{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:14:33Z","timestamp":1787030073579,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nextmune AB, Stockholm, Sweden"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Canine atopic dermatitis (AD) is a common allergic skin disease for which identifying allergen sensitization via IgE serological or intradermal testing is necessary to implement allergen-specific immunotherapy. Because serum IgE has a short half-life and circulating levels fluctuate with environmental allergen exposure, the timing of blood sampling may influence serological test outcomes. This cross-sectional study assessed seasonal variation in allergen-specific IgE concentrations and seropositivity rates across pollen and mite allergen categories in Scandinavian dogs suspected of allergic disease. PAX multiplex macroarray results from 5014 canine sera submitted by veterinarians across Denmark, Norway, and Sweden over one full year were retrospectively analyzed for 17 allergens, including tree, grass, and weed pollens, house dust mites, and storage mites. Mean sIgE levels showed statistically significant but relatively small seasonal variation. Seropositivity rates, however, showed clearer patterns: seropositivity for tree and weed pollen was highest in spring and summer, and that for house dust mite peaked in autumn and winter, while storage mite sensitization rates showed the opposite trend. These results confirm that IgE serological test results in dogs are affected by the sampling season and emphasize the importance of considering timing when collecting and interpreting IgE serological tests in atopic dogs.<\/jats:p>","DOI":"10.3390\/vetsci13060522","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T08:00:36Z","timestamp":1779955236000},"page":"522","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Seasonality of Serum Allergen-Specific IgE Levels in Scandinavian Dogs Suspected of Allergy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6697-7170","authenticated-orcid":false,"given":"Tilda","family":"B\u00f6rjesson","sequence":"first","affiliation":[{"name":"AniCura Falu Djursjukhus, Samuelsdals\u00e4gen 2B, 791 61 Falun, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Streets","sequence":"additional","affiliation":[{"name":"Nextmune UK Labs, Unit 651, Street 5, Thorp Arch Trading Estate, Wetherby LS23 7FZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1399-0034","authenticated-orcid":false,"given":"Thierry","family":"Olivry","sequence":"additional","affiliation":[{"name":"Nextmune AB, Riddargatan 17, 114 57 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/vde.13183","article-title":"Introduction to the ICADA 2023 Canine Atopic Dermatitis Pathogenesis Review Articles and Updated Definition","volume":"35","author":"Eisenschenk","year":"2024","journal-title":"Vet. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hensel, P., Santoro, D., Favrot, C., Hill, P., and Griffin, C. (2015). Canine Atopic Dermatitis: Detailed Guidelines for Diagnosis and Allergen Identification. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0515-5"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1365-3164.2010.00925.x","article-title":"Breed-Associated Phenotypes in Canine Atopic Dermatitis","volume":"22","author":"Wilhelm","year":"2011","journal-title":"Vet. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"79-e24","DOI":"10.1111\/vde.12196","article-title":"Review: Clinical and Histological Manifestations of Canine Atopic Dermatitis","volume":"26","author":"Bizikova","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/s41577-022-00786-1","article-title":"Allergen Immunotherapy: Past, Present and Future","volume":"23","author":"Durham","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.2460\/javma.22.12.0576","article-title":"A Systematic Review of Allergen Immunotherapy, a Successful Therapy for Canine Atopic Dermatitis and Feline Atopic Skin Syndrome","volume":"261","author":"Mueller","year":"2023","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Morales-Romero, R., Gonzalez-Dominguez, M.S., S\u00e1nchez, J., Correa-Valencia, N.M., and Maldonado-Estrada, J.G. (2025). Efficacy of Diagnostic Testing for Allergen Sensitization in Canine Atopic Dermatitis: A Systematic Review. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1551207"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1016\/j.jaip.2018.08.032","article-title":"Allergen Extracts for in Vivo Diagnosis and Treatment of Allergy: Is There a Future?","volume":"6","author":"Valenta","year":"2018","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Welters, M., Mas-Fontao, A., Auxilia, S.T., and Olivry, T. (2025). Composition Heterogeneity and Low-Molecular-Weight Allergen Content of Dermatophagoides farinae House Dust Mite Allergen Extracts Used in Veterinary Medicine. Vet. Sci., 12.","DOI":"10.3390\/vetsci12090824"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1515\/cclm-2024-0305","article-title":"Molecular Allergology: A Clinical Laboratory Tool for Precision Diagnosis, Stratification and Follow-up of Allergic Patients","volume":"62","author":"Giusti","year":"2024","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.jaci.2025.01.014","article-title":"Molecular Allergy Diagnosis Enabling Personalized Medicine","volume":"156","author":"Matricardi","year":"2025","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40413-018-0186-3","article-title":"Extended IgE Profile Based on an Allergen Macroarray: A Novel Tool for Precision Medicine in Allergy Diagnosis","volume":"11","author":"Heffler","year":"2018","journal-title":"World Allergy Organ. J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lupinek, C., Forstenlechner, P., Ringauf, A., Campana, R., Salihu, A., Aumayr, M., and Mittermann, I. (2025). Big Data-Driven Evolution of a Diagnostic Multiplex IgE-Test: Enhancing Accuracy and Efficacy in Allergy Diagnostics. