vetsci13060506","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T16:06:08Z","timestamp":1779465968000},"page":"506","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combined PRP and CCP Therapy Suppresses Inflammation and Protects Cartilage in Post-Traumatic Osteoarthritis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3809-1131","authenticated-orcid":false,"given":"Tianwen","family":"Ma","sequence":"first","affiliation":[{"name":"National Postdoctoral Research Workstation, Zhaosu Xiyu Horse Industry Co., Ltd., Yili 835699, China"},{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"},{"name":"Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongti","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Li","sequence":"additional","affiliation":[{"name":"National Postdoctoral Research Workstation, Zhaosu Xiyu Horse Industry Co., Ltd., Yili 835699, China"},{"name":"Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongsheng","family":"Qiu","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhui","family":"Ma","sequence":"additional","affiliation":[{"name":"National Postdoctoral Research Workstation, Zhaosu Xiyu Horse Industry Co., Ltd., Yili 835699, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Li","sequence":"additional","affiliation":[{"name":"National Postdoctoral Research Workstation, Zhaosu Xiyu Horse Industry Co., Ltd., Yili 835699, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8560-4564","authenticated-orcid":false,"given":"Baoming","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Graves, J.L., McKenzie, B.A., Koch, Z., Naka, A., Spofford, N., and Morrison, J. (2023). Body weight, gonadectomy, and other risk factors for diagnosis of osteoarthritis in companion dogs. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1275964"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1302\/2046-3758.111.2000132","article-title":"The horse as a model of naturally occurring osteoarthritis","volume":"1","author":"McIlwraith","year":"2012","journal-title":"Bone Jt. Res."},{"key":"ref_3","first-page":"947","article-title":"The Therapeutic Role of Hyaluronic Acid in Veterinary Medicine: A Narrative Review of Clinical Applications","volume":"45","author":"Cihan","year":"2025","journal-title":"Pak. Vet. J."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Qiu, Z., Wang, J., Zhang, Y., Liu, X., Wei, C., and Ma, T. (2025). Extracorporeal shock wave therapy for equine musculoskeletal disorders: From biological mechanisms to clinical applications. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1719123"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1186\/s13018-023-04119-3","article-title":"Effects and action mechanisms of individual cytokines contained in PRP on osteoarthritis","volume":"18","author":"Wang","year":"2023","journal-title":"J. Orthop. Surg. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1002\/pmrj.13139","article-title":"Postinjection protocols following platelet-rich plasma administration for knee osteoarthritis: A systematic review","volume":"16","author":"Levine","year":"2024","journal-title":"PMR"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1177\/03635465231224463","article-title":"Platelet-Rich Plasma Versus Alternative Injections for Osteoarthritis of the Knee: A Systematic Review and Statistical Fragility Index-Based Meta-analysis of Randomized Controlled Trials","volume":"52","author":"Oeding","year":"2024","journal-title":"Am. J. Sports Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1177\/03635465241246524","article-title":"PRP Injections for the Treatment of Knee Osteoarthritis: The Improvement Is Clinically Significant and Influenced by Platelet Concentration: A Meta-analysis of Randomized Controlled Trials","volume":"53","author":"Bensa","year":"2025","journal-title":"Am. J. Sports Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"179","DOI":"10.2147\/OAJSM.S567695","article-title":"Current Perspectives on Platelet-Rich Plasma Injections for Knee Osteoarthritis: How to Optimize Clinical Outcomes","volume":"16","author":"Dhillon","year":"2025","journal-title":"Open Access J. Sports Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Tram\u015b, E., Malesa, K., Pomianowski, S., and Kami\u0144ski, R. (2022). Role of Platelets in Osteoarthritis-Updated Systematic Review and Meta-Analysis on the Role of Platelet-Rich Plasma in Osteoarthritis. Cells, 11.","DOI":"10.3390\/cells11071080"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"154","DOI":"10.5603\/fhc.100179","article-title":"Platelet-rich plasma ameliorates cartilage degradation in rat models of osteoarthritis via the OPG\/RANKL\/RANK system","volume":"62","author":"Wu","year":"2024","journal-title":"Folia Histochem. Cytobiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"460827","DOI":"10.1016\/j.chroma.2019.460827","article-title":"Simultaneously quantitative analysis of peptides and chemical components in Cervus and Cucumis polypeptide injection (Songmeile\u00ae) using reversed phase liquid chromatography-hydrophilic interaction liquid chromatography-tandem mass spectrometry","volume":"1617","author":"Liu","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"958908","DOI":"10.1155\/2014\/958908","article-title":"Effect of cervus and cucumis peptides on osteoblast activity and fracture healing in osteoporotic bone","volume":"2014","author":"Wang","year":"2014","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e000331","DOI":"10.1136\/lupus-2019-000331","article-title":"Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis","volume":"6","author":"Lin","year":"2019","journal-title":"Lupus Sci. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0254-6272(16)30006-1","article-title":"Efficacy and safety evaluation of intravenous infusion of cervus and cucumis polypeptides for treatment of avascular necrosis of the femoral head: A randomized clinical trial","volume":"36","author":"Wei","year":"2016","journal-title":"J. Tradit. Chin. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.jare.2025.01.010","article-title":"Antioxidant taurine inhibits chondrocyte ferroptosis through upregulation of OGT\/Gpx4 signaling in osteoarthritis induced by anterior cruciate ligament transection","volume":"77","author":"Zhou","year":"2025","journal-title":"J. Adv. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e1169","DOI":"10.1002\/iid3.1169","article-title":"The Nrf2\/HO-1 pathway participates in the antiapoptotic and anti-inflammatory effects of platelet-rich plasma in the treatment of osteoarthritis","volume":"12","author":"Du","year":"2024","journal-title":"Immun. Inflamm. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e70113","DOI":"10.1111\/jpi.70113","article-title":"Melatonin Improves Diabetic-Accelerated Experimental Osteoarthritis by Inhibiting NLRP3-Mediated Chondrocyte Pyroptosis via PKG\/Nrf2","volume":"78","author":"Yang","year":"2026","journal-title":"J. Pineal Res."},{"key":"ref_19","first-page":"100090","article-title":"The role of inflammatory mediators and matrix metalloproteinases (MMPs) in the progression of osteoarthritis","volume":"13","author":"Mukherjee","year":"2024","journal-title":"Biomater. Biosyst."},{"key":"ref_20","first-page":"138","article-title":"BMP-9 and TGF-\u03b23 synergistically regulate chondrogenic pathways in bovine synovial fluid-derived Mesenchymal Stem Cells (BSF-MSCs) in transwell co-culture with chondrocytes","volume":"45","author":"Erdogan","year":"2025","journal-title":"Pak. Vet. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1038\/s41598-025-88918-0","article-title":"Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration","volume":"15","author":"Fiz","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wu, W.S., Chen, L.R., and Chen, K.H. (2025). Platelet-Rich Plasma (PRP): Molecular Mechanisms, Actions and Clinical Applications in Human Body. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262110804"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1177\/03635465231212668","article-title":"The Combined Intraosseous Administration of Orthobiologics Outperformed Isolated Intra-articular Injections in Alleviating Pain and Cartilage Degeneration in a Rat Model of MIA-Induced Knee Osteoarthritis","volume":"52","author":"Zhang","year":"2024","journal-title":"Am. J. Sports Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e21222","DOI":"10.1097\/MD.0000000000021222","article-title":"Cervus and cucumis peptides combined umbilical cord mesenchymal stem cells therapy for rheumatoid arthritis","volume":"99","author":"Qi","year":"2020","journal-title":"Medicine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1186\/s12951-025-03591-9","article-title":"Exosomes derived from platelet-rich plasma alleviate synovial inflammation by enhancing synovial lymphatic function","volume":"23","author":"Liao","year":"2025","journal-title":"J. Nanobiotechnology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1080\/03008207.2021.1962314","article-title":"Effect of bone marrow mesenchymal stem cell transplantation combined with lugua polypeptide injection on osteoarthritis in rabbit knee joint","volume":"63","author":"Huang","year":"2022","journal-title":"Connect. Tissue Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"120149","DOI":"10.1016\/j.jep.2025.120149","article-title":"Antler base (Cervus nippon Temminck) peptides modulate the NLRP3 inflammatory pyroptosis and Nrf2\/HO-1\/NQO1 signaling pathways to ameliorate osteoarthritis: A structural and mechanistic study","volume":"351","author":"Sun","year":"2025","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.reth.2024.11.013","article-title":"Platelet-rich plasma combined with isometric quadriceps contraction regulates autophagy in chondrocytes via the PI3K\/AKT\/mTOR pathway to promote cartilage repair in knee osteoarthritis","volume":"28","author":"Cheng","year":"2025","journal-title":"Regen. Ther."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/506\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T04:13:54Z","timestamp":1780200834000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,22]]},"references-count":28,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vetsci13060506"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13060506","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,22]]}}}