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26094249"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.jaci.2016.04.056","article-title":"Half-Life of IgE in Serum and Skin: Consequences for Anti-IgE Therapy in Patients with Allergic Disease","volume":"139","author":"Lawrence","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1111\/cea.13895","article-title":"Seasonal Variation in Total and Pollen-Specific Immunoglobulin E Levels in the European Community Respiratory Health Survey","volume":"51","author":"Lam","year":"2021","journal-title":"Clin. Exp. Allergy"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/0091-6749(93)90343-E","article-title":"The Effects of Season, Climate, and Air-Conditioning on the Prevalence of Dermatophagoides Mite Allergens in Household Dust","volume":"91","author":"Lintner","year":"1993","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1159\/000342444","article-title":"Specific IgE Levels to Dermatophagoides pteronyssinus Are Associated with Meteorological Factors","volume":"160","author":"Pons","year":"2013","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/0091-6749(78)90213-0","article-title":"The Radioallergosorbent Test in the Diagnosis of Canine Atopic Disease","volume":"62","author":"Halliwell","year":"1978","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1046\/j.0959-4493.2001.00277.x","article-title":"Seasonal Atopic Dermatitis in Dogs Sensitive to a Major Allergen of Japanese Cedar (Cryptomeria japonica) Pollen","volume":"13","author":"Masuda","year":"2002","journal-title":"Vet. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13028-014-0081-z","article-title":"Prevalence of and Risk Factors for Increased Serum Levels of Allergen-Specific IgE in a Population of Norwegian Dogs","volume":"56","author":"Bjelland","year":"2014","journal-title":"Acta Vet. Scand."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chermprapai, S., and Thengchaisri, N. (2020). A Descriptive Study of Allergen-Specific IgE Serological Tests for Canine Atopic Dermatitis in Thailand. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02684-x"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/vde.13309","article-title":"Prevalence of Immunoglobulin E Sensitisation to Mites and Pollens in 25,451 French Dogs from 2016 to 2022","volume":"36","author":"Drouet","year":"2025","journal-title":"Vet. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1365-3164.1992.tb00179.x","article-title":"The Influence of Oral Corticosteroids or Declining Allergen Exposure on Serologic Allergy Test Results","volume":"3","author":"Miller","year":"1992","journal-title":"Vet. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"347-e96","DOI":"10.1111\/vde.12974","article-title":"Multivariable Analysis of the Influence of Cross-Reactive Carbohydrate Determinant Inhibition and Other Factors on Intradermal and Serological Allergen Test Results: A Prospective, Multicentre Study","volume":"32","author":"Canning","year":"2021","journal-title":"Vet. Dermatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1136\/vr.100710","article-title":"Characterisation of Dog Sensitisation to Grass Pollen in Western France from 1999 to 2010","volume":"172","author":"Roussel","year":"2013","journal-title":"Vet. Rec."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Olivry, T., Fontao, A.M., Aumayr, M., Ivanovova, N.P., Mitterer, G., and Harwanegg, C. (2024). Validation of a Multiplex Molecular Macroarray for the Determination of Allergen-Specific IgE Sensitizations in Dogs. Vet. Sci., 11.","DOI":"10.20944\/preprints202408.0601.v1"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Olivry, T., Mas Fontao, A., Widorn, L., and Mueller, R.S. (2024). Evaluating the Pathogenic Potential of IgE Targeting Cross-Reactive Carbohydrate Determinants in Dogs. Animals, 14.","DOI":"10.3390\/ani14223275"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s10453-023-09806-6","article-title":"Allergenic Pollen Seasons and Regional Pollen Calendars for Norway","volume":"40","author":"Frisk","year":"2024","journal-title":"Aerobiologia"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lind, T., Ekebom, A., Alm K\u00fcbler, K., \u00d6stensson, P., Bellander, T., and L\u00f5hmus, M. (2016). Pollen Season Trends (1973\u20132013) in Stockholm Area, Sweden. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0166887"},{"key":"ref_30","first-page":"486","article-title":"A Multi-Centre Analysis of Serum IgE Levels in Atopic Dermatitis","volume":"69","author":"Yu","year":"2024","journal-title":"Indian J. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1136\/vr.149.14.424","article-title":"Examination of Faecal Samples as a Method of Identifying Pollen Exposure in Dogs","volume":"149","author":"Fraser","year":"2001","journal-title":"Vet. Rec."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1365-3164.2008.00654.x","article-title":"Assessment of Cross-Reactivity among Five Species of House Dust and Storage Mites","volume":"19","author":"Saridomichelakis","year":"2008","journal-title":"Vet. Dermatol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chruszcz, M., Kapingidza, A.B., Dolamore, C., and Kowal, K. (2018). A Robust Method for the Estimation and Visualization of IgE Cross-Reactivity Likelihood between Allergens Belonging to the Same Protein Family. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0208276"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"100667","DOI":"10.1016\/j.jacig.2026.100667","article-title":"Dermatophagoides pteronyssinus in Ambient Air Bioaerosols","volume":"5","author":"Gusareva","year":"2026","journal-title":"J. Allergy Clin. Immunol. Glob."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s40629-022-00231-8","article-title":"Biology of House Dust Mites and Storage Mites","volume":"31","author":"Bergmann","year":"2022","journal-title":"Allergo J. Int."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/522\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T08:42:35Z","timestamp":1779957755000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,28]]},"references-count":35,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vetsci13060522"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13060522","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,28]]}}}